Analysis of damage-associated molecular pattern molecules due to electroporation of cells in vitro
Article Category: Research Article
Published Online: Jul 29, 2020
Page range: 317 - 328
Received: Jun 18, 2020
Accepted: Jul 07, 2020
DOI: https://doi.org/10.2478/raon-2020-0047
© 2020 Tamara Polajzer, Tomaz Jarm, Damijan Miklavcic, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Background
Tumor cells can die via immunogenic cell death pathway, in which damage-associated molecular pattern molecules (DAMPs) are released from the cells. These molecules activate cells involved in the immune response. Both innate and adaptive immune response can be activated, causing a destruction of the remaining infected cells. Activation of immune response is also an important component of tumor treatment with electrochemotherapy (ECT) and irreversible electroporation (IRE). We thus explored, if and when specific DAMPs are released as a consequence of electroporation
Materials and methods
In this
Results
In general, the release of DAMPs increases with increasing pulse amplitude. Concentration of DAMPs depend on the time interval between exposure of the cells to pulses and the analysis. Concentrations of most DAMPs correlate strongly with cell death. However, we detected no uric acid in the investigated samples.
Conclusions
Release of DAMPs can serve as a marker for prediction of cell death. Since the stability of certain DAMPs is time dependent, this should be considered when designing protocols for detecting DAMPs after electric pulse treatment.