<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Detection of mutations in cancer is particularly important in terms of proper treatment and targeted therapy. The aim of this study was the comparison of two methods: allele-specific PCR (AS-PCR) and dideoxysequencing applied for the identification of <italic>BRAF</italic> gene mutations in wild-type gastrointestinal stromal tumors (WT GISTs). We have optimized the conditions for the detection V600E mutation representing the c.1799 T>A substitution by AS-PCR and have used dideoxysequencing to verify our results. In nine cases, we were able to detect the mutation by AS-PCR approach; however, the mutations have been confirmed by dideoxysequencing in four cases only. AS-PCR is fast and low cost method for the detection of V600E mutation which was validated as a sensitive assay for the identification of the most common <italic>BRAF</italic> mutation in DNA extracted from paraffin-embedded tissue of WT GISTs.</p></abstract>

Detection of mutations in cancer is particularly important in terms of proper treatment and targeted therapy. The aim of this study was the comparison of two methods: allele-specific PCR (AS-PCR) and dideoxysequencing applied for the identification of BRAF gene mutations in wild-type gastrointestinal stromal tumors (WT GISTs). We have optimized the conditions for the detection V600E mutation representing the c.1799 T>A substitution by AS-PCR and have used dideoxysequencing to verify our results. In nine cases, we were able to detect the mutation by AS-PCR approach; however, the mutations have been confirmed by dideoxysequencing in four cases only. AS-PCR is fast and low cost method for the detection of V600E mutation which was validated as a sensitive assay for the identification of the most common BRAF mutation in DNA extracted from paraffin-embedded tissue of WT GISTs.

Limits of Dideoxysequencing in the Detection of Somatic Mutations in Gastrointestinal Stromal Tumors

Department of Molecular Biology, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava

Department of Pathological Anatomy, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava

Biomedical Center Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava

Acta Medica Martiniana

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Detection of mutations in cancer is particularly important in terms of proper treatment and targeted therapy. The aim of this study was the comparison of two...

Limits of Dideoxysequencing in the Detection of Somatic Mutations in Gastrointestinal Stromal Tumors

Published Online: Jan 22, 2016

Page range: 13 - 20

Received: Jul 02, 2015

Accepted: Aug 31, 2015

DOI: https://doi.org/10.1515/acm-2015-0013

Keywords
wild-type GIST, allele-specific PCR, dideoxysequencing

© 2015 K Jasek et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Limits of Dideoxysequencing in the Detection of Somatic Mutations in Gastrointestinal Stromal Tumors

Published Online: Jan 22, 2016

Page range: 13 - 20

Received: Jul 02, 2015

Accepted: Aug 31, 2015

DOI: https://doi.org/10.1515/acm-2015-0013

Keywordswild-type GIST, allele-specific PCR, dideoxysequencing

© 2015 K Jasek et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
wild-type GIST, allele-specific PCR, dideoxysequencing