1. bookVolume 48 (2011): Issue 1 (March 2011)
Journal Details
License
Format
Journal
eISSN
1336-9083
ISSN
0440-6605
First Published
22 Apr 2006
Publication timeframe
4 times per year
Languages
English
access type Open Access

Molecular differentiation of Turkey cattle isolates of Fasciola hepatica and Fasciola gigantica

Published Online: 11 Mar 2011
Volume & Issue: Volume 48 (2011) - Issue 1 (March 2011)
Page range: 3 - 7
Journal Details
License
Format
Journal
eISSN
1336-9083
ISSN
0440-6605
First Published
22 Apr 2006
Publication timeframe
4 times per year
Languages
English
Abstract

The most common and widespread liver flukes of the genus Fasciola are Fasciola hepatica and F. gigantica. Adults of both species occur in many domestic ruminants and in humans and can cause serious disease. The differential diagnosis of these flukes infection is very important because of their different transmission and epidemiological characteristics. A simple and rapid PCR-restriction fragment length polymorphism (RFLP) assay, using the common restriction enzymes AluI and RsaI, is described to distinguish between both fasciolid species. After the digestion of the mitochondrial cytochrome c oxidase 1 (CO1) PCR product with the restriction enzyme AluI, the RFLP profile obtained from F. hepatica revealed two fragments, whereas F. gigantica was not cut. The RsaI digestion generated two fragments from F. gigantica, whereas it did not cut the PCR product from F. hepatica. Results were confirmed with CO1 sequence analysis of both F. hepatica and F. gigantica. The present study suggests that the PCRRFLP method described here can be used for the proper identification of Fasciola species.

Keywords

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