1. bookVolume 28 (2020): Issue 4 (October 2020)
Journal Details
License
Format
Journal
eISSN
2284-5623
First Published
08 Aug 2013
Publication timeframe
4 times per year
Languages
English
access type Open Access

Antibacterial and antitumor activity of the species Prunella vulgaris L.

Published Online: 18 Nov 2020
Volume & Issue: Volume 28 (2020) - Issue 4 (October 2020)
Page range: 405 - 417
Received: 20 May 2020
Accepted: 21 Jul 2020
Journal Details
License
Format
Journal
eISSN
2284-5623
First Published
08 Aug 2013
Publication timeframe
4 times per year
Languages
English
Abstract

Background: Prunella vulgaris L., known as self-healing herb, is a widely spread species in the spontaneous flora with beneficial effects on human health, a fact proven in particular by Asian researchers. The aim of this study was to evaluate the antitumor activity and the antibacterial effect on different bacterial strains, including multidrug-resistant ones, depending on the type of solvent used (aqueous, hydroalcoholic), the plant product taken into consideration (spike inflorescence, leaf), its quantity and the concentration of active principles.

Material and method: For screening of antimicrobial susceptibility, both minimum inhibitory concentration and minimum bactericidal concentration were determined on Escherichia coli, Klebsiella pneumoniae, Providencia stuartii, Pseudomonas aeruginosai, and methicillin-resistant Staphylococcus aureus species, including reference strains and hospital strains. Leaves and flower extracts (aqueous and 70% methanolic) were first assessed, and the one with the best antibacterial potential was further tested as a concentrated extract. The antitumor activity was determined on MDA-MB-231 breast adenocarcinoma cells and on a non-tumor cell line, MCF-10A breast epithelial cells by means of Alamar blue technique and Scratch assay.

Results: Inflorescence extracts showed better bacteriostatic effects than leaf extracts on most bacteria, in both aqueous and hydroalcoholic extracts. The concentrated extract of spike inflorescence showed measurable activity with good effects on Gram-positive bacteria, but also on multidrug-resistant Gram-negative ones. The 70% methanolic extract of the species Prunella vulgaris L. (spike inflorescence) demonstrated a concentration-dependent cytotoxic and anti-migratory activity on MDA-MB-231 breast cancer cells, while affecting the non-tumor cell line less.

Conclusions: The results suggest that Prunella vulgaris extracts present antibacterial potential in the complementary treatment of multidrug-resistant infections. The extract from the spike inflorescence of Prunella vulgaris L. produced a dose and time-dependent reduction in cell viability and migration, eliciting a stronger effect on the breast adenocarcinoma cell line.

Keywords

1. Pinkas M, Trotin F, Peng M, Torck M. Use, chemistry and pharmacology of the Chinese medicinal plants. Fitotherapia. 1994. 55:343-53.Search in Google Scholar

2. Wagner H, Bauer R, Melchart D, Staudinger A, Spica prunellae, Xiacukao in Chromatographic Fingerprint Analysis of Herbal Medicines, Thin Layer and High Performance Liquid Chromatography of Chinese Drugs. Vol. 4, Springer International Publishing Switzerland 2016, pp. 225-237; ISBN 978-3-319-32328-2. DOI: 10.1007/978-3-319-32328-2_2210.1007/978-3-319-32328-2_22Search in Google Scholar

3. Duke J. Prunella vulgaris. Handbook of Edible Weeds, 2001, CRC, p.158.Search in Google Scholar

4. Gao W, Xu HLYLYLY. Root extract of Prunella vulgar-is inhibits in vitro and in vivo carcinogenesis in MCF-5 human breast carcinoma via suppression of angiogenesis, induction of apoptosis, cell cycle arrest and modulation of PI3K/AKT signalling pathway. J Buon. 2019 Mar-Apr; 24(2):549-554.Search in Google Scholar

5. Su YC, Lin IH, Siao YM, Liu CJ, Yeh CC. Modulation of the Tumor Metastatic Microenvironment and Multiple Signal Pathways by Prunella vulgaris in Human Hepatocellular Carcinoma. Am J Chin Med. 2016;44(4):835-49. DOI: 10.1142/S0192415X1650046410.1142/S0192415X1650046427222069Search in Google Scholar

6. Rasool R, Ganai BA, Kamili AN, Akbar S, Masodd A. Antioxidant and Antibacterial Activities of Extracts from Wild and in Vitro- Raised Cultures of Prunella Vulgaris L., Medicinal and aromatic plant science and biotechnology. 2010;4(1):20-7.Search in Google Scholar

7. Mahboubi M., Mahboubi A., Kazempour N., The antimicrobial activity of Prunella vulgaris extracts. Herb Pol. 2015;61(1):31-8. DOI: 10.1515/hepo-2015-000810.1515/hepo-2015-0008Search in Google Scholar

8. Psotová J, Kolár M, Sousek J, Svagera Z, Vicar J, Ulrichová J. Biological Activities of Prunella vulgaris Extract. Phytother Res. 2003 Nov;17(9):1082-7. DOI: 10.1002/ptr.132410.1002/ptr.132414595592Search in Google Scholar

9. Komal S, Kazmi SAJ, Khan JA, Gilani MM. Antimicrobial activity of Prunella Vulgaris extracts against multi-drug resistant Escherichia Coli from patients of urinary tract infection. Pak J Med Sci. 2018 May-Jun;34(3):616-20. DOI: 10.12669/pjms.343.1498210.12669/pjms.343.14982604153030034426Search in Google Scholar

10. Roh KB, Park D, Jung E. Inhibitory Effects of Prunella vulgaris L. Extract on 11β-HSD1 in Human Skin Cells. Evid Based Complement Alternat Med. 2018 Oct 4;2018:1762478 DOI: 10.1155/2018/176247810.1155/2018/1762478619334830402116Search in Google Scholar

11. Xu Y, Jiang Z, Ji G, Liu J. Inhibition of bone metastasis from breast carcinoma by rosmarinic acid. Planta Med. 2010 Jul;76(10):956-62. DOI: 10.1055/s-0029-124089310.1055/s-0029-124089320157877Search in Google Scholar

12. Benedec D, Hanganu D, Oniga I, Tiperciuc B, Olah NK, Raita O, et al. Assessment of rosmarinic acid content in six Lamiaceae species extracts and their antioxidant and antimicrobial potential. Pak J Pharm Sci. 2015 Nov;28(6 Suppl):2297-303.Search in Google Scholar

13. Toiu A, Vlase L, Vodnar DC, Gheldiu AM, Oniga I. Solidago graminifolia L. Salisb. (Asteraceae) as a valuable source of bioactive polyphenols: HPLC profile, in vitro antioxidant and antimicrobial potential. Molecules. 2019 Jul 23;24(14). pii: E2666. DOI: 10.3390/molecules2414266610.3390/molecules24142666668099731340530Search in Google Scholar

14. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically, 11th Edition. 2018 https://clsi.org/standards/products/microbiology/documents/m07Search in Google Scholar

15. Moacă EA, Pavel IZ, Danciu C, Crăiniceanu Z, Minda D, Ardelean F, et al. Romanian Wormwood (Artemis-ia absinthium L.): Physicochemical and Nutraceutical Screening. Molecules. 2019 Aug 25;24(17). pii: E3087. DOI: 10.3390/molecules2417308710.3390/molecules24173087674951731450704Search in Google Scholar

16. Danciu C, Muntean D, Alexa E, Farcas C, Oprean C Zupko I, et al. Phytochemical Characterization and Evaluation of the Antimicrobial, Antiproliferative and Pro-Apoptotic Potential of Ephedra alata Decne. Hydroalcoholic Extract against the MCF-7 Breast Cancer Cell Line. Molecules. 2018 Dec 20;24(1). pii: E13. DOI: 10.3390/molecules2401001310.3390/molecules24010013633752630577537Search in Google Scholar

17. Man A, Santacroce L, Iacob R, Mare AD, Man L. Antimicrobial activity of six essential oils against a group of human pathogens: A comparative study. Pathogens. 2019 Jan 28;8(1). pii: E15. DOI: 10.3390/pathogens801001510.3390/pathogens8010015647118030696051Search in Google Scholar

18. Grama L, Man A, Muntean DL, Gâz Florea ŞA, Boda F, Curticăpean A. Antibacterial activity of some saturated polyoxotungstates. Rev Romana Med Lab. 2014;22(1):111-8. DOI: 10.2478/rrlm-2014-000710.2478/rrlm-2014-0007Search in Google Scholar

19. Zhu J, Zhang W, Zhang Y, Wang Y, Liu M, Liu Y. Effects of Spica prunellae on caspase-3-associated proliferation and apoptosis in human lung cancer cells in vitro. J Cancer Res Ther. 2018;14(4):760-3. DOI: 10.4103/jcrt.JCRT_1289_1610.4103/jcrt.JCRT_1289_1629970649Search in Google Scholar

20. Hwang YJ, Lee EJ, Kim HR, Hwang KA. In vitro antioxidant and anticancer effects of solvent fractions from prunella vulgaris var. lilacina. BMC Complement Altern Med. 2013 Nov 9;13:310. DOI: 10.1186/1472-6882-13-31010.1186/1472-6882-13-310422620124206840Search in Google Scholar

21. Qiang Z, Ye Z, Hauck C, Murphy PA, McCoy JA, Widrlechner MP, Reddy MB, Hendrich S. Permeability of rosmarinic acid in Prunella vulgaris and ursolic acid in Salvia officinalis extracts across Caco-2 cell mono-layers, J Ethnopharmacol. 2011 Oct 11;137(3):1107-12. DOI: 10.1016/j.jep.2011.07.03710.1016/j.jep.2011.07.037320202921798330Search in Google Scholar

22. Feng L, Jia XB, Jiang J, Zhu MM, Chen Y, Tan XB, Shi F. Combination of active components enhances the efficacy of Prunella in prevention and treatment of lung cancer Molecules. 2010 Nov 4;15(11):7893-906. DOI: 10.3390/molecules1511789310.3390/molecules15117893625919021060297Search in Google Scholar

23. Su Y, Lin IH, Siao YM, Liu CJ, Yeh CC. Modulation of the Tumor Metastatic Microenvironment and Multiple Signal Pathways by Prunella vulgaris in Human Hepatocellular Carcinoma. Am J Chin Med. 2016;44(4):835-49. DOI: 10.1142/S0192415X1650046410.1142/S0192415X1650046427222069Search in Google Scholar

24. Feng L, Au-Yeung W, Xu YH, Wang SS, Zhu Q, Xiang P. Oleanolic acid from Prunella Vulgaris L. induces SPC-A-1 cell line apoptosis via regulation of Bax, Bad and Bcl-2 expression. Asian Pac J Cancer Prev. 2011;12(2):403-8.Search in Google Scholar

25. Mohsen SM, Ammar AS. Total phenolic contents and antioxidant activity of corn tassel extracts. Food Chem. 2009;112(3):595-8. DOI: 10.1016/j.food-chem.2008.06.014Search in Google Scholar

26. Feng L, Jia X, Zhu MM, Chen Y, Shi F. Antioxidant Activities of Total Phenols of Prunella vulgaris L. in Vitro and in Tumor-bearing Mice. Molecules. 2010 Dec 10;15(12):9145-56. DOI: 10.3390/molecules1512914510.3390/molecules15129145625916721150830Search in Google Scholar

27. Man A, Gâz Florea ŞA, Mare AD, Berta L. Effects of low-molecular weight alcohols on bacterial viability. Rev Romana Med Lab. 2017;25(4):335-43. DOI: 10.1515/rrlm-2017-002810.1515/rrlm-2017-0028Search in Google Scholar

28. Chen Y, Zhu Z, Guo Q, Zhang L, Zhang X. Variation in concentrations of major bioactive compounds in Prunella vulgaris L. related to plant parts and pheno-logical stages. Biological Research. 2012;45(2):171-5. DOI: 10.4067/S0716-9760201200020000910.4067/S0716-9760201200020000923096361Search in Google Scholar

29. Mahboubi M, Mahboubi A, Kazempour N. The antimicrobial activity of Prunella vulgaris extracts. Herba Polonica. 2015 Mar 1;61(1):31-8. DOI: 10.1515/hepo-2015-000810.1515/hepo-2015-0008Search in Google Scholar

30. Komal S, Kazmi SAJ, Khan JA, Gilani MM. Antimicrobial activity of Prunella Vulgaris extracts against multi-drug resistant Escherichia Coli from patients of urinary tract infection. Pak J Med Sci. 2018;34(3):616-20. DOI: 10.12669/pjms.343.1498210.12669/pjms.343.14982604153030034426Search in Google Scholar

31. Xie Y, Yang W, Tang F, Chen X, Ren L. Antibacterial activities of flavonoids: structure-activity relationship and mechanism. Curr Med Chem. 2015;22(1):132-49. DOI: 10.2174/092986732166614091611344310.2174/092986732166614091611344325245513Search in Google Scholar

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