<abstract xmlns="http://www.w3.org/1999/xhtml">

Encapsulated lipid nanoemulsions (EO-LNs) from garlic oil, lemongrass oil and mustard oil were prepared by a combined method of homogenization and sonication with the aim to generate highly effective formulations against <italic>Fusarium incarnatum</italic> (laboratory bioassays). Their combined binary and ternary formulations (preparations by mixing an equal volume ratio of each EO-LNs) were also tested. The synergistic/additive/antagonistic antifungal effect of the EOs (under nanoform) in their combined formulations was determined using the SynergyFinder software with the Bliss independence model. Results revealed the synergistic effect of the combined binary and ternary formulations of garlic oil-encapsulated LNs (NaG), lemongrass oil-encapsulated LNs (NaL) and mustard oil-encapsulated LNs (NaM). Furthermore, the ternary combination, at the same concentration of each constituent EO, had higher antifungal activity than the binary combinations. Nonetheless, at 600 times dilution the NaLG (binary combination) inhibited 96% the mycelial growth of <italic>F. incarnatum</italic>, which was significantly higher than the efficacy of NaMLG (ternary combination) in the same dilution. This could be possibly attributed to the 1.5-time higher concentration of each constituent EO in the binary combination compared to that in the ternary formulation. In addition, NaLG, even at the high EO concentration of 0.4 g/L, did not show any phytotoxicity symptoms on lettuce plants.
</abstract>

Evaluation of <italic>in-vitro</italic> antifungal activity against <italic>Fusarium incarnatum</italic> of binary and ternary combinations of lemongrass, garlic and mustard oil-encapsulated lipid nanoemulsions

<abstract xmlns="http://www.w3.org/1999/xhtml">

Salinity is an agricultural and eco-environmental problem worldwide that decreases crop production. Endophytic fungi have been shown to improve plant tolerance to stressful conditions. The purpose of the paper is to examine the efficiency of <italic>Embellisia phragmospora</italic>, <italic>Fusarium equiseti</italic> and <italic>Fusarium graminearum</italic> to improve tolerance of pot-grown barley in greenhouse under different levels of soil salinity (2.5, 8 and 14dS/m) by estimating growth, relative water content, mineral nutrition, photosynthetic pigments biosynthesis, proline and sugar levels. Results showed that <italic>E</italic>. <italic>phragmospora</italic> increased barley emergence rate to 66.7% compared to 60% recorded by non-colonized barley under 14dS/m soil salinity. The tested endophytes increased barley root length, shoot and root dry weights under salt stress. Endophytic fungi reduced Na+ accumulation and improved K+ uptake in barely under salinity. <italic>Fusarium equiseti</italic> and <italic>F. graminearum</italic>-inoculated barley increased proline content under salinity. <italic>Fusarium graminearum</italic>-colonized barley showed the highest sugar content under salt stress. Our findings demonstrate the feasibility of endophytic fungi bio-inoculation in improvement of barley tolerance to salt stress, which qualify them to be a potent tool to provide substantial benefits to crops for sustainable agriculture.
</abstract>

Plant Protection Laboratory, Abdelhamid IBN Badis - Mostaganem University

Unraveling the role of endophytic fungi in barley salt-stress tolerance

<abstract xmlns="http://www.w3.org/1999/xhtml">

The scale insect <italic>Stotzia ephedrae</italic> (Newstead) (Hemiptera: Coccomorpha: Coccidae) was recorded for the first time in Greece οn 20 April 2021. It was found on <italic>Ephedra foeminea</italic> Forssk. (Ephedraceae: Gnetales) in Athens. From preliminary studies it was found that <italic>S. ephedrae</italic> is an oviparous, bi-parental species completing one generation per year.
</abstract>

Laboratory of Agricultural Entomology and Zoology

Department of Agriculture, School of Agriculture and Food

Laboratory of Floriculture and Landscape Architecture, Department of Agriculture, School of Agriculture and Food

First record of the scale insect <italic>Stotzia ephedrae</italic> in Greece

<abstract xmlns="http://www.w3.org/1999/xhtml">

The scale insect <italic>Lineaspis striata</italic> (Newstead) (Hemiptera: Coccomorpha: Diaspididae) was recorded for the first time infesting a species of the family Pinaceae. Heavily infested ornamental shrubs of <italic>Picea glauca</italic> “Conica” by pre-ovipostiting and oviposting female adults of <italic>L. striata</italic>, were found in March 2018 and by a minor population consisting of pre-ovipositing female adults in February 2021, in Attica, Greece. The scale was settled on the base of the needles of the host plant.
</abstract>

First record of the scale insect <italic>Lineaspis striata</italic> on <italic>Picea glauca</italic> “Conica”

<abstract xmlns="http://www.w3.org/1999/xhtml">

With a continuously growing human population on our planet, the chemical burden of our environment is also growing. In addition, the resistance of microorganisms, like bacteria, to widely used chemicals is evident. Therefore, the application of bactericidal products that reduce the risks for development of resistance as well as the environment and human safety is of great benefit. In this work, we have screened the essential oil extracted from plants of <italic>Laurus nobilis</italic> L. (laurel) grown at the base of the Greek mountain Olympus for its antimicrobial activity against two strains of the <italic>Phaseolus vulgaris</italic> pathogen, <italic>Pseudomonas syringae</italic> pv. <italic>phaseolicola</italic> as well as human pathogenic bacteria (biocidal use). Our results, obtained with established methods, like Well diffusion and Disc diffusion assay, reveal that laurel essential oil is a very effective bacteriostatic and bactericidal agent. Importantly, the activity of laurel essential oil as growth inhibitor of the plant pathogen <italic>Pseudomonas syringae</italic> pv. <italic>phaseolicola</italic> is reported for the first time. This opens the field for more extended investigations regarding its use in crop protection. Additionally, the laurel essential oil tested showed significant antibacterial properties against several human pathogenic bacteria, namely <italic>Micrococcus luteus</italic>, <italic>Escherichia coli</italic>, <italic>Staphylococcus aureus</italic> and <italic>Bacillus subtilis</italic>.
</abstract>

Evaluation of the antibacterial activity of essential oil of <italic>Laurus nobilis</italic> against <italic>Pseudomonas syringae</italic> pv. <italic>phaseolicola</italic> and potential biocidal action

AHEAD OF PRINT

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Volume 16 (2023): Issue 1 (January 2023)

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Volume 14 (2021): Issue 2 (July 2021)

Volume 14 (2021): Issue 1 (January 2021)

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Volume 12 (2019): Issue 2 (July 2019)

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Volume 11 (2018): Issue 2 (July 2018)

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Hellenic Plant Protection Journal

 The Hellenic Plant Protection Journal (HPPJ) (ISSN 1791-3691) is the scientific publication of the Benaki Phytopathological Institute (BPI) replacing the Annals of the Benaki Phytopathological Institute (ISSN 1790-1480) which had been published since 1935. Starting from January 2008, the Hellenic Plant Protection Journal is published semiannually, in January and July each year.  HPPJ publishes scientific work on all aspects of plant health and plant protection referring to plant pathogens, pests, weeds, pesticides and relevant environmental and safety issues. In addition, the topics of the journal extend to aspects related to pests of public health in agricultural and urban areas.  Papers submitted for publication can be either in the form of a complete research article or in the form of a sufficiently documented short communication (including new records). Only original articles which have not been published or submitted for publication elsewhere are considered for publication in the journal. Review articles in related topics, either submitted or invited by the Editorial Board, are also published, normally one article per issue. Upon publication all articles are copyrighted by the BPI.  Manuscripts should be prepared according to instructions available to authors and submitted in electronic form through Editorial Manager. All submitted manuscripts are considered and published after successful completion of a review procedure by two competent referees.  The content of the articles published in HPPJ reflects the view and the official position of the authors. The information and opinions contained herein have not been adopted or approved by the HPPJ Editorial Board. The HPPJ Editorial Board neither guarantees the accuracy of the information included in the published articles nor may be held responsible for the use to which information contained herein may be put.  For all parties involved in the act of publishing (the author(s), the journal editor(s), the peer reviewers, and the publisher) it is necessary to agree upon standards of expected ethical behavior. HPPJ follows the ethics statements of De Gruyter journals, which are based on the Committee on Publication Ethics (COPE) Code of Conduct guidelines available at www.publicationethics.org.  Archiving  Sciendo archives the contents of this journal in Portico - digital long-term preservation service of scholarly books, journals and collections.  Plagiarism Policy  The editorial board is participating in a growing community of Similarity Check System's users in order to ensure that the content published is original and trustworthy. Similarity Check is a medium that allows for comprehensive manuscripts screening, aimed to eliminate plagiarism and provide a high standard and quality peer-review process. 

Hellenic Plant Protection Journal

Volume 16 (2023): Issue 1 (January 2023)

Evaluation of in-vitro antifungal activity against Fusarium incarnatum of binary and ternary combinations of lemongrass, garlic and mustard oil-encapsulated lipid nanoemulsions

Unraveling the role of endophytic fungi in barley salt-stress tolerance

First record of the scale insect Stotzia ephedrae in Greece

First record of the scale insect Lineaspis striata on Picea glauca “Conica”

Evaluation of the antibacterial activity of essential oil of Laurus nobilis against Pseudomonas syringae pv. phaseolicola and potential biocidal action

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