<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>β-glucanase is widely used in many fields and has great economic value and development space, but it faces the difficulties of separation and nutrient destruction in the process of industrial production. Foam separation is a simple, mild and efficient adsorption separation technique that enables efficient separation and extraction of β-glucanase. In this study, five single factors (loading volume, pH, separation gas velocity, fermentation loading concentration, surfactant concentration) of foam separation and harvest of β-glucanase produced by Pichia pastoris were studied. The best univariate condition was: 600 mL/min separation gas velocity, loading volume of 200 mL, initial enzyme concentration of 100 g/mL, surfactant concentration of 0.3 mg/mL and pH of 5. Based on the best univariate condition, the optimal separation conditions of β-glucanase were further explored, and the five-factor four-level orthogonal test was designed. From the experimental results, the best separation condition was: 600 mL/min, loading volume of 200 mL, initial enzyme concentration of 100 μg/mL, surfactant concentration of 0.5 mg/mL and pH of 5. Under this separation condition, the enrichment ratio (E) was 0.56 and the recovery rate (R) was 96.01%.</p>
</abstract>

β-glucanase is widely used in many fields and has great economic value and development space, but it faces the difficulties of separation and nutrient destruction in the process of industrial production. Foam separation is a simple, mild and efficient adsorption separation technique that enables efficient separation and extraction of β-glucanase. In this study, five single factors (loading volume, pH, separation gas velocity, fermentation loading concentration, surfactant concentration) of foam separation and harvest of β-glucanase produced by Pichia pastoris were studied. The best univariate condition was: 600 mL/min separation gas velocity, loading volume of 200 mL, initial enzyme concentration of 100 g/mL, surfactant concentration of 0.3 mg/mL and pH of 5. Based on the best univariate condition, the optimal separation conditions of β-glucanase were further explored, and the five-factor four-level orthogonal test was designed. From the experimental results, the best separation condition was: 600 mL/min, loading volume of 200 mL, initial enzyme concentration of 100 μg/mL, surfactant concentration of 0.5 mg/mL and pH of 5. Under this separation condition, the enrichment ratio (E) was 0.56 and the recovery rate (R) was 96.01%.

Application of foam separation in production of β-glucanase in Pichia

College of Chemistry and Chemical Engineering

Polish Journal of Chemical Technology

This work is licensed under the Creative Commons Attribution 4.0 International License.

{"article-title":"Application of foam separation in production of β-glucanase in Pichia"}

β-glucanase is widely used in many fields and has great economic value and development space, but it faces the difficulties of separation and nutrient...

Application of foam separation in production of β-glucanase in Pichia

Published Online: Oct 16, 2022

Page range: 1 - 7

DOI: https://doi.org/10.2478/pjct-2022-0015

Keywords
Foam separation, β-glucanase, recovery rate, enrichment ratio

© 2022 Qin Yuhang et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Application of foam separation in production of β-glucanase in Pichia

Published Online: Oct 16, 2022

Page range: 1 - 7

DOI: https://doi.org/10.2478/pjct-2022-0015

KeywordsFoam separation, β-glucanase, recovery rate, enrichment ratio

© 2022 Qin Yuhang et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
Foam separation, β-glucanase, recovery rate, enrichment ratio