<abstract xmlns="http://www.w3.org/1999/xhtml">This study aims to develop a biocontrol agent against <italic>Fusarium oxysporum</italic> f.sp. <italic>radicis-lycopersic</italic>i (FORL) in tomato. For this, a set of 23 bacterial endophytic isolates has been screened for their ability to inhibit <italic>in vitro</italic> the growth of FORL using the dual plate assay. Three isolates with the most sound antagonistic activity to FORL have been qualitatively screened for siderophore production, phosphates solubilization and indolic acetic acid (IAA) synthesis as growth promotion traits. Antagonistic values of the three candidates against FORL were respectively: 51.51 % (EB4B), 51.18 % (EB22K) and 41.40 % (EB2A). Based on 16S rRNA gene sequence analysis, the isolates EB4B and EB22K were closely related to <italic>Enterobacter ludwigii</italic> EN-119, while the strain EB2A has been assigned to <italic>Leclercia adecarboxylata</italic> NBRC 102595. The promotion of tomato growth has been assessed <italic>in vitro</italic> using the strains EB2A, EB4B and EB22K in presence of the phytopathogen FORL. The treatments with the selected isolates increased significantly the root length and dry weight. Best results were observed in isolate EB4B in terms of growth promotion in the absence of FORL, improving 326.60 % of the root length and 142.70 % of plant dry weight if compared with untreated controls. In the presence of FORL, the strain EB4B improved both root length (180.81 %) and plant dry weight (202.15 %). These results encourage further characterization of the observed beneficial effect of <italic>Enterobacter</italic> sp. EB4B for a possible use as biofertilizer and biocontrol agent against FORL.</abstract>

Department of biology, Faculty of Nature and Life Sciences

Laboratoire de Biologie Moléculaire, Génomique et Bioinformatique (LBMGB)

Laboratoire de Biologie des Systèmes Microbiens (LBSM)

Effect of the endophytic plant growth promoting <italic>Enterobacter ludwigii</italic> EB4B on tomato growth

<abstract xmlns="http://www.w3.org/1999/xhtml">The olive fruit fly <italic>Bactrocera oleae</italic> (Rossi) (Diptera: Tephritidae) is the major pest of olives worldwide. The figitid wasp, <italic>Aganaspis daci</italic> (Hymenoptera: Figitidae), is a larval-prepupal endoparasitoid of fruit fly species, and it was found to successfully parasitize medfly larvae in field-infested figs in Greece. To assess the potential of <italic>A. daci</italic> as a biological control agent against <italic>B. oleae</italic>, we studied the effect of olive fruit size on parasitism rates of <italic>A. daci</italic> on 2nd and 3rd instar larvae of <italic>B. oleae</italic>, by using fruit of different size (cultivar ‘Chalkidikis’) and wild olive fruit. In addition, we conducted releases of <italic>A. daci</italic> females in a pilot olive grove in Volos, Magnesia. From July to October, we released 200 <italic>A. daci</italic> females/0.1 ha/week, followed by olive fruit sampling to estimate olive fruit infestation levels and the parasitism rates of <italic>A. daci</italic>. Laboratory trials revealed that fruit size and larvae instar were predictors of parasitism success of <italic>A. daci</italic>, with parasitism rates higher for small-size fruit of the cultivar “Chalkidikis” and the 3rd instar larvae of <italic>B. oleae</italic>. In field trials, no <italic>A. daci</italic> adults emerged from the olive fly infested fruit.</abstract>

Effect of the olive fruit size on the parasitism rates of <italic>Bactocera oleae</italic> (Diptera: Tephritidae) by the figitid wasp <italic>Aganaspis daci</italic> (Hymenoptera: Figitidae), and first field releases of adult parasitoids in olive grove

<abstract xmlns="http://www.w3.org/1999/xhtml">Root-knot nematodes (RKN), <italic>Meloidogyne</italic> spp., have a wide host range and are common in the Mediterranean area. Cultivated lavender (<italic>Lavandula angustifolia</italic>) was found naturally infested by <italic>M. hapla</italic> in Kozani area, the first documented infestation of this crop by RKN in Greece. <italic>Oxalis pescaprae</italic>, a common winter weed in Crete, was found to be a host of <italic>M. javanica</italic> under artificial inoculation. This weed acts as a potential winter host of the nematode in fields cultivated with vegetable crops. Two populations of <italic>M. ethiopica</italic> were found in kiwi and maize in Greece in the past. Recently, populations of <italic>M. ethiopica</italic> from Europe were re-classified as <italic>M. luci,</italic> based only on the population isolated from kiwi for Greece. In the current work, the RKN populations originating from kiwi and maize and maintained on tomato, were identified as <italic>M. luci.</italic> Nematode species identification was determined by electrophoretic analysis of protein extracts obtained from females.</abstract>

Department of Viticulture, Vegetable Crops, Flori-culture and Plant Protection, Institute of Olive Tree, Subtropical Crops and Viticulture, N.AG.RE.F., Hellenic Agricultural Organization-DEMETER

<italic>Lavandula angustifolia</italic> and <italic>Oxalis pes-caprae</italic>, hosts of <italic>Meloidogyne hapla</italic> and <italic>Meloidogyne javanica</italic> - A note for Meloidogyne luci in Greece

<abstract xmlns="http://www.w3.org/1999/xhtml">Enhancement of the resistance level in plants by rhizobacteria has been proven in several pathosystems. This study investigated the ability of four rhizobacteria strains (<italic>Pseudomonas putida</italic> BTP1 and <italic>Bacillus subtilis</italic> Bs2500, Bs2504 and Bs2508) to promote the growth in three barley genotypes and protect them against <italic>Cochliobolus sativus</italic>. Our results demonstrated that all tested rhizobacteria strains had a protective effect on barley genotypes Arabi Abiad, Banteng and WI2291. However, <italic>P. putida</italic> BTP1 and <italic>B. subtilis</italic> Bs2508 strains were the most effective as they reduced disease incidence by 53 and 38% (mean effect), respectively. On the other hand, there were significant differences among the rhizobacteria-treated genotypes on plant growth parameters, such as wet weight, dry weight, plant height and number of leaves. <italic>Pseudomonas putida</italic> BTP1 strain was the most effective as it significantly increased plant growth by 15-32%. In addition, the susceptible genotypes Arabi Abiad and WI2291 were the most responsive to rhizobacteria. This means that these genotypes have a high potential for increase of their resistance against the pathogen and enhancement of plant growth after the application of rhizobacteria. Consequently, barley seed treatment with the tested rhizobacteria could be considered as an effective biocontrol method against <italic>C. sativus</italic>.</abstract>

Effect of rhizobacteria strains on the induction of resistance in barley genotypes against <italic>Cochliobolus sativus</italic>

<abstract xmlns="http://www.w3.org/1999/xhtml">Phenology and parasitism of the scale insect, <italic>Coccus pseudomagnoliarum</italic> (Kuwana) (Hemiptera: Coccomorpha: Coccidae), infesting <italic>Citrus sinensis</italic> (Rutaceae), were studied in Papagou area, in northeastern Athens, from June 2015 to June 2017. <italic>Coccus pseudomagnoliarum</italic> is a univoltine, viviparous, parthenogenetic species. It overwintered as settled 1st instar nymph on the shoots of the trees. The 2nd instar nymphs appeared between the beginning of April and the end of May, and the mature females were recorded from the beginning of May until the middle of June. The crawlers appeared between the middle of May and the middle of June and the 1st instar nymphs settled on the shoots at the end of May, where they remained during the whole summer period, winter, until the beginning of April next year. Parasitism of the scale was recorded between the beginning of May and the middle of May and reached a maximum rate of 35%. The recorded parasitoid species were <italic>Coccophagus shillongensis</italic> Hayat and Singh (Hymenoptera: Aphelinidae), <italic>Coccophagus</italic> spp. and <italic>Metaphycus dispar</italic> (Mercet) (Hymenoptera: Encyrtidae).</abstract>

Department of Agriculture, School of Agriculture and Food

Department of Agriculture, School of Agriculture

The scale insect <italic>Coccus pseudomagnoliarum</italic> (Kuwana) (Hemiptera: Coccomorpha: Coccidae) on citrus in Greece

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Hellenic Plant Protection Journal

 The Hellenic Plant Protection Journal (HPPJ) (ISSN 1791-3691) is the scientific publication of the Benaki Phytopathological Institute (BPI) replacing the Annals of the Benaki Phytopathological Institute (ISSN 1790-1480) which had been published since 1935. Starting from January 2008, the Hellenic Plant Protection Journal is published semiannually, in January and July each year.  HPPJ publishes scientific work on all aspects of plant health and plant protection referring to plant pathogens, pests, weeds, pesticides and relevant environmental and safety issues. In addition, the topics of the journal extend to aspects related to pests of public health in agricultural and urban areas.  Papers submitted for publication can be either in the form of a complete research article or in the form of a sufficiently documented short communication (including new records). Only original articles which have not been published or submitted for publication elsewhere are considered for publication in the journal. Review articles in related topics, either submitted or invited by the Editorial Board, are also published, normally one article per issue. Upon publication all articles are copyrighted by the BPI.  Manuscripts should be prepared according to instructions available to authors and submitted in electronic form through Editorial Manager. All submitted manuscripts are considered and published after successful completion of a review procedure by two competent referees.  The content of the articles published in HPPJ reflects the view and the official position of the authors. The information and opinions contained herein have not been adopted or approved by the HPPJ Editorial Board. The HPPJ Editorial Board neither guarantees the accuracy of the information included in the published articles nor may be held responsible for the use to which information contained herein may be put.  For all parties involved in the act of publishing (the author(s), the journal editor(s), the peer reviewers, and the publisher) it is necessary to agree upon standards of expected ethical behavior. HPPJ follows the ethics statements of De Gruyter journals, which are based on the Committee on Publication Ethics (COPE) Code of Conduct guidelines available at www.publicationethics.org.  Archiving  Sciendo archives the contents of this journal in Portico - digital long-term preservation service of scholarly books, journals and collections.  Plagiarism Policy  The editorial board is participating in a growing community of Similarity Check System's users in order to ensure that the content published is original and trustworthy. Similarity Check is a medium that allows for comprehensive manuscripts screening, aimed to eliminate plagiarism and provide a high standard and quality peer-review process. 

Hellenic Plant Protection Journal

Volume 13 (2020): Issue 2 (July 2020)

Effect of the endophytic plant growth promoting Enterobacter ludwigii EB4B on tomato growth

Effect of the olive fruit size on the parasitism rates of Bactocera oleae (Diptera: Tephritidae) by the figitid wasp Aganaspis daci (Hymenoptera: Figitidae), and first field releases of adult parasitoids in olive grove

Lavandula angustifolia and Oxalis pes-caprae, hosts of Meloidogyne hapla and Meloidogyne javanica - A note for Meloidogyne luci in Greece

Effect of rhizobacteria strains on the induction of resistance in barley genotypes against Cochliobolus sativus

The scale insect Coccus pseudomagnoliarum (Kuwana) (Hemiptera: Coccomorpha: Coccidae) on citrus in Greece

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