1. bookVolume 72 (2018): Issue 4 (August 2018)
Journal Details
License
Format
Journal
eISSN
2255-890X
First Published
14 Sep 2008
Publication timeframe
6 times per year
Languages
English
access type Open Access

Evaluation of “Stress Relief” Dietary Supplement on Animal Stress Level and Locomotion

Published Online: 22 Aug 2018
Volume & Issue: Volume 72 (2018) - Issue 4 (August 2018)
Page range: 216 - 229
Received: 30 Aug 2017
Accepted: 16 Feb 2018
Journal Details
License
Format
Journal
eISSN
2255-890X
First Published
14 Sep 2008
Publication timeframe
6 times per year
Languages
English
Abstract

Search of new approaches for harmless, non-medication treatment of body dysfunctions is still on the agenda of vet and human practitioners and researchers as well. This study presents evaluation of the effect of “Stress Relief” dietary supplement (SR) on mice behaviour under different acute short-term stress conditions and treatment duration. Five experiments were performed and in each 40 animals were randomly split into four (I–IV) groups, where I and II — non-stressed mice, III and IV — stressed animals, I and III received water with trace mineral solution (TMS), II and IV received water with SR. As stress factors, forced swimming, rodent predator odour or both together were applied. Locomotor activity under normal and stress conditions in Open Field were observed and measured by a SMART video-tracking system. Blood glucose level was measured as well. SR showed a reversal of stress-decreased locomotor activity in all stress models — distance walked increased almost twice (p < 0.0001), central zone crossings and time spent in it were 2–4 times greater than in the control group (p < 0.0001 and p = 0.0002, respectively), and fast movement episodes and maximal speed increased by 50–200%. In addition, complete normalisation of stress-induced elevation of blood glucose level (p < 0.0001) was noted. These results demonstrate for the first time that the effect of “Stress Relief” formula (water additives–minerals processed by know-how way in Vital Force Technology using Dr. Yury Kronn method) can be observed in laboratory animals, and that the effects are significant and repeatable. SR shows fear- and stress-reducing activity, which does not sufficiently differ between 7-, 9-, 14-, 28- and 32-day treatment regimes.

Keywords

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