<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The study examined subpopulations of lymphocytes in peripheral blood, spleen, and jejunum including morphology of that segment in broiler chicken farm after treatment with flubendazole (Flimabend) and natural extract from chestnut wood (Farmatan). A total of 24 forty-day-old Kalimero-Super Master hybrid chickens were divided into 4 groups (n=6): the Fli group received Flimabend <italic>per os</italic>, 100 mg/g suspension in 1.43 mg of active substance/kg body weight during 7 day of experiment, Far group received Farmatan <italic>per os</italic> at 0.2 % concentration for 6 hours per day during 5 day (experimental days – from 3 to 7); the Far+Fli group received a combination of doses administered in the same way as for the first two groups; and control –C group with no active substance administration. The results demonstrated mild increase of leukocytes, lymphocytes, monocytes, leucocyte common antigen CD45, IgM+ and IgA+ cells in peripheral blood after administration of Flimabend. Similarly, subpopulations of followed lymphocytes (CD3+, CD4+, CD8+, IgM+) were increased in the jejunum after application of that drug. On the other hand, administration of Farmatan revealed opposite effect on determined immunocompetent cells what proves anti-inflammatory effect. Morphology of villi was also negatively influenced by administration of Flimabend. Administration of Farmatan suggests also its preventive administration in chickens. This tanin-containing drug as plant natural product may be used due to its antibacterial activity and as promising alternative to conventional drug with possible antihelminthic effect.</p></abstract>

The study examined subpopulations of lymphocytes in peripheral blood, spleen, and jejunum including morphology of that segment in broiler chicken farm after treatment with flubendazole (Flimabend) and natural extract from chestnut wood (Farmatan). A total of 24 forty-day-old Kalimero-Super Master hybrid chickens were divided into 4 groups (n=6): the Fli group received Flimabend per os, 100 mg/g suspension in 1.43 mg of active substance/kg body weight during 7 day of experiment, Far group received Farmatan per os at 0.2 % concentration for 6 hours per day during 5 day (experimental days – from 3 to 7); the Far+Fli group received a combination of doses administered in the same way as for the first two groups; and control –C group with no active substance administration. The results demonstrated mild increase of leukocytes, lymphocytes, monocytes, leucocyte common antigen CD45, IgM+ and IgA+ cells in peripheral blood after administration of Flimabend. Similarly, subpopulations of followed lymphocytes (CD3+, CD4+, CD8+, IgM+) were increased in the jejunum after application of that drug. On the other hand, administration of Farmatan revealed opposite effect on determined immunocompetent cells what proves anti-inflammatory effect. Morphology of villi was also negatively influenced by administration of Flimabend. Administration of Farmatan suggests also its preventive administration in chickens. This tanin-containing drug as plant natural product may be used due to its antibacterial activity and as promising alternative to conventional drug with possible antihelminthic effect.

The influence of chestnut wood and flubendazole on morphology of small intestine and lymphocytes of peripheral blood, spleen and jejunum in broiler chickens

Helminthologia

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The study examined subpopulations of lymphocytes in peripheral blood, spleen, and jejunum including morphology of that segment in broiler chicken farm after...

Peripheral blood
Groups	Subpopulations of lymphocytes (mean ± SD)
	CD3	CD4	CD8	IgM	IgA	CD45
C	2.33 ± 0.79	1.32 ± 0.44	0.80 ± 0.20	0.85 ± 0.24	0.29 ± 0.26	4.32 ± 0.62
Far	2.37 ± 1.08	1.45 ± 0.63	0.72 ± 0.37	0.58 ± 0.23^a	0.13 ± 0.11^c	2.89 ± 1.01^a
Fli	3.12 ± 0.90	1.82 ± 0.71	0.84 ± 0.19	1.20 ± 0.32^c	0.70 ± 0.39^a	5.42 ± 1.05^d
Far+Fli	3.05 ± 0.73	1.99 ± 0.69	0.75 ± 0.18	0.95 ± 0.22	0.26 ± 0.12^c	5.18 ± 0.84^d

WBC	C	Far	Fli	Far+Fli
Leukocytes	13.72 ± 2.71	8.85 ± 2.92^a	14.58 ± 3.02^b	14.40 ± 3.46^b
Lymphocytes	6.68 ± 0.35	5.44 ± 1.57^a	8.63 ± 1.53^d	8.37 ± 0.72^d
Heterophiles	6.23 ± 2.44	2.65 ± 1.41	4.91 ± 2.39	4.63 ± 3.18
Eosinophiles	0.53 ± 0.23	0.47 ± 0.19	0.61 ± 0.34	0.88 ± 0.34
Monocytes	0.43 ± 0.06	0.29 ± 0.07^a	0.44 ± 0.14	0.46 ± 0.12^b

Subpopulations	C	Far	Fli	Far+Fli
CD3	598.90 ± 204.20^d	312.00 ± 105.10^a	680.10 ± 186.80^d	672.30 ± 252.00^d
CD4	614.40 ± 187.00^d	282.00 ± 94.69^a	641.70 ± 185.40^da	547.10 ± 151.70^dc
CD8	770.10 ± 213.40^d	388.60 ± 159.50^a	794.20 ± 257.30^d	743.20 ± 220.20^d
IgM	203.90 ± 122.30^a	146.70 ± 91.80^c	216.00 ± 88.14^a	149.60 ± 73.22^bc
IgA	331.10 ± 162.40	250.80 ± 180.40	294.10 ± 200.20	274.60 ± 160.40

The influence of chestnut wood and flubendazole on morphology of small intestine and lymphocytes of peripheral blood, spleen and jejunum in broiler chickens

Published Online: Nov 06, 2019

Page range: 273 - 281

Received: Jan 31, 2019

Accepted: Aug 25, 2019

DOI: https://doi.org/10.2478/helm-2019-0029

Keywords
Farmatan, Flubendazol, chicken, immunity, intestine

© 2019 M. Levkut , V. Revajová, M. Levkutová, E. Selecká, Z. Ševčíková, V. Karaffová, M. Levkut, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Fig. 5

Fig. 6

Subpopulations of lymphocytes in the peripheral blood (total counts = G.L–1) and spleen (relative percentage)(mean ± SD).

Absolute count of white blood cells (WBC; G.L–1= 109. L–1) in the peripheral blood of chickens (mean ± SD).

Subpopulations of lymphocytes in jejunal mucosa (mm2).

The influence of chestnut wood and flubendazole on morphology of small intestine and lymphocytes of peripheral blood, spleen and jejunum in broiler chickens

Published Online: Nov 06, 2019

Page range: 273 - 281

Received: Jan 31, 2019

Accepted: Aug 25, 2019

DOI: https://doi.org/10.2478/helm-2019-0029

KeywordsFarmatan, Flubendazol, chicken, immunity, intestine

© 2019 M. Levkut , V. Revajová, M. Levkutová, E. Selecká, Z. Ševčíková, V. Karaffová, M. Levkut, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Fig. 1

Fig. 2

Fig. 3

Fig. 4

Fig. 5

Fig. 6

Subpopulations of lymphocytes in the peripheral blood (total counts = G.L–1) and spleen (relative percentage)(mean ± SD).

Absolute count of white blood cells (WBC; G.L–1= 109. L–1) in the peripheral blood of chickens (mean ± SD).

Subpopulations of lymphocytes in jejunal mucosa (mm2).

Keywords
Farmatan, Flubendazol, chicken, immunity, intestine