<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec id="j_jvetres-2023-0005_s_005"><h3>Introduction</h3>
<p>Canine periodontitis results among other factors from a disturbed balance of dental plaque microflora and an inadequate host inflammatory response to a stimulus. This investigation sought to identify microorganisms associated with canine periodontitis.</p>
</sec>
<sec id="j_jvetres-2023-0005_s_006"><h3>Material and Methods</h3>
<p>Microbiological analysis was undertaken of gingival pockets in an experimental group of 36 dogs with periodontal diseases. Swabs were collected with the use of Pet Test (MIP Pharma, Berlin, Germany) from patients with gingival pockets deeper than 5 mm. Samples were aggregated and placed in separate shipping containers with the Pet Test kit.</p>
</sec>
<sec id="j_jvetres-2023-0005_s_007"><h3>Results</h3>
<p>Identification was made of the most common microorganisms, <italic>e.g</italic>. <italic>Porphyromonas gingivalis</italic>, <italic>Treponema denticola</italic> and <italic>Prevotella intermedia</italic>. The red complex constituted the largest proportion of all analysed organisms (84.26%). <italic>Capnocytophaga gingivalis</italic> was isolated from 33 dogs, <italic>Peptostreptococcus micros</italic> from 32 dogs, <italic>Fusobacterium nucleatum</italic> from 29 animals and <italic>P. intermedia</italic> from 20.</p>
</sec>
<sec id="j_jvetres-2023-0005_s_008"><h3>Conclusion</h3>
<p>The highest percentage of pathogens was supplied by <italic>P. gingivalis</italic> (61%). It is thought that dogs acquire them by means of cross-species transmission. The inter-study variability of results may depend not only on the method of periopathogen detection, but also on environmental factors, host immune status or genetic background. Depending on the state of periodontal disease, patients show varied microbiological profiles of the gingival pockets.</p>
</sec>
</abstract>

Introduction
Canine periodontitis results among other factors from a disturbed balance of dental plaque microflora and an inadequate host inflammatory response to a stimulus. This investigation sought to identify microorganisms associated with canine periodontitis.

Material and Methods
Microbiological analysis was undertaken of gingival pockets in an experimental group of 36 dogs with periodontal diseases. Swabs were collected with the use of Pet Test (MIP Pharma, Berlin, Germany) from patients with gingival pockets deeper than 5 mm. Samples were aggregated and placed in separate shipping containers with the Pet Test kit.

Results
Identification was made of the most common microorganisms, e.g. Porphyromonas gingivalis, Treponema denticola and Prevotella intermedia. The red complex constituted the largest proportion of all analysed organisms (84.26%). Capnocytophaga gingivalis was isolated from 33 dogs, Peptostreptococcus micros from 32 dogs, Fusobacterium nucleatum from 29 animals and P. intermedia from 20.

Conclusion
The highest percentage of pathogens was supplied by P. gingivalis (61%). It is thought that dogs acquire them by means of cross-species transmission. The inter-study variability of results may depend not only on the method of periopathogen detection, but also on environmental factors, host immune status or genetic background. Depending on the state of periodontal disease, patients show varied microbiological profiles of the gingival pockets.

Molecular microbiological characteristics of gingival pockets in the periodontal diseases of dogs

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction
Canine periodontitis results among other factors from a disturbed balance of dental plaque microflora and an inadequate host inflammatory response...

Subgroups		Aa	Pg	Td	Tf	Pi	Pm	Fn	En	Cg
	x	-	41.00	13.06	-	1.18	1.10	4.62	0.01	0.30
E2
	SD	-	37.50	8.30	-	0.32	0.71	3.61	-	0.18

	x	-	47.56	26.72	0.15	5.35	1.32	10.13	0.18*	0.43
E3
	SD	-	38.61	39.70	0.11	7.91	2.63	14.69	0.22	0.42

	x	-	89.35	27.42	0.25	4.15	4.66	9.69	0.10*	1.02
E4
	SD	-	136.57	33.58	0.41	11.16	12.39	17.14	0.17	1.39

Molecular microbiological characteristics of gingival pockets in the periodontal diseases of dogs

Published Online: Feb 07, 2023

Page range: 115 - 122

Received: Jun 09, 2022

Accepted: Jan 27, 2023

DOI: https://doi.org/10.2478/jvetres-2023-0005

Keywords
periodontal diseases, gingival diseases, dogs, oral diseases, infectious diseases

© 2023 I. Polkowska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Fig. 2

Fig. 3

Mean number of bacteria isolated from gingival pockets presented in three subgroups

Molecular microbiological characteristics of gingival pockets in the periodontal diseases of dogs

Published Online: Feb 07, 2023

Page range: 115 - 122

Received: Jun 09, 2022

Accepted: Jan 27, 2023

DOI: https://doi.org/10.2478/jvetres-2023-0005

Keywordsperiodontal diseases, gingival diseases, dogs, oral diseases, infectious diseases

© 2023 I. Polkowska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Fig. 2

Fig. 3

Mean number of bacteria isolated from gingival pockets presented in three subgroups

Keywords
periodontal diseases, gingival diseases, dogs, oral diseases, infectious diseases