<abstract xmlns="http://www.w3.org/1999/xhtml"><sec id="j_1905-7415.0904.430_s_001_w2aab3b7ab1b6b1aab1c16b1Aa"><h3>Background</h3><p>Hyperlipidemia can induce the endogenous production of reactive oxygen species (ROS), which may cause carcinogenesis. Cytochrome P450 (CYP) 2E1 activity, induced by various factors including polyunsaturated fatty acids, effects the incidence of cancers, whereas NQO1, a flavoprotein, may protect against ROS.</p></sec><sec id="j_1905-7415.0904.430_s_002_w2aab3b7ab1b6b1aab1c16b2Aa"><h3>Objectives</h3><p>To investigate the effect of <italic>CYP2E1</italic> and <italic>NQO1</italic> polymorphism on oxidative stress status in Thais with and without dyslipidemia.</p></sec><sec id="j_1905-7415.0904.430_s_003_w2aab3b7ab1b6b1aab1c16b3Aa"><h3>Methods</h3><p>We included 1380 apparently healthy employees of the Electricity Generating Authority of Thailand in this study. We determined their <italic>CYP2E1</italic> and <italic>NQO1</italic> genotypes and related these to blood lipid profiles, and circulating levels of antioxidant enzymes, malondialdehyde (MDA), and reduced glutathione (GSH). Lifestyle-related factors were determined from questionnaires.</p></sec><sec id="j_1905-7415.0904.430_s_004_w2aab3b7ab1b6b1aab1c16b4Aa"><h3>Results</h3><p>All tested genotype frequencies were in Hardy-Weinberg equilibrium. The heterozygous and variant genotype distribution and allele frequency of <italic>CYP2E1 *5B</italic> were less common than <italic>CYP2E1 *6</italic>. Heterozygous <italic>NQO1</italic> was the most prevalent form. The frequency of the mutated allele <italic>CYP2E1 *5B</italic> was 0.16, <italic>CYP2E1 *6</italic> was 0.22, and <italic>NQO1 *2</italic> was 0.43. Significant differences were observed for blood cholesterol, triglyceride, low-density lipoprotein-cholesterol, and high-density lipoprotein-cholesterol between normolipidemic participants, and those with hypercholesterolemia, hypertriglyceridemia, and combined hyperlipidemia. Participants in the hyperlipidemic subgroup who bore any variant alleles of genes had higher plasma MDA and GSH levels, and superoxide dismutase and glutathione peroxidase activity, but lower catalase activity when compared with normolipidemic participants bearing wild-type alleles.</p></sec><sec id="j_1905-7415.0904.430_s_005_w2aab3b7ab1b6b1aab1c16b5Aa"><h3>Conclusions</h3><p>Variations in genetic disposition and dyslipidemia can modify oxidative stress status. Relatively more free radicals may be generated in individuals in subgroups with hyperlipidemia bearing any variant alleles.</p></sec></abstract>

BackgroundHyperlipidemia can induce the endogenous production of reactive oxygen species (ROS), which may cause carcinogenesis. Cytochrome P450 (CYP) 2E1 activity, induced by various factors including polyunsaturated fatty acids, effects the incidence of cancers, whereas NQO1, a flavoprotein, may protect against ROS.ObjectivesTo investigate the effect of CYP2E1 and NQO1 polymorphism on oxidative stress status in Thais with and without dyslipidemia.MethodsWe included 1380 apparently healthy employees of the Electricity Generating Authority of Thailand in this study. We determined their CYP2E1 and NQO1 genotypes and related these to blood lipid profiles, and circulating levels of antioxidant enzymes, malondialdehyde (MDA), and reduced glutathione (GSH). Lifestyle-related factors were determined from questionnaires.ResultsAll tested genotype frequencies were in Hardy-Weinberg equilibrium. The heterozygous and variant genotype distribution and allele frequency of CYP2E1 *5B were less common than CYP2E1 *6. Heterozygous NQO1 was the most prevalent form. The frequency of the mutated allele CYP2E1 *5B was 0.16, CYP2E1 *6 was 0.22, and NQO1 *2 was 0.43. Significant differences were observed for blood cholesterol, triglyceride, low-density lipoprotein-cholesterol, and high-density lipoprotein-cholesterol between normolipidemic participants, and those with hypercholesterolemia, hypertriglyceridemia, and combined hyperlipidemia. Participants in the hyperlipidemic subgroup who bore any variant alleles of genes had higher plasma MDA and GSH levels, and superoxide dismutase and glutathione peroxidase activity, but lower catalase activity when compared with normolipidemic participants bearing wild-type alleles.ConclusionsVariations in genetic disposition and dyslipidemia can modify oxidative stress status. Relatively more free radicals may be generated in individuals in subgroups with hyperlipidemia bearing any variant alleles.

Role of <italic>CYP2E1</italic> and <italic>NQO1</italic> polymorphisms in oxidative stress derived cancer in Thais with and without dyslipidemia

Asian Biomedicine

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Role of  and  polymorphisms in oxidative stress derived cancer in Thais with and without dyslipidemia

BackgroundHyperlipidemia can induce the endogenous production of reactive oxygen species (ROS), which may cause carcinogenesis. Cytochrome P450 (CYP) 2E1...

Role of CYP2E1 and NQO1 polymorphisms in oxidative stress derived cancer in Thais with and without dyslipidemia

Article Category: Original article

Pubblicato online: 31 gen 2017

Pagine: 601 - 611

DOI: https://doi.org/10.5372/1905-7415.0904.430

Parole chiave
lipid profile, oxidative stress, polymorphism

© 2015 Pharrunrat Tanaviyutpakdee, Krongtong Yoovathaworn, Jintana Sirivarasai, Suwannee Chanprasertyothin, Pachara Panpunuan, Krittaya Petchpoung, Aninthita Tatsaneeyapant, Thunyachai Sura, Sming Kaojarern, Piyamit Sritara

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Role of CYP2E1 and NQO1 polymorphisms in oxidative stress derived cancer in Thais with and without dyslipidemia

Article Category: Original article

Pubblicato online: 31 gen 2017

Pagine: 601 - 611

DOI: https://doi.org/10.5372/1905-7415.0904.430

Parole chiavelipid profile, oxidative stress, polymorphism

© 2015 Pharrunrat Tanaviyutpakdee, Krongtong Yoovathaworn, Jintana Sirivarasai, Suwannee Chanprasertyothin, Pachara Panpunuan, Krittaya Petchpoung, Aninthita Tatsaneeyapant, Thunyachai Sura, Sming Kaojarern, Piyamit Sritara

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Parole chiave
lipid profile, oxidative stress, polymorphism