<abstract xmlns="http://www.w3.org/1999/xhtml"> Background: Naegleria fowleri, a free-living amoeboflagellate, is the causative agent of life threatening primary amoebic meningoencephalitis with up to a 98% mortality rate. The pathogenesis involves neuronal dysfunction and damages the host central nervous system. Understanding the interactions of the target cells and the protozoa may facilitate an integrated approach to address important biological questions.Objective: We investigated changes in protein expression of the host cells post Naegleria fowleri infection.Methods: The analysis was based on two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) of the proteins extracted from human neuroblastoma SK-N-MC after exposure to N. fowleri trophozoites over a period of six hours. We observed differences in protein expression as early as 15 minutes after the exposure, and the differences remained throughout the six hours study.Results: The identified protein spots corresponded to 53 proteins unique to human and 24 of these proteins were identified as involved in signal transduction, translation, immune response, cytoskeleton, and stress response. We also observed changes in the abundance of these proteins after cocultivating SK-N-MC with N. fowleri, especially those involved in angiogenesis, fatty acid metabolism, signal transduction, translation, stress response, signal pathway, and respiration.Conclusions: The proteomics analysis from this report suggested how and by which means the SK-N-MC responded after N. fowleri infection.</abstract>

Background: Naegleria fowleri, a free-living amoeboflagellate, is the causative agent of life threatening primary amoebic meningoencephalitis with up to a 98% mortality rate. The pathogenesis involves neuronal dysfunction and damages the host central nervous system. Understanding the interactions of the target cells and the protozoa may facilitate an integrated approach to address important biological questions.Objective: We investigated changes in protein expression of the host cells post Naegleria fowleri infection.Methods: The analysis was based on two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) of the proteins extracted from human neuroblastoma SK-N-MC after exposure to N. fowleri trophozoites over a period of six hours. We observed differences in protein expression as early as 15 minutes after the exposure, and the differences remained throughout the six hours study.Results: The identified protein spots corresponded to 53 proteins unique to human and 24 of these proteins were identified as involved in signal transduction, translation, immune response, cytoskeleton, and stress response. We also observed changes in the abundance of these proteins after cocultivating SK-N-MC with N. fowleri, especially those involved in angiogenesis, fatty acid metabolism, signal transduction, translation, stress response, signal pathway, and respiration.Conclusions: The proteomics analysis from this report suggested how and by which means the SK-N-MC responded after N. fowleri infection.

Original article. Expression profile of human neuroblastoma cells after exposure to Naegleria fowleri

Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, 10700 Bangkok,

Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, 10700 Bangkok,

Department of Biology, Faculty of Science, Silpakorn University, Nakhon Pathom 73000,

Genome Institute, National Center for Genetic Engineering and Biotechnology, Pathumthani 12120,

Department of Biology, Faculty of Science, Mahidol University, Bangkok 10400,Mahidol University, Nakhon Pathom 73170,

Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom 73170,

Asian Biomedicine

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Background: Naegleria fowleri, a free-living amoeboflagellate, is the causative agent of life threatening primary amoebic meningoencephalitis with up to a 98%...

Original article. Expression profile of human neuroblastoma cells after exposure to Naegleria fowleri

Published Online: Feb 04, 2017

Page range: 211 - 218

DOI: https://doi.org/10.5372/1905-7415.0702.168

Keywords
Host response trophozoites, human neuroblastoma SK-N-MC, Naegleria fowleri infection proteomics

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Original article. Expression profile of human neuroblastoma cells after exposure to Naegleria fowleri

Published Online: Feb 04, 2017

Page range: 211 - 218

DOI: https://doi.org/10.5372/1905-7415.0702.168

KeywordsHost response trophozoites, human neuroblastoma SK-N-MC, Naegleria fowleri infection proteomics

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
Host response trophozoites, human neuroblastoma SK-N-MC, Naegleria fowleri infection proteomics