Ex vitro Rooting, Acclimatization and Genetic Stability of Lonicera caerulea var. kamtschatica
, and | Dec 31, 2020
About this article
Published Online: Dec 31, 2020
Page range: 61 - 70
Received: May 01, 2020
Accepted: Sep 01, 2020
DOI: https://doi.org/10.2478/johr-2020-0019
Keywords
© 2020 Agnieszka Wojtania et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Figure 1
The effect of microcutting type and soil substrate type on ex vitro rooting and acclimatization of Lonicera caerulea ‘Zojka’ and ‘Wojtek’ in the greenhouse
| Cutting type | Soil substrate | Rooted/survived plants (%) | Fresh mass of shoots (mg) | Shoot length (mm) | Fresh root mass (mg) | Root number/cutting | Root length (mm) |
|---|---|---|---|---|---|---|---|
| ‘Zojka’ | |||||||
| Individual shoots | peat | 88 a | 91.9 ± 20.0c | 81.4 ± 7.0a | 22.0 ± 8.7 c | 2.8 ± 1.1a | 25.6 ± 5.6a |
| peat + sand | 80 a | 52.9 ± 9.0d | 62.3 ± 8.0c | 11.0 ± 3.6d | 2.7 ± 0.7a | 24.4 ± 5.8a | |
| peat + perlite | 84 a | 62.0 ± 17.0cd | 69.0 ± 14.0bc | 15.4 ± 8.3cd | 2.5 ± 0.7a | 23.7 ± 7.1a | |
| Multiplied clumps | peat | 68 a | 127.7 ± 41.0b | 68.4 ± 14.0bc | 22.8 ± 8.7 c | 1.8 ± 0.5b | 12.0 ± 1.9b |
| peat + sand | 72 a | 139.3 ± 75.0ab | 50.3 ± 12.0d | 33.0 ± 21.3b | 2.9 ± 1.5a | 15.3 ± 6.5b | |
| peat + perlite | 84 a | 164.2 ± 88.0a | 73.6 ± 22.9ab | 49.6 ± 24.4a | 3.0 ± 0.7a | 21.8 ± 7.5a | |
| ‘Wojtek’ | |||||||
| Individual shoots | peat | 96 a | 97.8 ± 20.0bc | 106.8 ± 14.9a | 20.9 ± 6.6c | 2.4 ± 0.7b | 27.1 ± 7.4a |
| peat + sand | 80 ab | 72.4 ± 20.0c | 85.8 ± 15.0b | 12.9 ± 6.9c | 1.9 ± 0.4c | 29.4 ± 8.1a | |
| peat + perlite | 88 a | 114.2 ± 54.0bc | 112.3 ± 29.0a | 24.0 ± 9.5bc | 1.9 ± 0.5c | 21.8 ± 4.5b | |
| Multiplied clumps | peat | 60 b | 133.9 ± 34.0b | 66.6 ± 14.0cd | 36.3 ± 19.6b | 2.0 ± 0.5bc | 22.0 ± 6.8b |
| peat + sand | 76 ab | 103.4 ± 18.0bc | 60.4 ± 11.0d | 20.0 ± 7.1c | 1.6 ± 0.7c | 10.8 ± 2.9c | |
| peat + perlite | 88 a | 191.2 ± 43.0a | 73.8 ± 30.0bc | 71.8 ± 33.0a | 3.1 ± 1.0a | 28.4 ± 12.1a | |
The genetic evaluation of acclimatized plantlets of Lonicera caerulea ‘Wojtek’ and ‘Zojka’ compared to mother plants
| No. | Primer | Total number of bands | Number of polymorphic bands | Range of amplicon (pb) | Total number of bands | Number of polymorphic bands | Range of amplicon (pb) |
|---|---|---|---|---|---|---|---|
| ‘Zojka’ | ‘Wojtek’ | ||||||
| 1 | UBC 825 | 9 | 1 | 500–1000 | 11 | 0 | 400–1000 |
| 2 | UBC 810 | 6 | 0 | 600–1300 | 5 | 0 | 500–1300 |
| 3 | UBC 827 | 12 | 0 | 300–800 | 11 | 0 | 300–750 |
| 4 | UBC 865 | 9 | 0 | 300–1400 | 13 | 0 | 300–1400 |
| Total | 36 | 1 | 40 | 0 | |||
| Proportion of polymorphic bands (%) | 2,77 | 0,00 | |||||
| 1 | Pst-CC/Mse-CC | 19 | 1 | 80–300 | 16 | 1 | 50–300 |
| 2 | Pst-TT/Mse-CC | 35 | 1 | 250–900 | 30 | 0 | 250–900 |
| 3 | Pst-GC/Mse-TA | 18 | 0 | 300–850 | 16 | 0 | 400–850 |
| 4 | Pst-CG/Mse-AG | 42 | 0 | 200–760 | 40 | 0 | 200–750 |
| Total | 105 | 2 | 102 | 1 | |||
| Proportion of polymorphic bands (%) | 1,90 | 0,98 | |||||
Primers, polymorphism between tested cultivars and amplification products of ISSR-PCR and AFLPPCR used for evaluation of genetic identity of Lonicera caerulea ‘Wojtek’ and ‘Zojka’ plants
| No. | Primer | Sequence (5′–3′) | Total number of bands | Polymorphic bands (%) | Primer | Sequence (5′–3′) | Total number of bands | Polymorphic bands (%) |
|---|---|---|---|---|---|---|---|---|
| ISSR | AFLP | |||||||
| 1 | UBC 806 | (TA)8G | - | - | Pst-TT/Mse-CC | GACTGCGTACATGCAGTT | 30 | 13.3 |
| 2 | UBC 810 | (GA)8T | 6 | 16.7 | Pst-GC/Mse-GC | GACTGCGTACATGCAGGC | 21 | 0 |
| 3 | UBC 813 | (CT)8T | - | - | Pst-GC/Mse-TA | GACTGCGTACATGCAGGC | 18 | 11.1 |
| 4 | UBC 821 | (GT)8T | - | - | Pst-TA/Mse-TA | GACTGCGTACATGCAGTA | 14 | 0 |
| 5 | UBC 822 | (TC)8A | 9 | 0 | Pst-TA/Mse-GC | GACTGCGTACATGCAGTA | 42 | 0 |
| 6 | UBC 823 | (TC)8C | 5 | 0 | Pst-AG/Mse-AG | GACTGCGTACATGCAGAG | 54 | 0 |
| 7 | UBC 825 | (AC)8T | 7 | 28.6 | Pst-AG/Mse-CG | GACTGCGTACATGCAGAG | 36 | 0 |
| 8 | UBC 827 | (AC)8G | 12 | 8.3 | Pst-CG/Mse-AG | GACTGCGTACATGCAGCG | 42 | 4.8 |
| 9 | UBC 828 | (TG)8A | 2 | 0 | Pst-TC/Mse-TC | GACTGCGTACATGCAGTC | 13 | 0 |
| 10 | UBC 830 | (TG)8G | 1 | 0 | Pst-TC/Mse-AT | GACTGCGTACATGCAGTC | 20 | 0 |
| 11 | UBC 834 | (AG)8CT | 3 | 0 | Pst-AT/Mse-AT | GACTGCGTACATGCAGAT | 50 | 0 |
| 12 | UBC 840 | (GA)8CT | 2 | 0 | Pst-AT/Mse-TC | GACTGCGTACATGCAGAT | 25 | 0 |
| 13 | UBC 843 | (CT)8GA | 1 | 0 | Pst-AA/Mse-AA | GACTGCGTACATGCAGAA | 13 | 0 |
| 14 | UBC 846 | (CA)8GT | 3 | 0 | Pst-AA/Mse-AC | GACTGCGTACATGCAGAA | 37 | 0 |
| 15 | UBC 853 | (TC)8AT | 6 | 0 | Pst-AC/Mse-AC | GACTGCGTACATGCAGAC | 12 | 0 |
| 16 | UBC 848 | (CA)8GG | - | - | Pst-AC/Mse-AA | GACTGCGTACATGCAGAC | 8 | 0 |
| 17 | UBC 849 | (GT)8CA | - | - | Pst-GG/Mse-GG | GACTGCGTACATGCAGGG | - | - |
| 18 | UBC 855 | (AC)8CT | 4 | 0 | Pst-GG/Mse-GA | GACTGCGTACATGCAGGG | - | - |
| 19 | UBC 858 | (TG)8AT | - | - | Pst-GA/Mse-GA | GACTGCGTACATGCAGGA | 19 | 0 |
| 20 | UBC 865 | (CCG)6 | 4 | 25.0 | Pst-GA/Mse-GC | GACTGCGTACATGCAGGA | 9 | 0 |
| 21 | UBC 867 | (GGC)6 | - | - | Pst-CC/Mse-CC | GACTGCGTACATGCAGCC | 20 | 30.0 |
| 22 | UBC 875 | (CTAG)4 | - | - | Pst-CC/Mse-GG | GACTGCGTACATGCAGCC | 43 | 0 |
| 23 | UBC 881 | (GGGT)3G | - | - | Pst-TT/Mse-CC | GACTGCGTACATGCAGTT | 48 | 0 |
The post-effect of auxin type (IAA and IBA) and concentration (0.0; 1.0; 2.5; 4.0 mg·dm−3) on ex vitro acclimatization of Lonicera caerulea ‘Zojka’ and ‘Wojtek’ in the growth chamber
| Genotype | Auxin type/concentration (mg·dm−3) | Survived micro-cutting (%) | Fresh mass of shoots (mg) | Shoot length (mm) | Fresh root mass (mg) | Root number/cutting | Root length (mm) | |
|---|---|---|---|---|---|---|---|---|
| ‘Zojka’ | IBA | 0.0 | 84 a | 184 ± 46.9bc | 115 ± 11.0bc | 52.6 ± 20.7c | 2.5 ± 0.9c | 38.5 ± 9.8ab |
| 1.0 | 88 a | 247 ± 69.7a | 154 ± 21.7a | 72.2 ± 35.0b | 3.7 ± 1.0ab | 37.1 ± 10.7ab | ||
| 2.5 | 80 a | 174 ± 68.8bc | 126 ± 11.3b | 63.6 ± 23.4bc | 3.8 ± 0.7a | 32.4 ± 15.8bc | ||
| 4.0 | 76 a | 179 ± 63.5bc | 116 ± 17.4bc | 54.8 ± 11.9bc | 3.7 ± 1.0ab | 28.7 ± 7.3c | ||
| IAA | 1.0 | 80 a | 219 ± 77.4ab | 111 ± 16.3c | 89.0 ± 36.7a | 3.5 ± 1.1ab | 42.0 ± 14.0a | |
| 2.5 | 76 a | 160 ± 67.9c | 117 ± 15.0bc | 48.4 ± 14.9 c | 3.3 ± 0.9ab | 36.2 ± 11.4a-c | ||
| 4.0 | 72 a | 142 ± 67.1c | 114 ± 22.6bc | 52.6 ± 28.7c | 3.1 ± 0.9bc | 33.1 ± 7.1bc | ||
| ‘Wojtek’ | IBA | 0.0 | 80 a | 218 ± 85.1b | 126 ± 32.4b | 42.7 ± 20.4 d | 2.5 ± 0.7d | 51.3 ± 8.5b |
| 1.0 | 88 a | 349 ± 95.4a | 181 ± 23.1a | 120.1 ± 43.5a | 3.7 ± 1.1ab | 44.3 ± 7.8bc | ||
| 2.5 | 84 a | 198 ± 73.8b | 135 ± 29.7b | 75.6 ± 22.4bc | 3.6 ± 0.9ab | 37.1 ± 8.8cd | ||
| 4.0 | 68 a | 160 ± 81.5b | 124 ± 38.5b | 74.8 ± 25.7bc | 3.2 ± 0.7 b-d | 33.3 ± 8.5d | ||
| IAA | 1.0 | 84 a | 339 ± 50.3a | 146 ± 32.4b | 86.3 ± 34.7b | 4.1 ± 1.2a | 59.0 ± 18.9a | |
| 2.5 | 76 a | 210 ± 61.1b | 145 ± 17.2b | 70.8 ± 26.4bc | 3.4 ± 1.1bc | 30.5 ± 6.8d | ||
| 4.0 | 76 a | 216 ± 58.4b | 140 ± 39.0b | 58.5 ± 21.8cd | 2.9 ± 1.0cd | 42.4 ± 14.7c | ||