Establishment and application of a reverse dot blot assay for 13 mutations of hearing-loss genes in primary hospitals in China
, , , , , , y | 20 mar 2024
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Article Category: Original Article
Publicado en línea: 20 mar 2024
Páginas: 11 - 17
DOI: https://doi.org/10.2478/abm-2024-0003
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© 2024 Qing-Qing Jiang et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.
Figure 1.
Figure 2.
The information on primers in the reaction system
| GJB2-F | (Biotin)-TAGTGATTCCTGTGTTGTGTG |
| GJB2-R | TTCTGGGTTTTTGATCTCCTC |
| GJB3-F | (Biotin)-CTCTTCCTCTACCTGCTGC |
| GJB3-R | TATTGCCTGGGTCTGGAT |
| SLC26A4-F1 | (Biotin)-TTCACTGCTGGATTGCTCA |
| SLC26A4-R1 | GTGTTAACCGTACATGTTCTGC |
| SLC26A4-F2 | (Biotin)-CTCTCAGATGGTATGGCGTC |
| SLC26A4-R2 | TCCTTCATTACTGATTCCTTGTC |
| SLC26A4-F3 | (Biotin)-GTTCTTTGACGACAACATTAG |
| SLC26A3-R3 | AATGGAACCTTGACCCTCTT |
| mtDNA-F1 | (Biotin)-ATGAGGTGGCAAGAAATG |
| mtDNA-R1 | TTGGCTAAGGTTGTCTGGTA |
| mtDNA-F2 | (Biotin)-GATGCATACACCACATGAAAC |
| mtDNA-R2 | TGAGTGTTAGGAAAAGGGCA |
| mtDNA-F3 | (Biotin)-CACAATGGGGCTCACTCA |
| mtDNA-R3 | ACGAACAATGCTACAGGGAT |
The information of probes in the hearing loss gene chip
| 1494-PN | CGTCACCCTCCTCAAG |
| 1494-PM | CCGTCACTCTCCTCAAG |
| 1555 PN | AGAGGAGACAAGTCGTAAC |
| 1555 PM | GAGGAGGCAAGTCGTAA |
| 7445 PN | ACATAAAATCTAGA-CAAAAAAGGA |
| 7445 PM | CATAAAATCTAGGCAAAAAAGG |
| 12201 PN | GACCCCTTATTTACCGAGAA |
| 12201 PM | CCCCTTACTTACCGAGA |
| G2-35 PN | GTTCACACCCCCCAGGA |
| G2-35 PM | AATGGAACCTTGACCCTCTT |
| G2-155 PN | GGTGTTGCAGACAAAGTCG |
| G2-155 PM | CGACTTTGCAACACCC |
| G2-176 PN | GCACACGTTCTTGCAGCC |
| G2-176 PM | CAGCCAGCTACGATCAC |
| G2-235 PN | AGCTGCAGGGCCCATA |
| G2-235 PM | TATGGGCCTGCAGCT |
| G2-299 PN | TTCTTCTCATGTCTCCGGT |
| G2-299 PM | ACCGGAGACGAGAAGAA |
| G3-538 PN | ACATTGCCCGACCTAC |
| G3-538 PM | ACATTGCCTGACCTACC |
| IVS7-2 PN | GTTTTATTTCAGACGATAATTGC |
| IVS7-2 PM | TGTTTTATTTCGGACGATAAT |
| 1229 PN | CTGCTCTTTCCCGCACGG |
| 1229 PM | CTCTTTCCCGCATGGCC |
| 2168 PN | CGGTCCATGATGCTATA |
| 2168 PM | GTCCGTGATGCTATACT |
The genotypes of nine individuals with hearing loss gene mutation
| c.35delG | 0 | c.538C>T | 0 | IVS7-2A>G | 2 | m.1494C>T | 0 |
| c.155_158del4 | 0 | c.1229C>T | 1 | m.1555A>G | 1 | ||
| c.176_191del16 | 0 | c.2168A>G | 0 | m.7445A>G | 0 | ||
| c.235delC | 4 | m.12201T>C | 0 | ||||
| c.299_300delAT | 1 |