1. bookVolume 50 (2013): Issue 2 (June 2013)
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First Published
22 Apr 2006
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English
access type Open Access

Effect of low temperatures on the infectivity of Toxocara cati larvae parasitized in mouse tissues

Published Online: 31 Aug 2013
Page range: 83 - 86
Journal Details
License
Format
Journal
First Published
22 Apr 2006
Publication timeframe
4 times per year
Languages
English

The infectivity of Toxocara cati larvae in mouse tissues, following storage at 4 °C and −25 °C, was measured using a bioassay in mice. Tissues of donor mice infected with 30-day-old T. cati larvae were stored at 4 °C for 7 and 14 days, and at −25 °C for 24 hours, then tissue larvae were inoculated into recipient mice (n = 6/group). After 15 days, larval burden in recipient mice was assessed by digestion. In the control group, a mean of 42.0 % of the inoculated larvae were established in mice. Storage of tissues at 4 °C for 7 and 14 days did not affect larval infectivity; means of 43.5 % and 37.4 % of inoculated larvae, respectively, were established in mice. Larvae exposed to −25 °C for 24 hours did not establish in mice, except for one larva recovered from a single mouse. These results may support the potential of tissue larvae in livestock animals as a causative agent of human toxocarosis.

Keywords

[1] Akao, N., Ohta, N. (2007): Toxocariasis in Japan. Parasitol. Int., 56: 87–93. DOI: 10.1016/j.parint.2007.01.009 http://dx.doi.org/10.1016/j.parint.2007.01.009Search in Google Scholar

[2] Fisher, M. (2003) Toxocara cati: An underestimated zoonotic agent. Trends Parasitol., 19: 167–170. DOI: 10.1016/S1471-4922(03)00027-8 http://dx.doi.org/10.1016/S1471-4922(03)00027-8Search in Google Scholar

[3] Lee, A. C., Schantz, P. M., Kazacos, K. R., Montgomery, S. P., Bowman, D. D. (2010): Epidemiologic and zoonotic aspects of ascarid infections in dogs and cats. Trends Parasitol., 26: 155–161. DOI: 10.1016/j.pt.2010.01.002 http://dx.doi.org/10.1016/j.pt.2010.01.002Search in Google Scholar

[4] Mitsugi, K., Umei, T., Inoue, T., Sumida, I., Hanada, M. (1988): Visceral larva migrans by Toxocara cati with multiple nodules in liver. J. Nihon Naika Gakkai, 77: 1742–1743 http://dx.doi.org/10.2169/naika.77.1742Search in Google Scholar

[5] Nishikata, H., Hirata, Y., Shimamura, R., Dohmen, K., Kudo, J., Ishibashi, H., Onizuka, H., Oda, M. (1991): A case of visceral larva migrans by Toxocara cati infection with multiple liver granuloma. Nihon Shokakibyo Gakkai Zasshi, 88: 2697–2702 Search in Google Scholar

[6] Okulewicz, A., Perec-matysiak, A., Buńkowska, K., Hildebrand, J. (2012): Toxocara canis, Toxocara cati and Toxascaris leonina in wild and domestic carnivores. Helminthologia, 49: 3–10, DOI: 10.2478/s11687-012-0001-6 http://dx.doi.org/10.2478/s11687-012-0001-6Search in Google Scholar

[7] Taira, K., Saeed, I., Permin, A., Kapel, C. M. (2004): Zoonotic risk of Toxocara canis infection through consumption of pig or poultry viscera. Vet. Parasitol., 121: 115–124. DOI: 10.1016/j.vetpar.2004.01.018 http://dx.doi.org/10.1016/j.vetpar.2004.01.018Search in Google Scholar

[8] Taira, K., Saitoh, Y., Kapel, C. M. (2011): Toxocara cati larvae persist and retain high infectivity in muscles of experimentally infected chickens. Vet. Parasitol., 180: 287–291. DOI: 10.1016/j.vetpar.2011.03.020 http://dx.doi.org/10.1016/j.vetpar.2011.03.020Search in Google Scholar

[9] Taira, K., Saitoh, Y., Okada, N., Sugiyama, H., Kapel, C. M. (2012): Tolerance to low temperatures of Toxocara cati larvae in chicken muscle tissue. Vet. Parasitol., 189: 383–386. DOI: 10.1016/j.vetpar.2012.04.037 http://dx.doi.org/10.1016/j.vetpar.2012.04.037Search in Google Scholar

[10] Yoshikawa, M., Koyama, N., Hontsu, S., Yamamoto, Y., Ogawa, S., Nakamura, T., Mizuno, Y., Miura, S., Mikasa, K., Kimura, H. (2010): Clinical analysis of eight patients with pulmonary toxocariasis. J. Jpn. Resp. Soc., 8: 351–356 Search in Google Scholar

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