<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Bladder cancer (BCa) is one of the most common urinary cancers. The present study aims to investigate whether Paeoniflorin (Pae) can exert inhibitory effects on BCa. The results showed that Pae inhibited proliferation of human BCa cell lines in a concentration- and time-dependent manner. Pae and cisplatin (Cis) synergistically inhibited the growth of tumours in RT4-bearing mice. Pae treatment neutralized the body loss induced by Cis. Moreover, Pae induced apoptosis in RT4 cells and increased the activities of caspase3, caspase8 and caspase9. Western blotting and immunohistochemical analysis revealed that the phosphorylated signal transducer and activator of transcription-3 (p-STAT3) level were decreased in Pae-treated RT4 cells and Pae-treated tumour-bearing mice. Furthermore, STAT3 transcriptional target B-cell lymphoma-2 was decreased in Pae-treated RT4 cells. Interestingly, Pae prevented translocation of STAT3 to the nucleus in RT4 cells. Collectively, Pae inhibits the growth of BCa, at least in part, <italic>via</italic> a STAT3 pathway.</p></abstract>

Bladder cancer (BCa) is one of the most common urinary cancers. The present study aims to investigate whether Paeoniflorin (Pae) can exert inhibitory effects on BCa. The results showed that Pae inhibited proliferation of human BCa cell lines in a concentration- and time-dependent manner. Pae and cisplatin (Cis) synergistically inhibited the growth of tumours in RT4-bearing mice. Pae treatment neutralized the body loss induced by Cis. Moreover, Pae induced apoptosis in RT4 cells and increased the activities of caspase3, caspase8 and caspase9. Western blotting and immunohistochemical analysis revealed that the phosphorylated signal transducer and activator of transcription-3 (p-STAT3) level were decreased in Pae-treated RT4 cells and Pae-treated tumour-bearing mice. Furthermore, STAT3 transcriptional target B-cell lymphoma-2 was decreased in Pae-treated RT4 cells. Interestingly, Pae prevented translocation of STAT3 to the nucleus in RT4 cells. Collectively, Pae inhibits the growth of BCa, at least in part, via a STAT3 pathway.

Paeoniflorin inhibits the growth of bladder carcinoma <italic>via</italic> deactivation of STAT3

Urology and Nephrology Institute of Ningbo University

Acta Pharmaceutica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Paeoniflorin inhibits the growth of bladder carcinoma  deactivation of STAT3

Bladder cancer (BCa) is one of the most common urinary cancers. The present study aims to investigate whether Paeoniflorin (Pae) can exert inhibitory effects...

Paeoniflorin inhibits the growth of bladder carcinoma via deactivation of STAT3

Published Online: Apr 26, 2018

Page range: 211 - 222

Accepted: Dec 20, 2017

DOI: https://doi.org/10.2478/acph-2018-0013

Keywords
bladder cancer, Radix, paeoniflorin, apoptosis, STAT3

© 2018 Jianhui-Yang et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Paeoniflorin inhibits the growth of bladder carcinoma via deactivation of STAT3

Published Online: Apr 26, 2018

Page range: 211 - 222

Accepted: Dec 20, 2017

DOI: https://doi.org/10.2478/acph-2018-0013

Keywordsbladder cancer, Radix, paeoniflorin, apoptosis, STAT3

© 2018 Jianhui-Yang et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
bladder cancer, Radix, paeoniflorin, apoptosis, STAT3