<abstract xmlns="http://www.w3.org/1999/xhtml"><p> For the first time, nuclear microsatellite (nSSRs) primers were developed in the endangered tree species Lecythis ampla (Lecythidaceae) as molecular tools. An enrichment protocol with genomic DNA libraries for nSSRs was used to identify candidate loci. A large number of candidate loci were identified. Consecutively population genetic parameters of these loci were tested in two available populations. Eventually 17 microsatellite loci have been identified that show no or only low evidence for linkage disequilibrium, deviations from Hardy-Weinberg expectations or high levels of null alleles. These markers are apt for future molecular population studies.</p></abstract>

For the first time, nuclear microsatellite (nSSRs) primers were developed in the endangered tree species Lecythis ampla (Lecythidaceae) as molecular tools. An enrichment protocol with genomic DNA libraries for nSSRs was used to identify candidate loci. A large number of candidate loci were identified. Consecutively population genetic parameters of these loci were tested in two available populations. Eventually 17 microsatellite loci have been identified that show no or only low evidence for linkage disequilibrium, deviations from Hardy-Weinberg expectations or high levels of null alleles. These markers are apt for future molecular population studies.

Novel nuclear microsatellites in the endangered neotropical timber tree Lecythis ampla (Lecythidaceae)

Silvae Genetica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

For the first time, nuclear microsatellite (nSSRs) primers were developed in the endangered tree species Lecythis ampla (Lecythidaceae) as molecular tools. An...

Novel nuclear microsatellites in the endangered neotropical timber tree Lecythis ampla (Lecythidaceae)

Published Online: Jun 01, 2017

Page range: 181 - 184

Received: Jul 28, 2014

DOI: https://doi.org/10.1515/sg-2014-0023

Keywords
endangered species, genetic diversity, Lecythis ampla, Lecythidaceae, microsatellites

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Novel nuclear microsatellites in the endangered neotropical timber tree Lecythis ampla (Lecythidaceae)

Published Online: Jun 01, 2017

Page range: 181 - 184

Received: Jul 28, 2014

DOI: https://doi.org/10.1515/sg-2014-0023

Keywordsendangered species, genetic diversity, Lecythis ampla, Lecythidaceae, microsatellites

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
endangered species, genetic diversity, Lecythis ampla, Lecythidaceae, microsatellites