<abstract xmlns="http://www.w3.org/1999/xhtml">Pemetrexed (PMX) is an antifolate drug utilized in the treatment of non-small cell lung cancer. For studies of potential macromolecular carriers for PMX, fast and precise methods were developed to determine the bound and free drug contained in investigated conjugate preparations. The analysis of the total amount of PMX in conjugates was based on absorption spectrophotometry. The linearity was found in the range of 4.697–46.97 μmol L−1 PMX. The limit of quantitation was 1.070 μmol L−1. The method for the analysis of unbound PMX was based on size-exclusion chromatography and detection at 225 nm. This method shows linear range of 2.230–223.0 μmol L−1. <italic>LOQ</italic> was 0.539 μmol L−1. The proposed methods can be used both for the characterization of the polysaccharide based conjugates of PMX and for the determination of conjugate drug release profiles.</abstract>

Pemetrexed (PMX) is an antifolate drug utilized in the treatment of non-small cell lung cancer. For studies of potential macromolecular carriers for PMX, fast and precise methods were developed to determine the bound and free drug contained in investigated conjugate preparations. The analysis of the total amount of PMX in conjugates was based on absorption spectrophotometry. The linearity was found in the range of 4.697–46.97 μmol L−1 PMX. The limit of quantitation was 1.070 μmol L−1. The method for the analysis of unbound PMX was based on size-exclusion chromatography and detection at 225 nm. This method shows linear range of 2.230–223.0 μmol L−1. LOQ was 0.539 μmol L−1. The proposed methods can be used both for the characterization of the polysaccharide based conjugates of PMX and for the determination of conjugate drug release profiles.

Methods for preliminary determination of pemetrexed in macromolecular drug-carrier systems

”NeoLek” Laboratory, Department of Experimental Oncology, Ludwik, Hirszfeld Institute of Immunology and Experimental Therapy, PAS, Wrocław 53-114, Poland

Jan Dlugosz Academy, Al. Armii Krajowej 13/15, Częstochowa 42-201, Poland

Acta Pharmaceutica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Pemetrexed (PMX) is an antifolate drug utilized in the treatment of non-small cell lung cancer. For studies of potential macromolecular carriers for PMX, fast...

Methods for preliminary determination of pemetrexed in macromolecular drug-carrier systems

Published Online: Mar 07, 2016

Page range: 147 - 153

Accepted: Sep 17, 2015

DOI: https://doi.org/10.1515/acph-2016-0001

Keywords
pemetrexed, macromolecular drug-carrier, size-exclusion chromatography, ultraviolet spectroscopy

© 2016 Jarosław Ciekot et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Methods for preliminary determination of pemetrexed in macromolecular drug-carrier systems

Published Online: Mar 07, 2016

Page range: 147 - 153

Accepted: Sep 17, 2015

DOI: https://doi.org/10.1515/acph-2016-0001

Keywordspemetrexed, macromolecular drug-carrier, size-exclusion chromatography, ultraviolet spectroscopy

© 2016 Jarosław Ciekot et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
pemetrexed, macromolecular drug-carrier, size-exclusion chromatography, ultraviolet spectroscopy