No-tillage (UT) and tillage (TL) influence melon (Cucumis melo L.) production. However, the mechanism of improving the soil quality under UT in melon production is still unavailable. In this study, we attempted to explore the effects of UT and TL treatments on soil fertility and the microbial abundance and diversity in planting melon under greenhouse condition. Soil properties were determined and the bacterial v4-v5 16S rRNA and the fungal internal transcribed spacer gene were pyrosequenced by extracting greenhouse soil DNA. Results showed that the two treatments had different effects on nutrient uptake in melon plants under facility conditions. Additional nitrogen (N) was absorbed in the leaves and fruit in UT treatment. However, the N content in the UT treatment was kept as similar to that of the TL treatment. The phosphorus (P) contents in melon plant leaves and fruits in the UT treatment were higher than those in the TL treatment. High potassium (K) contents were observed in fruits and melon stem under the UT and TL treatments, respectively. Soil pH, organic matter and the available N influenced the bacterial and fungal distributions. The total N, total P and total K in melon plants were correlated with the bacterial and fungal groups in facility soils. The UT treatment had a substantial effect on the microbial diversity in soils planted with melon. Our study provided insights into the response of soil fertility and microbial structures to UT and TL treatments under greenhouse soils, which may aid in managing greenhouse soil quality.
