Additional records of 28 species of interest belonging to acalyptrate families Lonchaeidae (1 species), Lauxaniidae (2 species), Chamaemyiidae (9 species), Agromyzidae (16 species) and Opomyzidae (1 species) from glacial sand deposits in the Czech Silesia (NE Czech Republic) are presented and their association with sandy habitats discussed. A total of 25 species are recorded from the Czech Silesia for the first time, 15 are new additions to the fauna of Moravia of which 11 are also new for the whole of the Czech Republic. Six species (Lauxaniidae 1, Agromyzidae 5) are new additions to the Diptera fauna of Poland. Leucopis cerasiphila Gomolitzkaja & Tanasijtshuk, 1986 is recorded for the first time from Europe. None of the here reported species is classified as psammobiont but 8 species are considered psammophilous or probably psammophilous, viz. Minettia longiseta (Loew, 1847) (Lauxaniidae), Leucopis kaszabi Tanasijtshuk, 1970 (Chamaemyiidae), Cerodontha (Xenophytomyza) leptophallus Papp, 2016, Hexomyza sarothamni (Hendel, 1923), Liriomyza dracunculi Hering, 1932, L. latigenis (Hendel, 1920), L. yasumatsui Sasakawa, 1972 (Agromyzidae) and Geomyza subnigra Drake, 1992 (Opomyzidae). The conspicuous species diversity of Chamaemyiidae in Silesian glacial sand deposits is discussed.
