From primer design to validation of results - is it possible by using free software only? / De la proiectarea primerilor la validarea rezultatelor - este posibil utilizând doar programe gratuite?

1. PrimerBank [Internet]. [cited 2015 May 6]. Available from: http://pga.mgh.harvard.edu/primerbank/Search in Google Scholar

2. RTPrimerDB: the real-time PCR primer and probe database - Homepage [Internet]. [cited 2015 May 6]. Available from: http://medgen.ugent.be/rtprimerdb/index. phpSearch in Google Scholar

3. qPrimerDepot [Internet]. [cited 2015 May 6]. Available from: http://primerdepot.nci.nih.gov/Search in Google Scholar

4. Primer designing tool [Internet]. [cited 2015 May 6]. Available from: http://www.ncbi.nlm.nih.gov/tools/primer-blast/Search in Google Scholar

5. Primer3 Input [Internet]. [cited 2015 May 6]. Available from: http://biotools.umassmed.edu/bioapps/primer3_ www.cgiSearch in Google Scholar

6. National Center for Biotechnology Information [Internet]. [cited 2015 May 6]. Available from: http://www.ncbi.nlm.nih.gov/Search in Google Scholar

7. Tm Calculator | Life Technologies [Internet]. [cited 2015 May 30]. Available from: https://www.lifetechnologies.com/ch/en/home/brands/thermo-scientific/ molecular-biology/molecular-biology-learning-center/ molecular-biology-resource-library/thermo-scientific- web-tools/tm-calculator.htmlSearch in Google Scholar

8. Melting Temperature (Tm) Calculation [Internet]. [cited 2015 May 6]. Available from: http://www.biophp.org/minitools/melting_temperature/demo.phpSearch in Google Scholar

9. Tm Calculator [Internet]. [cited 2015 May 30]. Available from: http://appliedbiosystem.com/support/techtools/calc/Search in Google Scholar

10. Beacon Designer Free Edition [Internet]. [cited 2015 May 6]. Available from: http://www.premierbiosoft.com/qOligo/Oligo.jsp?PID=1Search in Google Scholar

11. Mathews Lab RNAstructure Web Servers [Internet]. [cited 2015 May 6]. Available from: http://rna.urmc.rochester.edu/RNAstructureWeb/index.htmlSearch in Google Scholar

12. Serial Cloner [Internet]. [cited 2015 May 7]. Available from: http://serialbasics.free.fr/Serial_Cloner.htmlSearch in Google Scholar

13. Technelysium | Chromas [Internet]. [cited 2015 May 7]. Available from: http://technelysium.com.au/?page_ id=13Search in Google Scholar

• Normality assessment, few paradigms and use cases

• Novel DCX pathogenic variant in a girl with subcortical band heterotopia

• Expression of serum soluble Klotho protein in patients with renal damage induced by anti-neutrophil cytoplasmic antibody-associated small-vessel vasculitis and influence on prognosis

• Expressions of TGF-β1 and VEGF in patients with acute myeloid leukemia and associations with prognosis

• Carbapenem resistance determinants in Klebsiella pneumoniae strains isolated from blood cultures-comparative analysis of molecular and phenotypic methods

• SPP1 is a biomarker of cervical cancer prognosis and involved in immune infiltration

• Elbow Septic Arthritis caused by Klebsiella pneumoniae in a Newborn - Case Report and Literature Review

• Downregulation of hsa-miR-4328 and target gene prediction in Acute Promyelocytic Leukemia

• Antibacterial Activity of Clove, Oregano, Thyme, Eucalyptus, and Tea Tree Essential Oils against Escherichia coli and Klebsiella pneumoniae strains

• Associations of serum vitamin D and Fok I polymorphism of receptor gene with unexplained recurrent spontaneous abortion