1. bookVolume 72 (2018): Issue 4 (August 2018)
Journal Details
First Published
14 Sep 2008
Publication timeframe
6 times per year
access type Open Access

Evaluation of “Stress Relief” Dietary Supplement on Animal Stress Level and Locomotion

Published Online: 22 Aug 2018
Volume & Issue: Volume 72 (2018) - Issue 4 (August 2018)
Page range: 216 - 229
Received: 30 Aug 2017
Accepted: 16 Feb 2018
Journal Details
First Published
14 Sep 2008
Publication timeframe
6 times per year

Search of new approaches for harmless, non-medication treatment of body dysfunctions is still on the agenda of vet and human practitioners and researchers as well. This study presents evaluation of the effect of “Stress Relief” dietary supplement (SR) on mice behaviour under different acute short-term stress conditions and treatment duration. Five experiments were performed and in each 40 animals were randomly split into four (I–IV) groups, where I and II — non-stressed mice, III and IV — stressed animals, I and III received water with trace mineral solution (TMS), II and IV received water with SR. As stress factors, forced swimming, rodent predator odour or both together were applied. Locomotor activity under normal and stress conditions in Open Field were observed and measured by a SMART video-tracking system. Blood glucose level was measured as well. SR showed a reversal of stress-decreased locomotor activity in all stress models — distance walked increased almost twice (p < 0.0001), central zone crossings and time spent in it were 2–4 times greater than in the control group (p < 0.0001 and p = 0.0002, respectively), and fast movement episodes and maximal speed increased by 50–200%. In addition, complete normalisation of stress-induced elevation of blood glucose level (p < 0.0001) was noted. These results demonstrate for the first time that the effect of “Stress Relief” formula (water additives–minerals processed by know-how way in Vital Force Technology using Dr. Yury Kronn method) can be observed in laboratory animals, and that the effects are significant and repeatable. SR shows fear- and stress-reducing activity, which does not sufficiently differ between 7-, 9-, 14-, 28- and 32-day treatment regimes.


Andreescu, C., Mulsant, B. H., Emanuel, J. E. (2008). Complementary and alternative medicine in the treatment of bipolar disorder: A review of the evidence. J. Affect. Disord., 110, 16–26.10.1016/j.jad.2008.03.01518456339Search in Google Scholar

Anonymous (2013). The Structuring Effect of Vital Force Energetic Formulas in Water Solutions Verified by Gas Discharge Visualization (GDV) Camera. Available from: http://www.vitalforcetechnology.com/uploads/images/downloads/Measuring-Effects-with-GDV.pdf (accessed 4 August 2017).Search in Google Scholar

Anonymous (2017a). Bioseb Website. See: https://www.bioseb.com/bioseb/anglais/default/index.phpSearch in Google Scholar

Anonymous (2017b). MGI (Mouse Genome International) database. Mouse facts. Available from: http://www.informatics.jax.org/mgihome/other/mouse_facts1.shtml 2017 (accessed 4 August 2017).Search in Google Scholar

Anonymous (2017c). PennState Animal Resource Program. (2017). Porsolt Forced Swim Test. Available from: http://www.research.psu.edu/arp/experimental-guidelines/rodent-behavioral-tests-1/porsolt-forced-swim-test.html (accessed 4 August 2017).Search in Google Scholar

Anonymous (2017d). Quantum World TV. Kronn Y: Introduction to Energy Medicine. http://quantumworld.tv/videos/intro-to-energy-medicine/ (accessed 4 August 2017.Search in Google Scholar

Anonymous (2017e). Vital Force Technology website. Available from: http://www.vitalforcetechnology.com/research-library/advanced-research (accessed 4 August 2017).Search in Google Scholar

Anonymous (2017f). Waters website. http://www.waters.com/waters/en_LV/Original-UPLC-UHPLC-system-with-sub-2-micron-particletechnology-for-separations/nav.htm?cid=514207&locale=en_LV) (accessed 4 August 2017).Search in Google Scholar

Anonymous (2017g). World Summit of Integrative Medicine. Speaker Line-Up. Available from: https://worldsummitintegrativemedicine.com/speakers/ (accessed 4 August 2017).Search in Google Scholar

Axe, J. (2010). Conventional Medicine is the leading cause of death. Available from: http://draxe.com/conventional-medicine-is-the-leading-cause-of-death/ (accessed 4 August 2017).Search in Google Scholar

Bao, A.-M., Meynen, G., Swaab, D. F. (2008). The stress system in depression and neurodegeneration: Focus on the human hypothalamus. Brain Res. Rev., 57, 531–553.10.1016/j.brainresrev.2007.04.00517524488Search in Google Scholar

Berry, A., Bellisario, V., Capoccia, S., Tirassa, P., Calza, A., Alleva, E., Cirulli, F. (2012). Social deprivation stress is a triggering factor for the emergence of anxiety-and depression-like behaviours and leads to reduced brain BDNF levels in C57BL/6J mice. Psychoneuroendocrinology, 37, 762–772.10.1016/j.psyneuen.2011.09.00721974975Search in Google Scholar

Bet, P. M., Hugtenburg, J. G., Penninx, B. W. J. H., van Balkom, A., Nolen, W. A., Hoogendijk, W. J. G. (2013). Treatment inadequacy in primary and specialized care patients with depressive and/or anxiety disorders. Psychiatry Res., 210, 594–600.10.1016/j.psychres.2013.06.02323850429Search in Google Scholar

Bussey, T. J., Holmes, A., Lyon, L., Mar, A. C., McAllister, K. A., Nithianantharajah, J., Oomen, C. A., Saksida, L. M. (2012). New translational assays for preclinical modelling of cognition in schizophrenia: The touchscreen testing method for mice and rats. Neuropharmacology, 62, 1191–1203.10.1016/j.neuropharm.2011.04.011316871021530550Search in Google Scholar

Commons, K. G., Cholanians, A. B., Babb, J. A., Ehlinger, D. G. (2017). The rodent forced swim test measures stress-coping strategy, not depression-like behavior. ACS Chem. Neurosci., 8 (5), 955–960.10.1021/acschemneuro.7b00042551860028287253Search in Google Scholar

Cryan, J. F., Holmes, A. (2005). The ascent of mouse: Advances in modelling human depression and anxiety. Nat. Rev. Drug Discov., 4, 775–790.10.1038/nrd182516138108Search in Google Scholar

Forster, A. J., Murff, H. J., Peterson, J. F., Gandhi, T. K., Bates, D. W. (2003). The incidence and severity of adverse events affecting patients after discharge from the hospital. Ann. Intern. Med., 138 (3), 161–167.10.7326/0003-4819-138-3-200302040-0000712558354Search in Google Scholar

Gerbarg, P. L., Brown, R. P. (2001). Phytomedicines for prevention and treatment of mental health disorders. Psych. Clin. North Amer., 36, 37–47.10.1016/j.psc.2012.12.00423538075Search in Google Scholar

Gerber, R. (2001). Vibrational Medicine. Bear & Company. 608 pp.Search in Google Scholar

Gould, T. D., Dao, D. T.,, Kovacsics, C. E. (2009). The Open Field Test. In: Gould, T. D. (Ed.). Neuromethods V42, Mood and Anxiety Related Phenotypes in Mice. Humana Press. pp. 1–20.Search in Google Scholar

Han, J., Yang, M., Chen, Y. (2011). Quantum: May be a new-found messenger in biological systems. BioScience Trends, 5 (3), 89–92.10.5582/bst.2011.v5.3.8921788692Search in Google Scholar

Hefner, K., Holmes, A. (2007). Ontogeny of fear-, anxiety- and depression-related behaviour across adolescence in C57BL/6J mice. Behav. Brain Res., 176, 210–215.10.1016/j.bbr.2006.10.001183183817098297Search in Google Scholar

Heredia, L., Torrente, M., Colomina, M. T., Domingo, J. L. (2014). Assessing anxiety in C57BL/6J mice: A pharmacological characterization of the open-field and light/dark tests. J. Pharmacol. Toxicol. Meth., 9, 108–114.10.1016/j.vascn.2013.12.00524374104Search in Google Scholar

Jafari, Z., Kolb, B. E., Mohajerani, M. H. (2017). Effect of acute stress on auditory processing: A systematic review of human studies. Rev. Neurosci., 28 (1), 1–13.10.1515/revneuro-2016-004327718496Search in Google Scholar

Kemp, A. H., Gordon, E., Rush, A. J., Williams, L. M. (2008). Improving the prediction of treatment response in depression: Integration of clinical, cognitive, psychophysiological, neuroimaging, and genetic measures. CNS Spectrums, 13, 1066–1086.10.1017/S1092852900017120Search in Google Scholar

Kim, Y. N., Cho, S.-H. (2014). A survey of complementary and alternative medicine practitioner’s perceptions of evidence-based medicine. Eur. J. Integr. Med.. 6 (2), 211–219.10.1016/j.eujim.2013.11.001Search in Google Scholar

Kronn, Y., Eanes, R., Marongelle, J. (2001). Subtle energy and energy medicine: New paradigms, new technologies, new possibilities. Available from: http://vitalforcetechnology.com/uploads/images/downloads/Subtle-Energys-Interaction-with-the-Human-Body.pdf (accessed 4 August 2017).Search in Google Scholar

Kronn, Y. (2003). Energy—the missing link to health. New effective energy medicine tools. Science of whole person healing. In: Proceedings of the First Interdisciplinary International Conference, 28–30 March 2003, Bethesda, Maryland. Rustom Roy (Ed.), iUniverse, Inc., Lincoln, NE, pp. 157–171.Search in Google Scholar

Kronn, Y. (2009). Subtle Energy, the Universe and Us. The Science and Theory behind Vital Force Technology. Available from: http://www.energytoolsint.com/wp-content/uploads/2011/10/Subtle-Energy-The-Universe-and-Us.pdf (accessed 4 August 2017).Search in Google Scholar

Kronn, Y., Jones, J. (2011). Experiments on the Effects of Subtle Energy on the Electro-Magnetic Field. Is Subtle Energy the 5th Forcre of the Universe? Available from: http://www.goodhealthfrominsideout.com/pdf/emfdoc1.pdf (accessed 4 August 2017).Search in Google Scholar

Kunimoto, S., Nakamura, S., Wada, K., Inoue, T. (2010). Chronic stress-mutated presenilin 1 gene interaction perturbs neurogenesis and accelerates neurodegeneration. Exper. Neurol., 221, 175–185.10.1016/j.expneurol.2009.10.02019896484Search in Google Scholar

Lu, Z. (1997). Scientific Qigong Exploration. The Wonders and Mysteries of Qi. Amber Leaf Press, Malvern, PA. 404 pp.Search in Google Scholar

Luo, L. (2016). Chapter 6. Olfaction, Taste, Audition, and Somatosensation. In: Luo, L. (Ed.). Princ. Neurobiol. Taylor & Francis Group Publ., Garland Science, New York, pp. 207–275.Search in Google Scholar

Mao, Q.-Q., Xian, Y.-F., Ip, S.-P., Tsai, S.-H., Che, C.-T. (2010). Long-term treatment with peony glycosides reverses chronic unpredictable mild stress-induced depressive-like behaviour via increasing expression of neurotrophins in rat brain. Behav. Brain Res., 210, 171–177.10.1016/j.bbr.2010.02.02620176057Search in Google Scholar

Marzotto, M., Conforti, A., Magnani, P., Zanolin, M. E., Bellavite, P. (2012). Effects of Ignatia amara in mouse behavioural models. Homeopathy, 101, 57–67.10.1016/j.homp.2011.10.00122226316Search in Google Scholar

Mayor, D. F., Micozzi, M. S. (2011). Energy Medicine: East and West. Churchill Livingston. 420 pp.Search in Google Scholar

Null, G., Dean, C., Feldman, M., Rasio, D., Smith, D. (2006). Death by Medicine. Life Extension Magazine, August. Available from: http://www.lef.org/magazine/mag2006/aug2006_report_death_01.htm?source=search&key=death%20by%20medicine (accessed 4 August 2017).Search in Google Scholar

Oschman, J. L. (2016). Energy Medicine: The Scientific Basis. Elsevier Science. 359 pp.Search in Google Scholar

Qi, X., Lin, W., Li, J., Li, H., Wang, W., Wang, D., Sun, M. (2008). Fluoxetine increases the activity of the ERK-CREB signal system and alleviates the depressive-like behaviour in rats exposed to chronic forced swim stress. Neurobiol. Dis., 31, 278–285.10.1016/j.nbd.2008.05.00318586506Search in Google Scholar

Rao, M. N., Slawecki, T. M., Hoover, M. R., Roy, R. (2008). Characterization and properties of structured waters. “Materials Day” at the Materials Research Institute, Penn State University, 15 April. Available from: http://www.personal.psu.edu/~tms9/water.html (accessed 4 August 2017).Search in Google Scholar

Rindfleisch, J. A. (2010). Biofield Therapies: Energy Medicine and Primary Care. Primary Care: Clinics in Office Practice, 37, 165–179.10.1016/j.pop.2009.09.01220189005Search in Google Scholar

Ritchie, L., De Butte, M., Pappas, B. A. (2004). Chronic mild stress exacerbates the effects of permanent bilateral common carotid artery occlusion on CA1 neurons. Brain Res., 1014, 228–235.10.1016/j.brainres.2004.04.036Search in Google Scholar

Scholkmann, F., Fels, D., Cifra, M. (2013). Non-chemical and non-contact cell-to-cell communication: A short review. Amer. J. Transl. Res., 5 (6), 586–593.Search in Google Scholar

Smith, D. G., Davis, R. J., Gehlert, D. R., Nomikos, G. G. (2006). Exposure to predator odour stress increases efflux of frontal cortex acetylcholine and monoamines in mice: Comparisons with immobilization stress and reversal by chlordiazepoxide. Brain Res., 1117, 24–30.10.1016/j.brainres.2006.07.058Search in Google Scholar

Sonnentag, S., Fritz, C. (2006). Endocrinological processes associated with job stress: Catecholamine and cortisol responses to acute and chronic stressors. In: Perrewe, P. L., Ganster, D. C. (Eds.). Research in Occupational Stress and Well-being. Elsevier, pp. 1–59.10.1016/S1479-3555(05)05001-8Search in Google Scholar

Staples, L. G. (2010). Predator odour avoidance as a rodent model of anxiety: Learning-mediated consequences beyond the initial exposure. Neurobiol. Learning Memory, 94, 435–445.10.1016/j.nlm.2010.09.00920884366Search in Google Scholar

Swanson, C. (2009). Life Force, the Scientific Basis: Breakthrough Physics of Energy Medicine, Healing, Chi and Quantum Consciousness. Poseidia Press. 720 pp.Search in Google Scholar

van Reedt Dortland, A. K. B., Vreeburg, S. A., Giltay, E. J., Licht, C. M. M., Vogelzangs, N., van Veen, T., de Geus, E. J. C., Penninx, B. W. J. H., Zitman, F. G. (2013). The impact of stress systems and lifestyle on dyslipidemia and obesity in anxiety and depression. Psychoneuroendocrinology, 38, 209–218.10.1016/j.psyneuen.2012.05.01722717171Search in Google Scholar

Wahner-Roedler, D. L., Lee, M. C., Chon, T. Y., Cha, S. S., Loehrer, L. L., Bauer, B. A. (2014). Physicians’ attitudes toward complementary and alternative medicine and their knowledge of specific therapies: 8-Year follow-up at an academic medical center. Complem. Ther. Clin. Practice, 20, 54–60.10.1016/j.ctcp.2013.09.00324439646Search in Google Scholar

Wallace, D. (2012). Examining fear and stress as mediators between disorder perceptions and personal health, depression, and anxiety. Soc. Science Res., 41, 1515–1528.10.1016/j.ssresearch.2012.06.00523017971Search in Google Scholar

Wardle, J. L., Adams, J. (2014). Indirect and non-health risks associated with complementary and alternative medicine use: An integrative review. Eur. J. Integr. Med. 6 (4), 409–422.10.1016/j.eujim.2014.01.001Search in Google Scholar

Wilber, K. (2005). Toward a comprehensive theory of subtle energies. Explore, 1 (4), 252–270.10.1016/j.explore.2005.04.01616781546Search in Google Scholar

Young, J. W., Minassian, A., Paulus, M. P., Geyer, M. A. Perry, W. (2007). A reverse-translational approach to bipolar disorder: Rodent and human studies in the Behavioral Pattern Monitor. Neurosci. Biobehav. Rev., 31, 882–896.10.1016/j.neubiorev.2007.05.009202568817706782Search in Google Scholar

Recommended articles from Trend MD

Plan your remote conference with Sciendo