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Diagnostic accuracy of swine echinococcosis cytopathological tests and challenges for a differential diagnosis: slaughterhouse data


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Fig. 1.

Flow chart of the diagnostic accuracy study.
Flow chart of the diagnostic accuracy study.

Fig. 2.

Kidney echinococcosis caused by Echinococcus granulosusA: Hydatid cyst in kidney, swine. B: Broad capsule of hydatid cyst with typical striated and trilaminated layer and that do not contain calcification. Note a protoscolisce (arrow). HES stain. C: Protoscolice (arrow), with inside little calcareous corpuscles (arrow head). Protoscolisce is surrounded by almost no inflammatory reaction composed of few lymphocytes and macrophages (stars). There are no neutrophils.
Kidney echinococcosis caused by Echinococcus granulosusA: Hydatid cyst in kidney, swine. B: Broad capsule of hydatid cyst with typical striated and trilaminated layer and that do not contain calcification. Note a protoscolisce (arrow). HES stain. C: Protoscolice (arrow), with inside little calcareous corpuscles (arrow head). Protoscolisce is surrounded by almost no inflammatory reaction composed of few lymphocytes and macrophages (stars). There are no neutrophils.

Fig. 3.

Diagnostic accuracy for detection of swine echinococcosis between histopathological test and cytopathological test 1 (Ziehl stain with epifluorescence microscope).
Diagnostic accuracy for detection of swine echinococcosis between histopathological test and cytopathological test 1 (Ziehl stain with epifluorescence microscope).

Fig. 4.

Diagnostic accuracy for detection of swine echinococcosis between histopathological test and cytopathological test 2 (Ziehl Neelsen stain with transmitted light microscope).
Diagnostic accuracy for detection of swine echinococcosis between histopathological test and cytopathological test 2 (Ziehl Neelsen stain with transmitted light microscope).

Fig. 5.

Cryptococcus neoformans hepatitis (5.A: HES Stain; 5.B: PAS stain: There are many budding yeasts (arrow); 5.C: Grocott Methenamine Silver stain).
Cryptococcus neoformans hepatitis (5.A: HES Stain; 5.B: PAS stain: There are many budding yeasts (arrow); 5.C: Grocott Methenamine Silver stain).

Diagnostic accuracy of cytopathological test 1 (Ziehl staining with the epifluorescence microscope) and cytopathological test 2 (Ziehl Neelsen staining with the transmitted light microscope) in the detection of clinical swine echinococcosis.

Cythopath 1% [95% CI] Cythopath 2% [95% CI] Test ratio[95% CI] p value
Specificity 100 [100 – 100] 100 [100 – 100] 1 [1 – 1]
Sensitivity 79.66[69.39 – 89.93] 66.10 [54.02 – 78.18] 1.2 [0.88 – 1.66] 0.059
PPV 100 [100 – 100] 100 [100 – 100] 1 [1 – 1]
NPV 40 [18.53 – 61.47] 28.57 [11.84 – 45.3] 1.4 [0.41 – 5.2] 0.06

Nature of infected organs in swine echinococcosis diagnosed in Côte.

Parasite Infected organs

Liver (%) Lung (%) Kidney (%)
Echinococcus granulosus 1 7 53
Echinococcus spp. 0 0 2
Total 1 (1.6) 7 (11.1) 55 (87.3)

Occurrence of diseases that could be misdiagnosed as swine echinococcosis in Côte d’Ivoire

Diseases Involved organs

Liver Lung Kidney
Ascaris suum infection or migration 4 2
Cysticercus tenuicollis hepatitis 1
Fasciolosis 1
Cryptococcus neoformans infection 1
Cirrhosis 3
Neoplasms 1(Hepatocellular carcinoma) 0 1(Small clear cell carcinoma)
Hydronephrosis 4
Actinobacillus spp or Mycoplasma spp. Pneumonia or Pleuropneumonia 11
Inflammatory conditions of unknown aetiology 2
Abscess 3

Total 11 16 7
eISSN:
1336-9083
Language:
English
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4 times per year
Journal Subjects:
Life Sciences, Zoology, Ecology, other, Medicine, Clinical Medicine, Microbiology, Virology and Infection Epidemiology