High prevalence of Plasmodium infection in fighting cocks in Thailand determined with a molecular method
Published Online: Sep 15, 2022
Page range: 373 - 379
Received: Apr 22, 2022
Accepted: Sep 02, 2022
DOI: https://doi.org/10.2478/jvetres-2022-0049
Keywords
© 2022 K. Vaisusuk et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Introduction
Avian malaria caused by
Material and Methods
This study used microscopic examination of blood samples and PCR amplification exploiting primers for nucleotide sequences of
Results
Examination of 249 blood samples of fighting cocks revealed that 41.37% (103/249) were positive for malaria by microscopic examination and 88.76% (221/249) were positive by DNA amplification. Sequencing and DNA analysis of 61 PCR products revealed that infection by
Conclusion
This study indicated that plasmodiasis is widespread in fighting cocks in Thailand although the prevalence was not clearly determined; therefore, prevention and control strategies for these protozoa should be improved, especially those for avoiding vector exposure and eliminating mosquito breeding sites.