Open Access

Effect of heat acclimation on thermotolerance of in vitro strawberry plantlets

Zevon Julio Seymour
Zevon Julio Seymour
, 
Juan Francisco Mercedes
Juan Francisco Mercedes
 and 
Jong-Yi Fang
Jong-Yi Fang
   | Apr 24, 2024
Folia Horticulturae's Cover Image
About this article
Previous Article
Next Article
Cite
Article Category: ORIGINAL ARTICLE
Published Online: Apr 24, 2024
Page range: -
Received: Aug 07, 2023
Accepted: Feb 28, 2024
DOI: https://doi.org/10.2478/fhort-2024-0008
Keywords
© 2024 Zevon Julio Seymour et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1.

Overview of the control, heat acclimation and non-acclimation treatments used in this study.
Overview of the control, heat acclimation and non-acclimation treatments used in this study.

Figure 2.

Morphology of strawberry plantlets 21 days after lethal temperature exposure. (A) T0 plantlet with healthy green leaves. (B) T3 plantlet with deterioration of original leaves and emergence of new leaves. (C) TN plantlet with deterioration of original leaves without new leaf growth. Bar = 1 cm.
Morphology of strawberry plantlets 21 days after lethal temperature exposure. (A) T0 plantlet with healthy green leaves. (B) T3 plantlet with deterioration of original leaves and emergence of new leaves. (C) TN plantlet with deterioration of original leaves without new leaf growth. Bar = 1 cm.

Figure 3.

Activity of SOD (A), APX (B), GR (C), CAT (D) and POD (E) of strawberry plantlets following heat acclimation and lethal heat stress from 25°C to 48°C. Values are mean ± SE. Values followed by different letters are significantly different at p < 0.05, separated by Duncan’s multiple range test. APX, ascorbate peroxidase; CAT, catalase; GR, glutathione reductase; POD, peroxidase; SOD, superoxide dismutase.
Activity of SOD (A), APX (B), GR (C), CAT (D) and POD (E) of strawberry plantlets following heat acclimation and lethal heat stress from 25°C to 48°C. Values are mean ± SE. Values followed by different letters are significantly different at p < 0.05, separated by Duncan’s multiple range test. APX, ascorbate peroxidase; CAT, catalase; GR, glutathione reductase; POD, peroxidase; SOD, superoxide dismutase.

Figure 4.

Antioxidant enzyme activities of the strawberry plantlets following the control (T0), heat-acclimation treatment (T3) and non-acclimation treatment (TN). (A) SOD, (B) APX, (C) GR, (D) CAT and (E) POD. Values are means ± SE. Values followed by different letters are significantly different at p < 0.05, separated by Duncan’s multiple range test. APX, ascorbate peroxidase; CAT, catalase; GR, glutathione reductase; POD, peroxidase; SOD, superoxide dismutase.
Antioxidant enzyme activities of the strawberry plantlets following the control (T0), heat-acclimation treatment (T3) and non-acclimation treatment (TN). (A) SOD, (B) APX, (C) GR, (D) CAT and (E) POD. Values are means ± SE. Values followed by different letters are significantly different at p < 0.05, separated by Duncan’s multiple range test. APX, ascorbate peroxidase; CAT, catalase; GR, glutathione reductase; POD, peroxidase; SOD, superoxide dismutase.

Survival rates of heat-acclimated strawberry plantlets at different days after lethal heat stress.

Treatment 7 days 14 days 21 days 28 days
T0 100 a 100 a 100 a 100 a
T1 55 ± 0.31 b 55 ± 0.10 b 75 ± 0.36 b 75 ± 0.36 b
T2 55 ± 0.10 b 60 ± 0.00 b 85 ± 0.36 b 95 ± 0.32 ab
T3 70 ± 0.00 b 85 ± 0.58 ab 95 ± 0.14 ab 100 a
T4 80 ± 0.26 b 95 ± 0.32 a 100 a 100 a
TN 0 c 0 c 0 c 0 c

Number of new adventitious roots produced by heat-acclimated strawberry plantlets at different days after lethal heat stress.

Treatment 7 Days 14 Days 21 Days 28 Days 35 Days 42 Days 49 Days 56 Days
T0 1.40 ± 0.40 a 2.70 ± 0.56 a 4.10 ± 0.72 a 5.00 ± 0.98 a 5.70 ± 1.09 a 6.40 ± 1.28 a 6.50 ± 1.32 a 6.80 ± 1.31 a
T1 0 0 0.40 ± 0.31 bc 1.10 ± 0.46 bc 1.10 ± 0.46 cd 1.80 ± 0.55 cd 2.90 ± 0.80 b 3.10 ± 0.77 b
T2 0 0.30 ± 0.21 bc 0.60 ± 0.31 bc 1.40 ± 0.69 bc 1.50 ± 0.67 cd 2.10 ± 0.64 c 2.60 ± 0.82 b 3.00 ± 0.84 b
T3 0.40 ± 0.27 b 0.90 ± 0.28 b 1.60 ± 0.37 b 2.60 ± 0.50 b 3.70 ± 0.50 b 4.40 ± 0.50 b 4.70 ± 0.50 ab 4.80 ± 0.49 ab
T4 0 0.40 ± 0.22 bc 1.30 ± 0.47 b 1.80 ± 0.44 bc 2.90 ± 0.43 bc 3.20 ± 0.51 bc 3.80 ± 0.61 b 3.90 ± 0.60 b

Number of new leaves produced by heat-acclimated strawberry plantlets at different days after lethal heat stress.

Treatment 7 Days 14 Days 21 Days 28 Days 35 Days 42 Days 49 Days 56 Days
T0 0.80 ± 0.13 a 1.30 ± 0.21 ab 2.20 ± 0.20 b 3.10 ± 0.18 c 4.20 ± 0.20 b 5.10 ± 0.23 b 5.70 ± 0.15 a 6.00 ± 0.21 a
T1 0.10 ± 0.10 c 1.00 ± 0.33 b 2.10 ± 0.43 b 3.50 ± 0.45 c 4.40 ± 0.56 b 5.00 ± 0.65 b 5.80 ± 0.74 a 6.30 ± 0.83 a
T2 0 1.30 ± 0.42 ab 3.50 ± 0.43 a 4.80 ± 0.42 a 6.00 ± 0.54 a 6.50 ± 0.45 a 6.60 ± 0.43 a 6.60 ± 0.43 a
T3 0.40 ± 0.16 b 2.10 ± 0.31 a 3.50 ± 0.40 a 4.60 ± 0.54 ab 5.20 ± 0.51 ab 5.70 ± 0.45 ab 6.50 ± 0.40 a 7.40 ± 0.45 a
T4 0.40 ± 0.16 b 1.50 ± 0.22 ab 2.50 ± 0.22 b 3.70 ± 0.30 bc 4.60 ± 0.27 b 5.40 ± 0.27 ab 6.00 ± 0.26 a 6.70 ± 0.33 a

Number of new lateral roots produced by heat-acclimated strawberry plantlets at different days after lethal heat stress.

Treatment 7 Days 14 Days 21 Days 28 Days 35 Days 42 Days 49 Days 56 Days
T0 0 0.80 ± 0.51bc 1.20 ± 0.76 cd 1.50 ± 0.82 c 1.50 ± 0.82 c 1.50 ± 0.82 cd 1.50 ± 0.82 cd 1.50 ± 0.82 c
Tl 0 0.60 ± 0.50 bc 1.80 ± 0.94 bcd 2.40 ± 1.09 bc 2.40 ± 1.09 bc 2.90 ± 1.15 bc 3.50 ± 1.22 bc 4.60 ± 1.53 b
T2 0.50 ± 0.50 a 1.60 ± 1.08 abc 3.20 ± 1.44 abc 4.50 ± 1.27 ab 4.70 ± 1.21 ab 5.10 ± 1.22 ab 5.60 ± 1.14 ab 6.10 ± 1.22 ab
T3 0 2.20 ± 0.66 ab 4.80 ± 0.76 a 5.60 ± 0.81 a 5.70 ± 0.80 a 6.30 ± 0.78 a 7.00 ± 0.76 a 7.20 ± 0.79 ab
T4 0.20 ± 0.20 a 3.00 ± 0.67 a 4.20 ± 0.79 ab 4.50 ± 0.89 ab 5.10 ± 0.81 a 6.50 ± 0.64 a 7.40 ± 0.52 a 7.90 ± 0.38 a

Chlorophylls, electrolyte leakage and free proline content of heat-acclimated strawberry plantlets after lethal heat stress.

Treatment Chlorophyll (mg · g-1 FW) Electrolyte leakage (%) Proline (mg · g-1 FW)
Total Ch a Ch b
T0 3.92 ± 0.02 a 2.81 ± 0.02 a 1.11 ± 0.04 a 10.76 ± 0.04 c 1.38 ± 0.41 b
T3 1.81 ± 0.04 b 1.26 ± 0.02 b 0.54 ± 0.03 b 22.46 ± 0.70 b 3.34 ± 0.13 a
TN 1.11 ± 0.01 c 0.75 ± 0.01 c 0.36 ± 0.02 c 38.26 ± 0.62 a 1.69 ± 0.09 b
eISSN:
2083-5965
Language:
English
Publication timeframe:
2 times per year
Journal Subjects:
Life Sciences, Plant Science, Zoology, Ecology, other
Journal RSS Feed