Host Status of Cover Crops for the Management of Pratylenchus Jaehni on Coffee

Abstract The lesion nematode Pratylenchus jaehni occurs at low frequency in Brazilian coffee orchards but could provoke extensive root damage. Intercropping cover crops is a traditional practice in Brazilian coffee orchards, and the use of non-hosts of P. jaehni as cover crops may be a useful management method. In this work, 10 cover crops were tested concerning reproduction of P. jaehni. Cajanus cajan, Canavalia ensiformis, and Mucuna deeringiana are cover crops commonly used as intercropping in coffee orchards, but they must not be used in orchards infested with P. jaehni, because they are good hosts of this nematode. Brachiaria ruziziensis, Crotalaria juncea, Dolichos lablab, and Pennisetum glaucum were considered poor hosts. Helianthus annuus cv. Catissol and cv. Uruguai and Crotalaria spectabilis proved to be non-hosts to P. jaehni, and therefore, they are the cover crops recommended in coffee orchards infested with this nematode.

Intercropping cover crops in orchards of arabica and canephora coffee (Coffea arabica and C. canephora, respectively) is a traditional practice in many countries, due to the benefits of cover cropping on weed management, soil conservation, and reduction of chemical use in coffee farming (Opoku-Ameyaw et al., 2003;Santos et al., 2016). In Brazil, nitrogenfixing plants, such as the mucunas (Mucuna spp.), pigeon pea (Cajanus cajan), jack bean (Canavalia ensiformis), and the crotalarias (Crotalaria spp.), are frequently used as cover crops for coffee because they improve the soil fertility by supplying organic nitrogen (Paulo et al., 2001;Bergo et al., 2006;Partelli et al., 2011). Furthermore, cover crops that are nonhost to plant-parasitic nematodes may be used for the management of coffee-parasitic nematodes. For example, planting showy rattlebox (Crotalaria spectabilis) as cover crop between rows had been found to manage root-knot nematodes on coffee in Brazil (Jaehn, 1984).
In Brazil, the most prevalent nematodes in coffee plantations are Meloidogyne exigua, Meloidogyne incognita, and Meloidogyne paranaensis (Villain et al., 2018). Therefore, most of the efforts in nematode management in Brazil are dedicated to suppression of the root-knot nematodes. A survey of nematode infestation in coffee orchards of São Paulo state showed that Pratylenchus brachyurus occurred in 18.3% from 235 samples, Pratylenchus jaehni in 5.1%, and Pratylenchus vulnus in 0.4% . In contrast to the situation prevailing with root-knot nematodes, the management of lesion nematodes in Brazilian coffee orchards is often being ignored.
In pot experiments, both C1 and C2 reproduced on Rangpur lime (Citrus limonia) but not on arabica coffee (Wilcken et al., 2008;Bonfim et al., 2011;Oliveira et al., 2011), while the population K5 reproduces on coffee (Silva and Inomoto, 2002).  reported that the population K5 is as aggressive as M. incognita to the arabica coffee plants. Considering the extensive damage caused by population K5 of P. jaehni in the roots of arabica coffee and some cultivars of canephora coffee (Tomazini et al., 2005;, and the absence of nematicides (biological and synthetic) for P. jaehni, there is a need to find a cover crop that is effective in managing P. jaehni. The objective of this project is to evaluate the host status of 10 tropical cover crops commonly used in Brazil to suppress P. jaehni.

Seedling preparation and nematode inoculum
The substrate used in experiments was sandy clay loam soil (75.2% sand, 3.4% silt, 21.4% clay, 1.3% organic matter, pH 6.3) treated with steam heat (121°C for 2 hr). Seeds of the cover crops were sowed directly in the pots (12-cm tall and 7.3-cm diam. plastic pots containing 480 cm 3 of soil). At 7 d after germination, the seedlings were thinned to three plants per pot in Experiment 1, and two plants per pot in Experiment 2, as these numbers were found adequate, considering the size of the pots.
Nematode inoculum of P. jaehni was the same as that used in the studies of Silva and Inomoto (2002), and Oliveira et al. (2011). They were obtained from roots of arabica coffee collected in the municipality of Marília, São Paulo state, in 1998. According to Oliveira et al. (2011), this isolate showed high homology level on D2-D3 expansion fragments (99% and 98%, respectively) with an isolate of P. jaehni deposited in the GenBank. The nematode was maintained in the laboratory on alfalfa callus (Riedel et al., 1973), and in glasshouse on roots of grain sorghum cv. Sara. Inoculum of P. jaehni was extracted from sorghum roots by a modified Baermann method (Hooper, 1986), and prepared to Pi of 200 juveniles and adults per pot. The P. jaheni inoculum (2 mL) was distributed into two holes per pot made in the soil, close to either side of the seedlings. After the nematode inoculation, the seedlings were maintained in a shaded place for 2 d to avoid heat stress on the nematode and then were transferred to a glasshouse, where they were maintained until evaluation.

Experimental design
Both experiments were established in a completely randomized design with 11 treatments and six replicates (Experiment 1), and seven treatments and six replicates (Experiment 2).

Evaluation
Final density of P. jaehni on the cover crops was assessed 75 d after inoculation in Experiment 1, and 90 d after inoculation in Experiment 2. Testing periods were based on the cycle of pearl millet, which is the tested plant with a shorter life cycle (100 d approximately). Pots were immersed in a bucket containing 4 L of tap water in order to separate the roots from the soil. Roots were washed with tap water, dried on absorbent paper, cut in 1-cm pieces, and weighted. A subsample of 10 g of each replicate was processed by blender followed by centrifugal-flotation (Coolen and D'Herde, 1972), using a centrifuge with four 126-mL tubes (10-cm high and 4-cm diam.) at 580 g. Pf was estimated by counting juveniles and adults recovered from the roots. The variables used for comparison were the Rf, i.e., the ratio between the Pf and the Pi, and nematode per gram of fresh root.

Statistical analysis
All data collected were subjected to one-way analysis using SAS statistical software (SAS Institute, 2003). Means were separated by Tukey's Honestly Significant Difference Test (P = 0.05) wherever appropriate. The Rf of the cover crops was compared with the Rf of sorghum, which is a good host of P. jaehni (Silva and Inomoto, 2002). Plants with Rf > 1 and not differing statistically from sorghum were considered good hosts of P. jaehni; plants with 1 < Rf < Rf of sorghum were considered poor hosts; and plants with Rf < 1 were considered non-hosts.

Results and Discussion
In Experiment 1, jack bean, dwarf mucuna, and dwarf pigeon pea cv. Iapar-43 were rated as good hosts of P. jaehni; hyacinth bean and Kennedy ruzi grass were poor hosts; and sunflower cv. Uruguai, sunflower cv. Catissol, sunn hemp cv. IAC-KR-1, pearl millet cv. ADR-300, and showy rattlebox were non-hosts (Table 1). Results from Experiment 2 were similar to Experiment 1, where Kennedy ruzi grass was a poor host, while sunflower cv. Uruguai, sunflower cv. Catissol, and showy rattlebox were non-hosts. Although sunn hemp cv. IAC-KR-1 and pearl millet cv. ADR-300 were rated as non-hosts in Experiment 1, they allowed a low reproduction rate of P. jaehni, and therefore should be considered as poor hosts.
Intercropping of jack bean, dwarf mucuna, hyacinth bean, and pearl millet was suggested to provide weed management in coffee orchards (Partelli et al., 2010;Martins et al., 2015;Santos et al., 2016). Jack bean, dwarf mucuna, and pigeon pea are leguminous cover crops that can fix nitrogen, and upon termination of these cover crops, their residues may supply part of the nitrogen needs for coffee (Partelli et al., 2011). Legumes such as showy rattlebox, pigeon pea, and hyacinth bean had been demonstrated to increase coffee productivity when intercropping as ground cover with coffee (Guimarães et al., 2016). However, cover crops planted too close to the coffee plants may provoke coffee yield reduction, due to competition for water, light, and nutrients (Paulo et al., 2001). The current study showed that another detrimental effect of cover crops may be the increase of population density of P. jaehni.
The current study demonstrated that jack bean, dwarf mucuna, and pigeon pea were good hosts of P. jaehni (population K5). Therefore, they should not be used as cover crops in coffee orchards infested with this nematode. This is the first report of hyacinth bean and Kennedy ruzi grass as poor hosts of P. jaehni (population K5), as they only allow a smaller increase in population density of the nematode than grain sorghum. Sunn hemp and pearl millet were nonhosts of P. jaehni (Rf < 1) in Experiment 1 but were poor hosts (Rf > 1) in Experiment 2. This result was different from a previous study where an unknown cultivar of Table 1. Reproduction factors (Rf) of Pratylenchus jaehni and nematodes per fresh root mass (Nem/g) of cover crops at 75 d (Experiment 1) and 90 d after nematode inoculation (Experiment 2).  (Silva and Inomoto, 2002). Inconsistency of Rf values for sunn hemp and pearl millet on P. jaehni suggested that these cover crops should not be recommended for intercropping with coffee in a P. jaehni infested field. When the host suitability of the same cover crops was assessed for P. brachyurus, both Kennedy ruzi grass and pearl millet cv. ADR-300 were considered poor hosts (Inomoto and Asmus, 2010), whereas sunn hemp (Machado et al., 2007) and hyacinth bean (unpubl. data) were rated as good hosts. Sunflower is another cover crop used in coffee orchards in Brazil (Wutke et al., 2014;Espindula et al., 2015). Including the current study, three sunflower cultivars (cv. Uruguai, cv. Catissol, and cv. Morgan-734) are now confirmed to decrease the population density of P. jaehni (Silva and Inomoto, 2002). However, intercropping is recommended only in the first 3 yr of coffee crop to avoid competition with coffee plants (Espindula et al., 2015). Notwithstanding, Asmus et al. (2005) reported that sunflower cv. Uruguai was a good host to M. incognita. Similarly, Dias-Arieira et al. (2008) found that eight sunflower cultivars, including cv. Catissol, were susceptible to M. incognita. Conversely, the same authors found four resistant cultivars of sunflower to M. paranaensis, another important plant-parasitic nematode of coffee plants. As for P. brachyurus, Dias et al. (2016) identified several sunflower genotypes resistant to this species but not to M. incognita.
Showy rattlebox was never tested for its host status on P. jaehni. This cover crop has been largely used in double-crop with soybean to manage P. brachyurus, mainly in the Central-West region of Brazil, with an estimated 4 million ha sowed in 2018 (Souza and Inomoto, 2019). It is considered as a non-host to P. brachyurus (Machado et al., 2007). Previous reports also demonstrated its resistance to M. incognita (Linde, 1956), and M. exigua (Almeida and Campos, 1991). The suitability of this cover crop for M. paranaensis is unknown.
In conclusion, showy rattlebox and sunflower may be used as cover crops for intercropping with coffee in orchards infested by the population K5 of P. jaehni due to their non-host status to this plantparasitic nematode on coffee. Whereas hyacinth bean and Kennedy ruzi grass are rated as poor hosts of P. jaehni, jack bean, dwarf mucuna, and pigeon pea should not be intercropped with coffee in a population K5 P. jaehni infested field due to their high susceptibility to this population of nematode.