<abstract xmlns="http://www.w3.org/1999/xhtml"> An in vitro gas production technique, using rumen fluid from four Kacang × Boer crossbred adult goats was used to study the effects of commercial tannic acid (TA, a hydrolysable tannin) on methanogenesis, fatty acid composition and biohydrogenation (BH) of C18 polyunsaturated fatty acids (PUFA) in the rumen. Treatments were control (CON, 50% alfalfa hay (AH) + 50% concentrate), 25 mg TA/250 mgDM (LTA, low TA) and 50 mg TA/250 mgDM (HTA, High TA), which were mixed with 30 mL of buffered rumen fluid and incubated for 24 h. The study revealed that TA supplementation had no negative effect on rumen fermentation parameters such as pH, NH3N, acetic/propionic ratio and total volatile fatty acid (tVFA). Methane (CH4) production (mL/250 mg DM) decreased (P&lt;0.05) with increasing levels of TA. Greatest CH4 reduction (%) was recorded for MTA (20.30%) and LTA (13.00%) compared with CON. Supplementation of the diet with TA did not affect the rate of rumen BH (%) of C18:1n-9 (oleic acid; OA), C18:2n-6 (linoleic acid; LA), C18:3n-3 (linolenic acid; LNA) and the concentration of fatty acids after 24 h of in vitro incubation. Based on this study, the addition of TA in vitro reduced rumen methanogenesis without negative effect of rumen fermentation characteristics, but in vivo studies need to be performed to determine if concentrations that inhibit methane are below toxic levels.</abstract>

An in vitro gas production technique, using rumen fluid from four Kacang × Boer crossbred adult goats was used to study the effects of commercial tannic acid (TA, a hydrolysable tannin) on methanogenesis, fatty acid composition and biohydrogenation (BH) of C18 polyunsaturated fatty acids (PUFA) in the rumen. Treatments were control (CON, 50% alfalfa hay (AH) + 50% concentrate), 25 mg TA/250 mgDM (LTA, low TA) and 50 mg TA/250 mgDM (HTA, High TA), which were mixed with 30 mL of buffered rumen fluid and incubated for 24 h. The study revealed that TA supplementation had no negative effect on rumen fermentation parameters such as pH, NH3N, acetic/propionic ratio and total volatile fatty acid (tVFA). Methane (CH4) production (mL/250 mg DM) decreased (P<0.05) with increasing levels of TA. Greatest CH4 reduction (%) was recorded for MTA (20.30%) and LTA (13.00%) compared with CON. Supplementation of the diet with TA did not affect the rate of rumen BH (%) of C18:1n-9 (oleic acid; OA), C18:2n-6 (linoleic acid; LA), C18:3n-3 (linolenic acid; LNA) and the concentration of fatty acids after 24 h of in vitro incubation. Based on this study, the addition of TA in vitro reduced rumen methanogenesis without negative effect of rumen fermentation characteristics, but in vivo studies need to be performed to determine if concentrations that inhibit methane are below toxic levels.

An in vitro study on the ability of tannic acid to inhibit methanogenesis and biohydrogenation of C18 PUFA in the rumen of goats

Department of Veterinary Preclinical Sciences, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia

Department of Animal Resources, College of Agriculture, University of Al-Qassim Green, Babylon, 51013 Al-Qassim,

Department of Veterinary Preclinical Sciences, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan,

Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan,

Annals of Animal Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

An in vitro gas production technique, using rumen fluid from four Kacang × Boer crossbred adult goats was used to study the effects of commercial tannic acid...

An in vitro study on the ability of tannic acid to inhibit methanogenesis and biohydrogenation of C18 PUFA in the rumen of goats

Article Category: Animal nutrition, and feedstuffs

Published Online: Apr 28, 2017

Page range: 491 - 502

Received: Jan 20, 2016

Accepted: Sep 26, 2016

DOI: https://doi.org/10.1515/aoas-2016-0059

Keywords
biohydrogenation, gas production, goat, methanogenesis, rumen, tannic acid

© by Yong M. Goh

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

An in vitro study on the ability of tannic acid to inhibit methanogenesis and biohydrogenation of C18 PUFA in the rumen of goats

Article Category: Animal nutrition, and feedstuffs

Published Online: Apr 28, 2017

Page range: 491 - 502

Received: Jan 20, 2016

Accepted: Sep 26, 2016

DOI: https://doi.org/10.1515/aoas-2016-0059

Keywordsbiohydrogenation, gas production, goat, methanogenesis, rumen, tannic acid

© by Yong M. Goh

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
biohydrogenation, gas production, goat, methanogenesis, rumen, tannic acid