<abstract xmlns="http://www.w3.org/1999/xhtml"><p> The aim of this research was to compare the efficiency of different murine lines for genotoxicity assays. Rats and mice of different murine lines were used. The spontaneous and induced indexes were evaluated according to alkaline comet assay of peripheral blood leukocytes, micronucleus and chromosomic aberration assay of bone marrow cell, and sperm head morphology assay. In most of the evaluated assays the line of Balb/c mice turned out to be the ideal biomodel, with less spontaneous indexes and high induced indexes to the mutagen used; allowing to detect in a narrow error margin those substances that are classified of very low genotoxicity. These results demonstrate that genetically the line of Balb/c mice in both sexes is more stable than the other ones evaluated. This suggests the use of the Balb/c line on <italic>in vivo </italic>genotoxicity assay will increase sensibility and robustness.</p></abstract>

The aim of this research was to compare the efficiency of different murine lines for genotoxicity assays. Rats and mice of different murine lines were used. The spontaneous and induced indexes were evaluated according to alkaline comet assay of peripheral blood leukocytes, micronucleus and chromosomic aberration assay of bone marrow cell, and sperm head morphology assay. In most of the evaluated assays the line of Balb/c mice turned out to be the ideal biomodel, with less spontaneous indexes and high induced indexes to the mutagen used; allowing to detect in a narrow error margin those substances that are classified of very low genotoxicity. These results demonstrate that genetically the line of Balb/c mice in both sexes is more stable than the other ones evaluated. This suggests the use of the Balb/c line on in vivo genotoxicity assay will increase sensibility and robustness.

Comparison in the efficiency of different murine lines for genotoxicity assays

Finlay Institute, Research Vicepresidency, 17 avenue e/ 198 and 200, Atabey, Playa, 16017, 11600, Havana, Cuba

Institute of Pharmacy and Food Science, Havana, Cuba

Agrarian University of Havana, Veterinary Faculty, Havana, Cuba.

University of Havana, Biology Faculty, Havana, Cuba.

Center of Study for the Research and Biological Evaluations (CEIEB-U.H), Havana, Cuba

Interdisciplinary Toxicology

{"article-title":"Comparison in the efficiency of different murine lines for genotoxicity assays"}

The aim of this research was to compare the efficiency of different murine lines for genotoxicity assays. Rats and mice of different murine lines were used....

Comparison in the efficiency of different murine lines for genotoxicity assays

Pubblicato online: 16 gen 2013

Pagine: 141 - 149

DOI: https://doi.org/10.2478/v10102-012-0023-4

This content is open access.