Establishment and application of a reverse dot blot assay for 13 mutations of hearing-loss genes in primary hospitals in China
Article Category: Original Article
Online veröffentlicht: 20. März 2024
Seitenbereich: 11 - 17
DOI: https://doi.org/10.2478/abm-2024-0003
Schlüsselwörter
© 2024 Qing-Qing Jiang et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.
Background
Hearing loss is a common sensorineural dysfunction with a high incidence in China. Although genetic factors are important causes of hearing loss, hearing-related gene detection has not been widely adopted in China.
Objective
Establishing a rapid and efficient method to simultaneously detect hotspot hearing loss gene mutations.
Methods
A reverse dot blot assay combined with a flow-through hybridization technique was developed for the simultaneous detection of 13 hotspot mutations of 4 hearing loss–related genes including
Results
The technique can detect 13 hotspot mutations of 4 hearing loss–related genes. And a total of 213 blood samples were used to evaluate the availability of this method.
Discussion
Our reverse dot blot assay was a simple, rapid, accurate, and cost-effective method to identify hotspot mutations of 4 hearing loss–related genes in a Chinese population.