<abstract xmlns="http://www.w3.org/1999/xhtml">

<p> The aim of the study was to define morphology and distribution of calretinin (CR) positive neurons in the frontal cortex of adult chinchilla males and intracellular localisation of the protein in this area. The brains of 5 adult chinchilla males were used in the study. CR immunoreactive neurons were shown with peroxidase-antiperoxidase immunohistochemical reaction using a specific monoclonal antibody. Intensive CR immunoreactivity was demonstrated mainly in few polymorphic neurons of II, III, and V layers. Cytoplasmic and nuclear reaction product in most CR positive neurons was diffuse and in some neurons of layer V in the form of granules localised peripherally. In a few cells more intensive staining was observed in the nuclei than in the cytoplasm. The results indicate the presence of heteromorphic CR positive neurons in specific layers of the frontal cortex. Nuclear localisation of CR in neurons suggests passive transport of this protein, which may affect the nuclear genes. This protein is a neuroprotector maintaining appropriate level of calcium, modulating neuronal activity, and synaptic conduction in the frontal cortex of chinchilla.</p>
</abstract>

The aim of the study was to define morphology and distribution of calretinin (CR) positive neurons in the frontal cortex of adult chinchilla males and intracellular localisation of the protein in this area. The brains of 5 adult chinchilla males were used in the study. CR immunoreactive neurons were shown with peroxidase-antiperoxidase immunohistochemical reaction using a specific monoclonal antibody. Intensive CR immunoreactivity was demonstrated mainly in few polymorphic neurons of II, III, and V layers. Cytoplasmic and nuclear reaction product in most CR positive neurons was diffuse and in some neurons of layer V in the form of granules localised peripherally. In a few cells more intensive staining was observed in the nuclei than in the cytoplasm. The results indicate the presence of heteromorphic CR positive neurons in specific layers of the frontal cortex. Nuclear localisation of CR in neurons suggests passive transport of this protein, which may affect the nuclear genes. This protein is a neuroprotector maintaining appropriate level of calcium, modulating neuronal activity, and synaptic conduction in the frontal cortex of chinchilla.

Immunocytochemical Analysis of Calretinin in the Frontal Cortex of Chinchilla

Department of Animal Anatomy and Histology, Faculty of Veterinary Medicine

Journal of Veterinary Research

{"article-title":"Immunocytochemical Analysis of Calretinin in the Frontal Cortex of Chinchilla"}

The aim of the study was to define morphology and distribution of calretinin (CR) positive neurons in the frontal cortex of adult chinchilla males and...

Immunocytochemical Analysis of Calretinin in the Frontal Cortex of Chinchilla

Published Online: Jan 17, 2013

Page range: 103 - 107

DOI: https://doi.org/10.2478/v10213-012-0019-z

This content is open access.