<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>There were two most found genera of plant parasitic nematodes from garlic plantation in Magelang, Central Java, Indonesia which suffered losses due to bulb rot, <italic>Aphelenchoides</italic> and <italic>Helicotylenchus</italic>. Polymerase Chain Reaction (PCR) was conducted using a pair of universal nematode primer (D2A/D3B) to determine the <italic>Aphelenchoides</italic> and <italic>Helicotylenchus</italic> species from those host. Both genera were amplified at ~780 bp. The Blast-N results for the <italic>Aphelenchoides</italic> showed high identity to <italic>Aphelenchoides varicaudatus</italic> from Yunnan China (HQ283353) with 99.47 % identity, while the <italic>Helicotylenchus</italic> showed 95.22 % identity to <italic>Helicotylenchus erythrinae</italic> from Colombia (MT321739). From morphological and molecular data, we confirm that the <italic>Aphelenchoides</italic> species is <italic>A. varicaudatus.</italic> Based on female morphological character, <italic>Helicotylenchus</italic> species refers to <italic>H. erythrinae</italic>. Which is also supported by its nucleotide alignment which has same region character as <italic>H. erythrinae</italic> (MT321739). This is the first report of molecular characterization of <italic>H. erythrinae</italic> in Indonesia.</p>
</abstract>

There were two most found genera of plant parasitic nematodes from garlic plantation in Magelang, Central Java, Indonesia which suffered losses due to bulb rot, Aphelenchoides and Helicotylenchus. Polymerase Chain Reaction (PCR) was conducted using a pair of universal nematode primer (D2A/D3B) to determine the Aphelenchoides and Helicotylenchus species from those host. Both genera were amplified at ~780 bp. The Blast-N results for the Aphelenchoides showed high identity to Aphelenchoides varicaudatus from Yunnan China (HQ283353) with 99.47 % identity, while the Helicotylenchus showed 95.22 % identity to Helicotylenchus erythrinae from Colombia (MT321739). From morphological and molecular data, we confirm that the Aphelenchoides species is A. varicaudatus. Based on female morphological character, Helicotylenchus species refers to H. erythrinae. Which is also supported by its nucleotide alignment which has same region character as H. erythrinae (MT321739). This is the first report of molecular characterization of H. erythrinae in Indonesia.

<italic>Aphelenchoides varicaudatus</italic> (Nematoda: Aphelenchoididae) and <italic>Helicotylenchus erythrinae</italic> (Nematoda: Hoplolaimidae) from Garlic Plantation in Magelang, Central Java, Indonesia

Department of Plant Protection, Faculty of Agriculture

Helminthologia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

 (Nematoda: Aphelenchoididae) and  (Nematoda: Hoplolaimidae) from Garlic Plantation in Magelang, Central Java, Indonesia

There were two most found genera of plant parasitic nematodes from garlic plantation in Magelang, Central Java, Indonesia which suffered losses due to bulb...

Aphelenchoides varicaudatus (Nematoda: Aphelenchoididae) and Helicotylenchus erythrinae (Nematoda: Hoplolaimidae) from Garlic Plantation in Magelang, Central Java, Indonesia

Published Online: Jun 04, 2023

Page range: 94 - 105

Received: Jun 30, 2022

Accepted: Feb 14, 2023

DOI: https://doi.org/10.2478/helm-2023-0007

Keywords
identification, molecular, morphological

© 2023 R. Maharani et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Aphelenchoides varicaudatus (Nematoda: Aphelenchoididae) and Helicotylenchus erythrinae (Nematoda: Hoplolaimidae) from Garlic Plantation in Magelang, Central Java, Indonesia

Published Online: Jun 04, 2023

Page range: 94 - 105

Received: Jun 30, 2022

Accepted: Feb 14, 2023

DOI: https://doi.org/10.2478/helm-2023-0007

Keywordsidentification, molecular, morphological

© 2023 R. Maharani et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
identification, molecular, morphological