<abstract xmlns="http://www.w3.org/1999/xhtml"> Escherichia coli O157 related foodborne illnesses continue to be one of the most important global public health problems in the world. This study aims to determine E. coli O157 prevalence in 375 chicken meat parts and giblets. The samples were collected randomly from several supermarkets and butchers in Diyarbakir, a city in southeast Turkey. They were analyzed and confirmed using the immunomagnetic separation (IMS), Vitek® 2 microbial identification system and polymerase chain reaction (PCR) method. This study also aims to detect the presence of fliCH7, eaeA, stx1, stx2 and hlyA genes by using PCR. The overall E. coli O157 prevalence in chicken meat parts and giblets was 1.3%. All of the E. coli O157 isolates carried rfbEO157 and eaeA genes; but not any fliCH7 and hlyA genes. The E. coli O157 isolates obtained from drumstick and breast meat carried either stx1 or stx2 genes, which were related to important virulence factors of the disease.</abstract>

Escherichia coli O157 related foodborne illnesses continue to be one of the most important global public health problems in the world. This study aims to determine E. coli O157 prevalence in 375 chicken meat parts and giblets. The samples were collected randomly from several supermarkets and butchers in Diyarbakir, a city in southeast Turkey. They were analyzed and confirmed using the immunomagnetic separation (IMS), Vitek® 2 microbial identification system and polymerase chain reaction (PCR) method. This study also aims to detect the presence of fliCH7, eaeA, stx1, stx2 and hlyA genes by using PCR. The overall E. coli O157 prevalence in chicken meat parts and giblets was 1.3%. All of the E. coli O157 isolates carried rfbEO157 and eaeA genes; but not any fliCH7 and hlyA genes. The E. coli O157 isolates obtained from drumstick and breast meat carried either stx1 or stx2 genes, which were related to important virulence factors of the disease.

Prevalence and some virulence genes of Escherichia coli O157 isolated from chicken meats and giblets

Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Dicle University, 21280, Diyarbakir,

Annals of Animal Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Escherichia coli O157 related foodborne illnesses continue to be one of the most important global public health problems in the world. This study aims to...

Prevalence and some virulence genes of Escherichia coli O157 isolated from chicken meats and giblets

Article Category: Quality and safety of animal origin products

Published Online: Apr 28, 2017

Page range: 555 - 563

Received: Jul 25, 2016

Accepted: Oct 04, 2016

DOI: https://doi.org/10.1515/aoas-2016-0061

Keywords
E. coli O157, chicken meat, stx1, stx2, eaeA, hlyA

© by Husnu Sahan Guran

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Prevalence and some virulence genes of Escherichia coli O157 isolated from chicken meats and giblets

Article Category: Quality and safety of animal origin products

Published Online: Apr 28, 2017

Page range: 555 - 563

Received: Jul 25, 2016

Accepted: Oct 04, 2016

DOI: https://doi.org/10.1515/aoas-2016-0061

KeywordsE. coli O157, chicken meat, stx1, stx2, eaeA, hlyA

© by Husnu Sahan Guran

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
E. coli O157, chicken meat, stx1, stx2, eaeA, hlyA