Efficiency of Gaseous Ozone in Disinfection of Mushroom Growing Rooms

Joanna Szumigaj-Tarnowska; Piotr Szafranek; Zbigniew Uliński; Czesław Ślusarski

Open Access

Efficiency of Gaseous Ozone in Disinfection of Mushroom Growing Rooms

Joanna Szumigaj-Tarnowska

Piotr Szafranek

Zbigniew Uliński

and

Czesław Ślusarski

| Dec 31, 2020

Journal of Horticultural Research

Volume 28 (2020): Issue 2 (December 2020)

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Page range: 91 - 100

Received: Mar 01, 2020

Accepted: Sep 01, 2020

DOI: https://doi.org/10.2478/johr-2020-0017

Keywords
fungal pathogens, ozonation, control

© 2020 Joanna Szumigaj-Tarnowska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The gas concentration during the ozonation process at different exposure times

Effect of ozone exposure duration on the percentage of the colony growth inhibition of tested pathogens

Percentage of the growth inhibition of pathogens after different exposure times to ozonation

Sensitivity of pathogenic species to ozonation based on the average rate of colonization of the plate area (%) after 5 days of incubation in relation to ozone exposure time

Species	Treatment	Time of exposure

		8 h	6 h	4 h 2 h		1 h	0.5 h
Trichoderma aggressivum	Control	86.2 ± 7.3 a	78.8 ± 6.9 a	74.6 ± 5.2 a	76.7 ± 8.0 a	77.1 ± 7.6 a	76.7 ± 8.3 a
Trichoderma aggressivum	Ozone	31.7 ± 8.4 b	63.1 ± 6.5 a	59.2 ± 9.7 a	73.7 ± 5.8 a	75.0 ± 4.9 a	73.3 ± 4.4 a

Mycogone perniciosa	Control	55.0 ± 5.6 a	52.5 ± 5.9 a	65.0 ± 4.4 a	55.8 ± 8.0 a	55.4 ± 2.8 a	54.6 ± 6.2 a
Mycogone perniciosa	Ozone	0.0 ± 0.0 b	0.0 ± 0.0 b	44.5 ± 4.5 a	42.5 ± 6.3 a	52.9 ± 4.0 a	52.5 ± 5.5 a

Cladobotryum dendroides	Control	70.4 ± 3.4 a	60.0 ± 7.9 a	67.9 ± 5.3 a	72.1 ± 6.9 a	70.8 ± 3.9 a	67.1 ± 3.6 a
Cladobotryum dendroides	Ozone	0.0 ± 0.0 b	22.1 ± 1.4 b	63.7 ± 4.5 a	70.4 ± 5.5 a	69.2 ± 4.3 a	63.3 ± 5.0 a

Lecanicillium fungicola	Control	23.4 ± 3.1 a	23.1 ± 2.6 a	24.7 ± 3.3 a	26.2 ± 2.1 a	25.3 ± 2.8 a	25.8 ± 3.7 a
Lecanicillium fungicola	Ozone	0.0 ± 0.0 b	0.0 ± 0.0 b	0.0 ± 0.0	0.0 ± 0.0 b	0.0 ± 0.0	0.0 ± 0.0

Sensitivity of Trichoderma aggressivum isolates to ozonation, based on the percentage of growth inhibition

Days	Isolate	Time of exposure						Mean

		8 h	6 h	4 h	2 h	1 h	0.5 h
2	Th.12	100.0 ± 0.0 Aa	58.22 ± 15.6 Aab	49.56 ± 15.9 Bbc	38.1 ± 5.2 ABbc	30.30 ± 11.5A Abc	0.0 ± 0.0 Ac	46.03 A

	Th 3.9	100.0 ± 0.0 Aa	100.0 ± 0.0 Aa	100.0 ± 0.0 Aa	74.75 ± 2.2 Aa	18.82 ± 8.9 Ab	0.0 ± 0.0 Ab	65.60 A

	Th. 22.07	30.60 ± 10.9 Ba	11.25 ± 8.5 Ba	7.67 ± 4.2 Ba	15.8 ± 6.3 Ba	5.10 ± 3.3 Aa	0.0 ± 0.0 Ac	11.74 B

3	Th.12	89.89 ± 5.8 Aa	14.67 ± 5.3 Bb	10.12 ± 2.7 Bb	12.54 ± 2.6 Ab	12.30 ± 4.1 Ab	5.75 ± 3.3 Ab	24.21 AB

	Th 3.9	100.0 ± 0.0 Aa	100.0 ± 0.0 Aa	88.25 ± 1.1 Ab	17.15 ± 5.2 Ab	5.60 ± 3.1 Ab	0.0 ± 0.0 Ab	51.83 A

	Th. 22.07	32.82 ± 11.4 Ba	11.57 ± 2.5 Ba	15.49 ± 5.7 Ba	10.92 ± 2.9 Aa	17.50 ± 3.7 Aa	7.31 ± 4.4 Aa	15.93 B

4	Th.12	66.95 ± 7.1 ABa	21.44 ± 9.7 Bab	12.70 ± 2.2 Ab	8.01 ± 2.5 Ab	0.0 ± 0.0 Ab	0.0 ± 0.0 Ab	18.18 AB

	Th 3.9	100.0 ± 0.0 Aa	100.0 ± 0.0 Aa	49.94 ± 8.9 Ab	3.50 ± 2.1 Ac	2.55 ± 1.8 Ac	0.0 ± 0.0 Ac	42.67 A

	Th. 22.07	26.52 ± 14.8 Ba	6.97 ± 2.4 Ba	0.0 ± 0.0 Aa	0.0 ± 0.0 Aa	0.0 ± 0.0 Aa	0.0 ± 0.0 Aa	5.58 B

5	Th.12	16.81 ± 7.9 Ba	5.9 ± 1.6 Ba	0.0 ± 0.0 Aa	0.0 ± 0.0 Aa	0.0 ± 0.0 Aa	0.0 ± 0.0 Aa	3.78 B

	Th 3.9	100.0 ± 0.0 Aa	100.0 ± 0.0 Aa	27.50 ± 2.5 Ab	0.0 ± 0.0 Ab	0.0 ± 0.0 Ab	0.0 ± 0.0 Ab	45.92 A

	Th. 22.07	0.0 ± 0.0 Aa	0.0 ± 0.0 Aa	0.0 ± 0.0 Aa	0.0 ± 0.0 Aa	0.0 ± 0.0 Aa	0.0 ± 0.0 Aa	0.00 B

List of isolates used in this study and their origin

Species	Isolate code	Province	Year of collection
Trichoderma aggressivum	Th 3.9	Mazowieckie	2008
	Th.12	Mazowieckie	2009
	Th 22.07	Łódzkie	2005
Mycogone perniciosa	M9	Mazowieckie	2008
	M10	Podkarpackie	2008
	M18	Łódzkie	2009
Cladobotryum dendroides	D12A	Wielkopolskie	2008
	D16	Wielkopolskie	2008
	D30	Mazowieckie	2008
Lecanicillium fungicola	V-3	Mazowieckie	2005
	V-17	Mazowieckie	2009
	V-31	Łódzkie	2010

Sensitivity of Cladobotryum dendroides isolates to ozonation, based on the percentage of growth inhibition

Days	Isolate	Time of exposure						Mean

		8 h	6 h	4 h	2 h	1 h	0.5 h
2	D12A	100.0 ± 0.0 Aa	100.0 ± 0.0 Aa	0.0 ± 0.0 Ab	0.0 ± 0.0 Ab	0.0 ± 0.0 Ab	0.0 ± 0.0 Ab	33.33 A

	D16	100.0 ± 0.0 Aa	100.0 ± 0.0 Aa	0.0 ± 0.0 Ab	0.0 ± 0.0 Ab	0.0 ± 0.0 Ab	0.0 ± 0.0 Ab	33.33 A

	D30	100.0 ± 0.0 Aa	46.30 ± 15.5 Aab	29.7 ± 5.2 Ab	0.0 ± 0.0 Ab	0.0 ± 0.0 Ab	0.0 ± 0.0 Ab	29.33 A

3	D12A	100.0 ± 0.0 Aa	100.0 ± 0.0 Aa	20.67 ± 3.5 Ab	0.0 ± 0.0 Ab	0.0 ± 0.0 Ab	0.0 ± 0.0 Ab	36.78 A

	D16	100.0 ± 0.0 Aa	100.0 ± 0.0 Aa	13.29 ± 2.0 Ab	0.0 ± 0.0 Ab	0.0 ± 0.0 Ab	0.0 ± 0.0 Ab	35.55 A

	D30	100.0 ± 0.0 Aa	31.88 ± 9.2 Aab	20.87 ± 8.2 Ab	5.50 ± 2.5 Ab	0.0 ± 0.0 Ab	0.0 ± 0.0 Ab	26.38 A

4	D12A	100.0 ± 0.0 Aa	100.0 ± 0.0 Aa	10.75 ± 5.5 Ab	0.0 ± 0.0 Ab	0.0 ± 0.0 Ab	0.0 ± 0.0 Ab	35.12 A

	D16	100.0 ± 0.0 Aa	100.0 ± 0.0 Aa	18.39 ± 7.1 Ab	11.38 ± 4.5 Ab	0.0 ± 0.0 Ab	0.0 ± 0.0 Ab	38.30 A

	D30	100.0 ± 0.0 Aa	12.92 ± 5.0 Ab	13.20 ± 6.6 Ab	0.0 ± 0.0 Ab	0.0 ± 0.0 Ab	0.0 ± 0.0 Ab	21.01 A

5	D12A	100.0 ± 0.0 Aa	100.0 ± 0.0 Aa	9.68 ± 1.7 Aa	0.0 ± 0.0 Aa	0.0 ± 0.0 Aa	0.0 ± 0.0 Aa	34.95 A

	D16	100.0 ± 0.0 Aa	100.0 ± 0.0 Aa	27.5 ± 2.5 Aa	0.0 ± 0.0 Aa	0.0 ± 0.0 Aa	0.0 ± 0.0 Aa	37.92 A

	D30	100.0 ± 0.0 Aa	0.0 ± 0.0 Ab	0.0 ± 0.0 Ab	0.0 ± 0.0 Ab	0.0 ± 0.0 Ab	0.0 ± 0.0 Ab	16.67 B

Sensitivity of Mycogone perniciosa isolates to ozonation, based on the percentage of growth inhibition

Days	Isolate	Time of exposure						Mean

		8 h	6 h	4 h	2 h	1 h	0.5 h
4	M9	100.0 ± 0.0 Aa	100.0 ± 0.0 Aa	77.4 ± 7.1 Aab	51.60 ± 17.1 Aab	25.51 ± 11.7 Ab	0.0 ± 0.0 Ab	59.08 A

	M10	100.0 ± 0.0 Aa	100.0 ± 0.0 Aa	52.81 ± 15.0 Ab	8.18 ± 5.2 Ab	5.52 ± 3.7 Ab	0.0 ± 0.0 Ab	44.42 A

	M18	100.0 ± 0.0 Aa	100.0 ± 0.0 Aa	22.58 ± 6.5 Ab	18.22 ± 9.4 Ab	14.40 ± 3.8 Ab	0.0 ± 0.0 Ab	42.53 A

5	M9	100.0 ± 0.0 Aa	100.0 ± 0.0 Aa	69.39 ± 9.3 Aa	29.56 ± 9.5 Ab	8.32 ± 3.2 Ab	0.0 ± 0.0 Ab	51.2 A

	M10	100.0 ± 0.0 Aa	100.0 ± 0.0 Aa	36.38 ± 9.0 Ab	32.41 ± 11.5 Ab	5.20 ± 3.1 Ab	0.0 ± 0.0 Ab	45.7 A

	M18	100.0 ± 0.0 Aa	100.0 ± 0.0 Aa	14.5 ± 2.2 Ab	5.61 ± 4.9 Ab	5.01 ± 3.2 Ab	0.0 ± 0.0 Ab	37.5 A

6	M9	100.0 ± 0.0 Aa	100.0 ± 0.0 Aa	54.84 ± 6.8 Aab	44.67 ± 15.3 Aab	6.95 ± 1.7 Ab	0.0 ± 0.0 Ab	51.08 A

	M10	100.0 ± 0.0 Aa	100.0 ± 0.0 Aa	15.47 ± 8.6 Ab	8.83 ± 3.6 Ab	5.11 ± 1.3 Ab	0.0 ± 0.0 Ab	38.24 A

	M18	100.0 ± 0.0 Aa	100.0 ± 0.0 Aa	10.82 ± 8.6 Ab	8.32 ± 3.3 Ab	6.25 ± 1.3 Ab	0.0 ± 0.0 Ab	37.57 A

7	M9	100.0 ± 0.0 Aa	100.0 ± 0.0 Aa	15.45 ± 9.3 Aa	24.85 ± 8.4 Aa	0.0 ± 0.0 Aa	0.0 ± 0.0 Aa	40.05 A

	M10	100.0 ± 0.0 Aa	100.0 ± 0.0 Aa	7.5 ± 2.9 Aa	4.49 ± 2.0 Aa	0.0 ± 0.0 Aa	0.0 ± 0.0 Aa	35.33 A

	M18	100.0 ± 0.0 Aa	100 ± 0.0 Aa	7.25 ± 2.1 Ab	0.0 ± 0.0 Ab	0.0 ± 0.0 Ab	0.0 ± 0.0 Ab	34.54 A

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Efficiency of Gaseous Ozone in Disinfection of Mushroom Growing Rooms

Published Online: Dec 31, 2020

Page range: 91 - 100

Received: Mar 01, 2020

Accepted: Sep 01, 2020

DOI: https://doi.org/10.2478/johr-2020-0017

Keywords
fungal pathogens, ozonation, control

© 2020 Joanna Szumigaj-Tarnowska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Fig. 2

Fig. 3

Sensitivity of pathogenic species to ozonation based on the average rate of colonization of the plate area (%) after 5 days of incubation in relation to ozone exposure time

Sensitivity of Trichoderma aggressivum isolates to ozonation, based on the percentage of growth inhibition

List of isolates used in this study and their origin

Sensitivity of Cladobotryum dendroides isolates to ozonation, based on the percentage of growth inhibition

Sensitivity of Mycogone perniciosa isolates to ozonation, based on the percentage of growth inhibition

Efficiency of Gaseous Ozone in Disinfection of Mushroom Growing Rooms

Published Online: Dec 31, 2020

Page range: 91 - 100

Received: Mar 01, 2020

Accepted: Sep 01, 2020

DOI: https://doi.org/10.2478/johr-2020-0017

Keywordsfungal pathogens, ozonation, control

© 2020 Joanna Szumigaj-Tarnowska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Fig. 2

Fig. 3

Sensitivity of pathogenic species to ozonation based on the average rate of colonization of the plate area (%) after 5 days of incubation in relation to ozone exposure time

Sensitivity of Trichoderma aggressivum isolates to ozonation, based on the percentage of growth inhibition

List of isolates used in this study and their origin

Sensitivity of Cladobotryum dendroides isolates to ozonation, based on the percentage of growth inhibition

Sensitivity of Mycogone perniciosa isolates to ozonation, based on the percentage of growth inhibition

Keywords
fungal pathogens, ozonation, control