Investigation of canine parvovirus occurrence in cats with clinical signs of feline panleukopenia in Slovakia – pilot study
, , , , , , , , and | Apr 15, 2024
About this article
Published Online: Apr 15, 2024
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Received: Oct 20, 2023
Accepted: Mar 28, 2024
DOI: https://doi.org/10.2478/jvetres-2024-0021
Keywords
© 2024 Alexandra Citarová et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Fig. 1.
Detailed clinical information on cats from central Slovakian animal shelters or veterinary clinics with signs of feline panleukopenia
| Group | Sample ID | Breed | Sex | Age | FPV vaccination | Anamnesis |
|---|---|---|---|---|---|---|
| I. clinical signs (n = 21) | 1 | crossbreed | female | 3 months | no | vomiting, diarrhoea |
| 2 | crossbreed | male | 3 months | no | vomiting, diarrhoea, lethargy | |
| 3 | crossbreed | female | 2 months | no | vomiting, diarrhoea, fever | |
| 4 | ESH | female | 6 months | no | lethargy, anorexia, fever | |
| 5 | ESH | female | 5 months | no | lethargy, anorexia | |
| 6 | crossbreed | female | 2 months | no | vomiting, diarrhoea | |
| 7 | crossbreed | male | 3 months | no | vomiting, lethargy | |
| 8 | crossbreed | female | 3 months | no | lethargy, fever | |
| 9 | crossbreed | female | 3 months | no | vomiting, diarrhoea, fever | |
| 10 | ESH | female | 3 years | no | lethargy, fever | |
| 11 | crossbreed | female | 4 months | yes | vomiting, lethargy, fever | |
| 12 | ESH | female | 3 years | no | vomiting, lethargy, anorexia | |
| 13 | crossbreed | male | 7 months | no | vomiting, lethargy, fever | |
| 14 | crossbreed | male | 6 months | no | diarrhoea, fever, vomiting | |
| 15 | crossbreed | male | 5 years | yes | diarrhoea | |
| 16 | ESH | male | 3 months | no | vomiting, lethargy, fever | |
| 17 | ESH | male | 5 months | no | vomiting, lethargy, fever, diarrhoea | |
| 18 | crossbreed | male | 5 months | no | vomiting, lethargy, fever | |
| 19 | ESH | male | 6 months | no | vomiting, lethargy, fever | |
| 20 | crossbreed | male | 1 year | no | vomiting, diarrhoea | |
| 21 | crossbreed | male | 3 months | no | diarrhoea, vomiting | |
| II. close contact with parvovirus-infected animal (n = 29) | 1 | crossbreed | female | 4 years | yes | healthy |
| 2 | crossbreed | female | 6 months | no | healthy | |
| 3 | crossbreed | male | 4 months | no | healthy | |
| 4 | crossbreed | female | 4 years | yes | healthy | |
| 5 | crossbreed | female | 5 years | yes | healthy | |
| 6 | ESH | female | 2 years | no | healthy | |
| 7 | ESH | female | 1 year | no | healthy | |
| 8 | ESH | female | 4 years | yes | healthy | |
| 9 | crossbreed | male | 5 years | yes | healthy | |
| 10 | ESH | female | 4 months | no | diarrhoea | |
| 11 | crossbreed | female | 3 months | no | diarrhoea | |
| 12 | crossbreed | male | 2 months | no | diarrhoea | |
| 13 | crossbreed | female | 3 months | no | diarrhoea, vomiting | |
| 14 | crossbreed | male | 3 months | no | healthy | |
| 15 | ESH | female | 1 year | no | healthy | |
| 16 | crossbreed | male | 5 months | no | healthy | |
| 17 | crossbreed | male | 2 years | yes | healthy | |
| 18 | crossbreed | male | 2 years | yes | intermittent diarrhoea | |
| 19 | crossbreed | female | 5 months | no | diarrhoea, overcame FPL | |
| 20 | ESH | female | 5 years | no | healthy | |
| 21 | crossbreed | male | 2 years | yes | intermittent diarrhoea | |
| 22 | crossbreed | female | 4 months | no | healthy | |
| 23 | ESH | female | 2 years | no | healthy | |
| 24 | crossbreed | female | 1 year | no | healthy, overcame FPL | |
| 25 | crossbreed | male | 1 year | yes | healthy | |
| 26 | crossbreed | female | 5 months | no | diarrhoea, overcame FPL | |
| 27 | crossbreed | male | 4 months | no | vomiting | |
| 28 | crossbreed | female | 3 years | yes | healthy | |
| 29 | crossbreed | female | 6 months | no | healthy, overcame FPL | |
| III. clinically healthy (n = 9) | 1 | ESH | male | 1 year | yes | healthy |
| 2 | ESH | female | 2 years | yes | healthy | |
| 3 | crossbreed | male | 7 months | yes | healthy | |
| 4 | crossbreed | female | 3 years | yes | healthy | |
| 5 | crossbreed | male | 3 months | yes | healthy | |
| 6 | crossbreed | male | 5 months | yes | healthy | |
| 7 | crossbreed | female | 1 year | yes | healthy | |
| 8 | crossbreed | female | 2 years | yes | healthy | |
| 9 | ESH | male | 1 year | yes | healthy |
Antigen-based detection of parvovirus infection in rectal swab samples of European shorthair and crossbred cats in central Slovakia grouped by feline panleukopenia status
| Group | Sample ID | CPV antigen test |
|---|---|---|
| I. clinical signs (n = 21) | 1–21 | + |
| II. close contact with parvovirus-infected animal (n = 29) | 1–9; 11–26; 28, 29 | - |
| 10, 27 | + | |
| III. clinically healthy (n = 9) | 1–9 | - |
Summary of parvovirus infection findings in rectal swab samples of European shorthair and crossbred cats in central Slovakia grouped by feline panleukopenia status
| Groups | CPV antigen test | PCR test | Total positive | ||
|---|---|---|---|---|---|
| Ag+ | Ag- | PCR+ | PCR- | ||
| I. clinical signs (n = 21) | 21 | 0 | 21 | 0 | 21 (100%) |
| II. close contact with parvovirus-infected animals (n = 29) | 2 | 27 | 2 | 27 | 2 (6.89%) |
| III. clinically healthy (n = 9) | 0 | 9 | 0 | 9 | 0 |
Sequences producing significant alignments with the VP2 gene amplicons of investigated rectal swab samples from European shorthair and crossbred cats in central Slovakia. Sequence analysis used the National Center for Biotechnology Information BLASTn algorithm
| Scientific name | Pairwise identity (%) | Query coverage (%) | GenBank accession number |
|---|---|---|---|
| Feline panleukopenia virus | 100.00 | 100 | MK671185.1, MK671183.1, MK671171.1, MK671170.1, MK671156.1, MK425504.1, MK425500.1, MK425499.1, MK425498.1, MK425497.1, KP019621.2, MG764511.1, MG764510.1, MF541133.1, MF541132.1, MF541119.1, KX685354.1, KX900570.1, KT240130.1, KT357491.1, OP796716.1, OP796714.1, KP682520.1, KP019617.1, OM638042.1, MW091486.1, MW091487.1, MZ712026.1, MZ322607.1, MZ357122.1, MZ357120.1, MZ357119.1, MW017627.1, MT270583.1, MW331496.1, MN419003.1, MT274378.1, HQ184201.1, FJ936171.1, DQ474238.1, DQ474237.1, DQ474236.1, EU221281.1, AY606131.1, DQ099430.1, AY955826.1, DQ003301.1, AB054227.1, AB054226.1, AB054225.1 |
| Feline parvovirus | 100.00 | 100 | MK266790.1, MK266788.1, MK295775.1, OM885375.1, ON646210.1, MZ442303.1, OR211675.1 |
| Feline panleukopenia virus | 99.78 | 100 | OQ863619.1, OQ863618.1, OQ863617.1, OQ863615.1, MZ508524.1 |
| Feline parvovirus | 99.78 | 100 | OR198066.1, OQ869254.1, OQ868569.1, OQ868568.1, OQ868566.1, OQ868565.1, OQ868564.1, OQ868562.1, OQ868554.1, OQ868553.1, OQ868552.1, OQ868550.1, OQ868548.1, OQ868547.1, OQ868542.1, OQ868536.1, OQ868535.1, OQ868534.1, OR194134.1, OR194132.1, OR194130.1, OR194129.1, OR194128.1, OR194125.1, OR194122.1, OQ535514.1, OQ535513.1, OQ535511.1, OQ535510.1, OQ535509.1 |