<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Background</bold>: Prunella vulgaris L., known as self-healing herb, is a widely spread species in the spontaneous flora with beneficial effects on human health, a fact proven in particular by Asian researchers. The aim of this study was to evaluate the antitumor activity and the antibacterial effect on different bacterial strains, including multidrug-resistant ones, depending on the type of solvent used (aqueous, hydroalcoholic), the plant product taken into consideration (spike inflorescence, leaf), its quantity and the concentration of active principles.<bold>Material and method</bold>: For screening of antimicrobial susceptibility, both minimum inhibitory concentration and minimum bactericidal concentration were determined on Escherichia coli, Klebsiella pneumoniae, Providencia stuartii, Pseudomonas aeruginosai, and methicillin-resistant Staphylococcus aureus species, including reference strains and hospital strains. Leaves and flower extracts (aqueous and 70% methanolic) were first assessed, and the one with the best antibacterial potential was further tested as a concentrated extract. The antitumor activity was determined on MDA-MB-231 breast adenocarcinoma cells and on a non-tumor cell line, MCF-10A breast epithelial cells by means of Alamar blue technique and Scratch assay.<bold>Results</bold>: Inflorescence extracts showed better bacteriostatic effects than leaf extracts on most bacteria, in both aqueous and hydroalcoholic extracts. The concentrated extract of spike inflorescence showed measurable activity with good effects on Gram-positive bacteria, but also on multidrug-resistant Gram-negative ones. The 70% methanolic extract of the species Prunella vulgaris L. (spike inflorescence) demonstrated a concentration-dependent cytotoxic and anti-migratory activity on MDA-MB-231 breast cancer cells, while affecting the non-tumor cell line less.<bold>Conclusions</bold>: The results suggest that Prunella vulgaris extracts present antibacterial potential in the complementary treatment of multidrug-resistant infections. The extract from the spike inflorescence of Prunella vulgaris L. produced a dose and time-dependent reduction in cell viability and migration, eliciting a stronger effect on the breast adenocarcinoma cell line.</abstract>

Background: Prunella vulgaris L., known as self-healing herb, is a widely spread species in the spontaneous flora with beneficial effects on human health, a fact proven in particular by Asian researchers. The aim of this study was to evaluate the antitumor activity and the antibacterial effect on different bacterial strains, including multidrug-resistant ones, depending on the type of solvent used (aqueous, hydroalcoholic), the plant product taken into consideration (spike inflorescence, leaf), its quantity and the concentration of active principles.Material and method: For screening of antimicrobial susceptibility, both minimum inhibitory concentration and minimum bactericidal concentration were determined on Escherichia coli, Klebsiella pneumoniae, Providencia stuartii, Pseudomonas aeruginosai, and methicillin-resistant Staphylococcus aureus species, including reference strains and hospital strains. Leaves and flower extracts (aqueous and 70% methanolic) were first assessed, and the one with the best antibacterial potential was further tested as a concentrated extract. The antitumor activity was determined on MDA-MB-231 breast adenocarcinoma cells and on a non-tumor cell line, MCF-10A breast epithelial cells by means of Alamar blue technique and Scratch assay.Results: Inflorescence extracts showed better bacteriostatic effects than leaf extracts on most bacteria, in both aqueous and hydroalcoholic extracts. The concentrated extract of spike inflorescence showed measurable activity with good effects on Gram-positive bacteria, but also on multidrug-resistant Gram-negative ones. The 70% methanolic extract of the species Prunella vulgaris L. (spike inflorescence) demonstrated a concentration-dependent cytotoxic and anti-migratory activity on MDA-MB-231 breast cancer cells, while affecting the non-tumor cell line less.Conclusions: The results suggest that Prunella vulgaris extracts present antibacterial potential in the complementary treatment of multidrug-resistant infections. The extract from the spike inflorescence of Prunella vulgaris L. produced a dose and time-dependent reduction in cell viability and migration, eliciting a stronger effect on the breast adenocarcinoma cell line.

Antibacterial and antitumor activity of the species <italic>Prunella vulgaris L</italic>.

Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy

George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Târgu Mureş

Department of Pharmacognosy and Phytotherapy, Faculty of Pharmacy

Victor Babeş University of Medicine and Pharmacy

Department of Microbiology, Faculty of Medicine

Department of Pharmaceutical Technology and Biopharmaceutics

Iuliu Haţieganu University of Medicine and Pharmacy

Department of Analytical Chemistry and Drug Analysis, Faculty of Pharmacy

Revista Romana de Medicina de Laborator

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Antibacterial and antitumor activity of the species .

Background: Prunella vulgaris L., known as self-healing herb, is a widely spread species in the spontaneous flora with beneficial effects on human health, a...

Antibacterial and antitumor activity of the species Prunella vulgaris L.

Pubblicato online: 18 nov 2020

Pagine: 405 - 417

Ricevuto: 20 mag 2020

Accettato: 21 lug 2020

DOI: https://doi.org/10.2478/rrlm-2020-0031

Parole chiave
Prunella vulgaris L., antibacterial, antitumor

© 2020 Alexandra Groşan et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Antibacterial and antitumor activity of the species Prunella vulgaris L.

Pubblicato online: 18 nov 2020

Pagine: 405 - 417

Ricevuto: 20 mag 2020

Accettato: 21 lug 2020

DOI: https://doi.org/10.2478/rrlm-2020-0031

Parole chiavePrunella vulgaris L., antibacterial, antitumor

© 2020 Alexandra Groşan et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Parole chiave
Prunella vulgaris L., antibacterial, antitumor