<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Background</bold>: During the last two decades a major increase in the proportion of severe fungal infections has been noted due to the excessive use of broad-spectrum antibiotics, catheters, and a growing number of immunocompromised patients.<bold>Objectives</bold>: This is the first investigation providing complete data regarding the phenotypic and genotypic profiles of Candida albicans (C. albicans) isolates in Romanian patients.<bold>Methods</bold>: We investigated 301 isolates in terms of genotype determination (G), resistogram (R), phospholipase activity (Pl), haemolysis (Hl), proteinase activity (Pt), and biofilm formation (BF).<bold>Results</bold>: The analyzed isolates of C. albicans showed low values for Pt (61.73%), Hl (95.49%), and BF (60.71%), and did not present any Pl activity (92.23%). More than half of the investigated samples were genotype A with 450 bp (52.92%) and the majority (86.19%) were resistant to sodium selenite (A), boric acid (B), sodium periodate (D) and silver nitrate (E), but sensitive to cetrimide (-). One-way ANOVA analysis revealed significant effects of the infection site on biofilm formation (p = 0.0137) and no significant correlation was found between the genotype (A, B, C) and the infection site (p =0.449).<bold>Conclusions</bold>: Based on the obtained results it can be concluded that C. albicans isolates in Romanian patients exhibit different genotypic and phenotypic patterns, and no significant correlations between genotype and infection site could be observed.</abstract>

Background: During the last two decades a major increase in the proportion of severe fungal infections has been noted due to the excessive use of broad-spectrum antibiotics, catheters, and a growing number of immunocompromised patients.Objectives: This is the first investigation providing complete data regarding the phenotypic and genotypic profiles of Candida albicans (C. albicans) isolates in Romanian patients.Methods: We investigated 301 isolates in terms of genotype determination (G), resistogram (R), phospholipase activity (Pl), haemolysis (Hl), proteinase activity (Pt), and biofilm formation (BF).Results: The analyzed isolates of C. albicans showed low values for Pt (61.73%), Hl (95.49%), and BF (60.71%), and did not present any Pl activity (92.23%). More than half of the investigated samples were genotype A with 450 bp (52.92%) and the majority (86.19%) were resistant to sodium selenite (A), boric acid (B), sodium periodate (D) and silver nitrate (E), but sensitive to cetrimide (-). One-way ANOVA analysis revealed significant effects of the infection site on biofilm formation (p = 0.0137) and no significant correlation was found between the genotype (A, B, C) and the infection site (p =0.449).Conclusions: Based on the obtained results it can be concluded that C. albicans isolates in Romanian patients exhibit different genotypic and phenotypic patterns, and no significant correlations between genotype and infection site could be observed.

Phenotypic and genotypic variations in <italic>Candida albicans</italic> isolates from Romanian patients

Centre of Advanced Research in Bionanoconjugates and Biopolymers, Petru Poni Institute of Macromolecular Chemistry

Gr. T. Popa University of Medicine and Pharmacy

Matei Bals National Institute of Infectious Diseases

Carol Davila University of Medicine and Pharmacy

Revista Romana de Medicina de Laborator

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Phenotypic and genotypic variations in  isolates from Romanian patients

Background: During the last two decades a major increase in the proportion of severe fungal infections has been noted due to the excessive use of...

Phenotypic and genotypic variations in Candida albicans isolates from Romanian patients

Data publikacji: 29 paź 2018

Zakres stron: 405 - 413

Otrzymano: 07 maj 2018

Przyjęty: 21 cze 2018

DOI: https://doi.org/10.2478/rrlm-2018-0023

Słowa kluczowe
Candida albicans, infection site, genotype, phenotypes, Romania

© 2018 Irina Rosca et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Phenotypic and genotypic variations in Candida albicans isolates from Romanian patients

Data publikacji: 29 paź 2018

Zakres stron: 405 - 413

Otrzymano: 07 maj 2018

Przyjęty: 21 cze 2018

DOI: https://doi.org/10.2478/rrlm-2018-0023

Słowa kluczoweCandida albicans, infection site, genotype, phenotypes, Romania

© 2018 Irina Rosca et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Słowa kluczowe
Candida albicans, infection site, genotype, phenotypes, Romania