<abstract xmlns="http://www.w3.org/1999/xhtml">In the present study we tested the dose andh time dependence of the antinematodal effects of carvacrol and tyhmol on <italic>Caenorabditis elegans,</italic> and the efficacy of carvacrol, thymol, p-cymene and cinnamaldehyde,which were administrated in the drinking water of rats naturally infected with the pinworm <italic>Syphacia muris</italic>. The control treatment of the infected rats was carried out with piperazine. Thymol caused a dose and time-dependent mortality in adult <italic>C. elegans</italic>. The value of the Median Lethal Concentration (LC50) of thymol was 117.9nM after 24h and 62.89 nM after 48h of exposure. Carvacrol exhibited a higher antinematodal efficiency than thymol. The LC50 of carvacrol, after 24 hours of exposure, was 53.03 nM, while after 48 hours it was 33.83 nM. On the other hand, piperazine showed an extremely high efficacy against <italic>S. muris</italic> infection in rats. Piperazine, at a dose of 625 mg/kg bw, administered in drinking water continuously for 10 days, eliminates the infection completely. However, none of the investigated active ingredients of essential oils were effective against <italic>S. muris</italic>. The reason for the lack of efficiency may be due to their pharmacokinetic properties. A relatively low amount of, orally administered, active ingredients of essential oils reaches the distal segments of the gastrointestinal tract, where <italic>S. muris</italic> inhabits the gut (colon and cecum). The obtained results, on <italic>C. elegans</italic>, indicate a clear dose and time-dependent antinematodal effect of thymol and carvacrol. However, for clinical application, it is necessary to examine the efficacy of microencapsulated formulations with a controlled release of active ingredients of essential oils in certain parts of the gastrointestinal tract.</abstract>

In the present study we tested the dose andh time dependence of the antinematodal effects of carvacrol and tyhmol on Caenorabditis elegans, and the efficacy of carvacrol, thymol, p-cymene and cinnamaldehyde,which were administrated in the drinking water of rats naturally infected with the pinworm Syphacia muris. The control treatment of the infected rats was carried out with piperazine. Thymol caused a dose and time-dependent mortality in adult C. elegans. The value of the Median Lethal Concentration (LC50) of thymol was 117.9nM after 24h and 62.89 nM after 48h of exposure. Carvacrol exhibited a higher antinematodal efficiency than thymol. The LC50 of carvacrol, after 24 hours of exposure, was 53.03 nM, while after 48 hours it was 33.83 nM. On the other hand, piperazine showed an extremely high efficacy against S. muris infection in rats. Piperazine, at a dose of 625 mg/kg bw, administered in drinking water continuously for 10 days, eliminates the infection completely. However, none of the investigated active ingredients of essential oils were effective against S. muris. The reason for the lack of efficiency may be due to their pharmacokinetic properties. A relatively low amount of, orally administered, active ingredients of essential oils reaches the distal segments of the gastrointestinal tract, where S. muris inhabits the gut (colon and cecum). The obtained results, on C. elegans, indicate a clear dose and time-dependent antinematodal effect of thymol and carvacrol. However, for clinical application, it is necessary to examine the efficacy of microencapsulated formulations with a controlled release of active ingredients of essential oils in certain parts of the gastrointestinal tract.

Antihelminic Activity of Carvacrol, Thymol, Cinnamaldehyde and P-Cymen Against the Free-Living Nematode <italic>Caenorhabditis elegans</italic> and Rat Pinworm <italic>Syphacia muris</italic>

Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine

Department of Parasitology, Faculty of Veterinary Medicine

Department of Pathology, Faculty of Veterinary Medicine

Scientific Institute of Veterinary Medicine of Serbia

Department of Reproduction, Fertility and Artificial Insemination, Faculty of Veterinary Medicine

Acta Veterinaria

This work is licensed under the Creative Commons Attribution 4.0 International License.

Antihelminic Activity of Carvacrol, Thymol, Cinnamaldehyde and P-Cymen Against the Free-Living Nematode  and Rat Pinworm 

In the present study we tested the dose andh time dependence of the antinematodal effects of carvacrol and tyhmol on Caenorabditis elegans, and the efficacy of...

Antihelminic Activity of Carvacrol, Thymol, Cinnamaldehyde and P-Cymen Against the Free-Living Nematode Caenorhabditis elegans and Rat Pinworm Syphacia muris

Published Online: Jan 01, 2019

Page range: 445 - 456

Received: Sep 03, 2018

Accepted: Nov 23, 2018

DOI: https://doi.org/10.2478/acve-2018-0036

Keywords
carvacrol, thymol, piperazine

© 2018 Marjanović S. Đorđe et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Antihelminic Activity of Carvacrol, Thymol, Cinnamaldehyde and P-Cymen Against the Free-Living Nematode Caenorhabditis elegans and Rat Pinworm Syphacia muris

Published Online: Jan 01, 2019

Page range: 445 - 456

Received: Sep 03, 2018

Accepted: Nov 23, 2018

DOI: https://doi.org/10.2478/acve-2018-0036

Keywordscarvacrol, thymol, piperazine

© 2018 Marjanović S. Đorđe et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
carvacrol, thymol, piperazine