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Volume 69 (2022): Edizione 2 (August 2022)

Volume 69 (2022): Edizione s1 (July 2022)

Volume 69 (2022): Edizione 1 (January 2022)

Volume 68 (2021): Edizione 2 (December 2021)

Volume 68 (2021): Edizione 1 (January 2021)

Volume 67 (2020): Edizione 2 (May 2020)

Volume 67 (2020): Edizione 1 (January 2020)

Volume 66 (2019): Edizione 2 (November 2019)

Volume 66 (2019): Edizione 1 (June 2019)

Volume 65 (2018): Edizione 2 (November 2018)

Volume 65 (2018): Edizione 1 (June 2018)

Volume 64 (2017): Edizione 2 (November 2017)

Volume 64 (2017): Edizione 1 (September 2017)

Volume 63 (2016): Edizione s12 (December 2016)

Volume 63 (2016): Edizione 2 (December 2016)

Volume 63 (2016): Edizione 1 (September 2016)

Volume 62 (2015): Edizione s10 (September 2015)

Volume 62 (2015): Edizione s9 (June 2015)

Volume 62 (2015): Edizione 2 (December 2015)

Volume 62 (2015): Edizione 1 (June 2015)

Volume 61 (2014): Edizione 2 (December 2014)

Volume 61 (2014): Edizione 1 (August 2014)

Volume 60 (2013): Edizione Supplementum-VIII (March 2013)
Rare disease day in Slovakia, Europlan national conference

Volume 60 (2013): Edizione 2 (December 2013)

Volume 60 (2013): Edizione 1 (June 2013)

Volume 59 (2012): Edizione Supplement-VII (February 2012)
Conference abstracts, Rare disease day, conference 29.2.2012, First Slovak conference about rare diseases

Volume 59 (2012): Edizione Supplement-VI (January 2012)
Clinical Pharmacy in the Slovak Republic, dedicated to the associated professor Lívia Magulová, PhD.

Volume 59 (2012): Edizione 2 (November 2012)

Volume 59 (2012): Edizione 1 (June 2012)

Volume 58 (2011): Edizione 1 (January 2011)

Dettagli della rivista
Formato
Rivista
eISSN
2453-6725
Pubblicato per la prima volta
25 Nov 2011
Periodo di pubblicazione
2 volte all'anno
Lingue
Inglese

Cerca

Volume 67 (2020): Edizione 2 (May 2020)

Dettagli della rivista
Formato
Rivista
eISSN
2453-6725
Pubblicato per la prima volta
25 Nov 2011
Periodo di pubblicazione
2 volte all'anno
Lingue
Inglese

Cerca

6 Articoli

Original Paper

access type Accesso libero

The Antioxidant Analysis of Selected Types of Climbing Plants with Therapeutic Effect

Pubblicato online: 29 Jan 2021
Pagine: 1 - 4

Astratto

Abstract

Climbing plants with therapeutic effects are relatively little studied group of medicinal species. In the collection of climbing species planted in 2019 in the Botanical Garden of the Slovak University of Agriculture (SUA) in Nitra, we observed the growth and potential food, horticulture and healing use. For the antioxidant analysis, 7 species from 152 plants of 13 genera and 22 species were selected. The highest antiradical activity was recorded in the species Vigna sinensis, where reached average measured values achieved up to 73.79%. However, the related species Vigna unguiculata achieved only 26% of antioxidant activity. Of the fruits, the highest activity was Momordica charantia in immature state (73%). Other fruits showed very low antiradical activity

Parole chiave

  • Antioxidant activity
  • climbing plants
  • fruits
  • medicinal species
  • therapeutic effect
access type Accesso libero

Functionality evaluation of co-processed excipients as diluents in tablets manufactured by wet granulation

Pubblicato online: 29 Jan 2021
Pagine: 5 - 14

Astratto

Abstract

Diluents are essential components of a tablet formulation. The type of diluent used in a formulation influences the quality of tablets produced from that formulation. The aim of this study was to evaluate the tableting properties of co-processed excipients (C-PEs) incorporated as diluents in tablet formulation by wet granulation. Metronidazole tablets were prepared by wet granulation incorporating different diluents that were either single component excipients (SCEs) (lactose and microcrystalline cellulose) or C-PEs (Ludipress®, StarLac®, Prosolv® and AVICEL®HFE). The granules obtained for each formulation were evaluated for particle size analysis, flow properties and compression properties. Tablets weighing 500 mg were compressed from the metronidazole granules on a Single Station Tablet Press using a 12 mm punch and die tooling system. The tablets were kept for 24 h post-production, and the properties of weight uniformity, thickness, tensile strength, friability, disintegration time and dissolution profile evaluated subsequently. Results of granule properties showed that variations in parameters evaluated was as a result of differences in the type and composition of diluent used in formulation. Compactibility and tabletability profile of metronidazole granules revealed a better performance with granules processed with C-PE based diluents compared to SCE-based diluents. Tablets formulated with C-PEs as diluents were uniform in tablet weight, disintegrated faster and yielded a faster drug release compared to tablet formulations containing SCEs as diluent. This study reveals the performance advantage of C-PEs as diluents in tablets manufactured by wet granulation and highlights the importance of rational selection of excipients during tablet formulation.

Parole chiave

  • Diluents
  • co-processed excipients
  • tablets
  • wet granulation
access type Accesso libero

Preparation and evaluation of an oral mucoadhesive gel containing nystatin-loaded alginate microparticles

Pubblicato online: 11 Mar 2021
Pagine: 15 - 21

Astratto

Abstract

Nystatin is an antifungal agent used for prophylaxis and treatment of candidiasis, especially oral mycosis. Efficacy of nystatin conventional dosage forms is limited by the short residence time and bitter taste of the drug. This research aims at designing an optimized formulation of oral mucoadhesive gel of nystatin-loaded alginate microparticles, which can be retained in the mouth. Sodium alginate solution containing nystatin was added to the solution of calcium chloride under stirring. Microparticles containing nystatin were incorporated into the Carbopol gel. Size, loading, and release profile and mucoadhesion were investigated. The most suitable microparticles with particle size of < 250 μm were prepared with alginate concentration of 1%(w/v), calcium chloride of 1%(w/v), drug:polymer concentration 1%, and ratio of alginate solution:calcium chloride of 1:10. This formulation showed 49.1% drug loading and 98.2% encapsulation efficiency. Carbopol 934 gel provided optimal mucoadhesive properties. Release profile proved a burst release, which can be attributed to the surface associated drug, followed by a slower sustained release phase for all microparticles. The developed system with ability to adhere to the oral mucosa has great appeal for treatment of localized infections and can mask bitter taste of the drug and be retained in the mouth for long periods.

Parole chiave

  • Alginate
  • Carbopol
  • Microparticle
  • Mucoadhesion
  • Nystatin
access type Accesso libero

Toxicity of primulic acid 1 against a daphnid species Simocephalus expinosus s.l.

Pubblicato online: 03 Mar 2021
Pagine: 22 - 26

Astratto

Abstract

Primulic acid 1 is the main saponin present in Primula elatior. The present study describes the isolation of this amphiphilic compound from primula root. It was performed by ultrasonic maceration, reverse-phase column chromatography and crystallization. Investigations of its physicochemical property are represented by the determination of critical micelle concentration (cmc). The cmc value of the amphiphile was 9.4 × 10−5 mol·dm−3. The evaluation of environmental toxicity was performed on a daphnid species Simpocephalus expinosus s.l., which was very sensitive to primulic acids 1. The results from acute immobilisation test show that the tested compound has half maximal effective concentration after 24 hours (EC50-24 h) equal to 6.9 mg·l−1. Saponin can be classified as a toxic compound for aquatic organisms.

Parole chiave

  • Critical micelle concentration
  • daphnid
  • ecotoxicology
  • extraction
  • primula root
access type Accesso libero

Study of the Qualitative Composition and Quantitative Content of Parthenolide in the Feverfew (Tanacetum Parthenium) Herb Cultivated in Ukraine

Pubblicato online: 29 Jan 2021
Pagine: 27 - 33

Astratto

Abstract

Feverfew is a well-known medicinal plant as a source of the sesquiterpene lactones, the main one is a parthenolide (about 85%). Owing to the parthenolide, feverfew herb has anti-inflammatory activity and it is widely used for prevention of migraine. The aim was to study the qualitative composition and quantitative content of the parthenolide in the samples of the feverfew herb cultivated in Ukraine and to study the influence of environmental factors on the accumulation of parthenolide.

Methods

The identification in the samples of the feverfew herb by the thin-layer chromatography method was carried out on silica gel 60 F254 plates, Merck. For the quantitative determination of the parthenolide, a high-performance liquid chromatography ProStar equipped with a diode-matrix detector, were used.

Results

The chromatographic profile of the samples of the feverfew herb was studied using a TLC method. In the chromatograms of test solutions from all samples, the blue zones were detected at the level of parthenolide. The quantitative content of parthenolide ranged between 0.16% and 0.39%. The highest content was observed in the sample from the Central region of Ukraine. The data obtained indicate that the optimal conditions for parthenolide accumulation in feverfew herb are as follows: black soils, solar insolation not higher than 5.25 kWh/m2/day, average air temperature from +20°C to +25°C. All the calculated validation parameters of the parthenolide quantitative determination method meet the necessary acceptance criteria.

Conclusion

The study of the qualitative composition and quantitative content of parthenolide in the samples of feverfew herb cultivated in different regions of Ukraine has been conducted. According to the methodology of Eur.Ph. and SPhU, a parthenolide was identified in all the samples. HPLC method was used to determine the quantitative content of parthenolide in the samples studied. The optimal conditions for parthenolide accumulation in feverfew herb were established.

Parole chiave

  • Feverfew
  • parthenolide
  • thin-layer chromatography
  • high-performance liquid chromatography
  • environmental conditions
access type Accesso libero

Synthesis, antimicrobial and antiradical activity of (3-alkoxymethyl-4-hydroxyphenyl)propan-1-ones, intermediates of biologically active compounds and activity comparison with 3-(alkoxymethyl)-4-(alkylamino-2-hydroxypropoxyphenyl)alkanones type of beta blockers

Pubblicato online: 11 Mar 2021
Pagine: 34 - 44

Astratto

Abstract

A homologous series of (3-alkoxymethyl-4-hydroxyphenyl)propan-1-ones was prepared by the reaction of (3-chloromethyl-4-hydroxyphenyl)propan-1-ones with the corresponding alcohols (methanol – decan-1-ol, propan-2-ol, 2-methylpropan-1-ol, 3-methylbutan-1-ol, cyclopentanol, benzylalcohol) in the presence of sodium hydrogen carbonate. The composition of the synthesised compounds was elucidated by IR, UV and 1H-NMR and 13C-NMR spectra. Selected compounds were tested against human pathogens: gram-positive bacterium Staphylococcus aureus (CNCTC Mau 29/58), gram-negative bacterium Escherichia coli (CNCTC 377/79) and yeast Candida albicans (CCM 8186). Their antimicrobial activities were expressed as minimum inhibitory concentrations. Antioxidant activity was determined using DPPH and ABTS.+ methods. It could be shown that both biological activities, antimicrobial and antioxidant, were lower in comparison with the (2RS)-bis [3-(4-acetyl-2-propoxymethyl)phenoxy-2-hydroxypropyl]isopropylammonium fumarate type of beta blockers.

Parole chiave

  • substituted phenols
  • antimicrobial activity
  • antioxidant activity
  • antiradical activity
6 Articoli

Original Paper

access type Accesso libero

The Antioxidant Analysis of Selected Types of Climbing Plants with Therapeutic Effect

Pubblicato online: 29 Jan 2021
Pagine: 1 - 4

Astratto

Abstract

Climbing plants with therapeutic effects are relatively little studied group of medicinal species. In the collection of climbing species planted in 2019 in the Botanical Garden of the Slovak University of Agriculture (SUA) in Nitra, we observed the growth and potential food, horticulture and healing use. For the antioxidant analysis, 7 species from 152 plants of 13 genera and 22 species were selected. The highest antiradical activity was recorded in the species Vigna sinensis, where reached average measured values achieved up to 73.79%. However, the related species Vigna unguiculata achieved only 26% of antioxidant activity. Of the fruits, the highest activity was Momordica charantia in immature state (73%). Other fruits showed very low antiradical activity

Parole chiave

  • Antioxidant activity
  • climbing plants
  • fruits
  • medicinal species
  • therapeutic effect
access type Accesso libero

Functionality evaluation of co-processed excipients as diluents in tablets manufactured by wet granulation

Pubblicato online: 29 Jan 2021
Pagine: 5 - 14

Astratto

Abstract

Diluents are essential components of a tablet formulation. The type of diluent used in a formulation influences the quality of tablets produced from that formulation. The aim of this study was to evaluate the tableting properties of co-processed excipients (C-PEs) incorporated as diluents in tablet formulation by wet granulation. Metronidazole tablets were prepared by wet granulation incorporating different diluents that were either single component excipients (SCEs) (lactose and microcrystalline cellulose) or C-PEs (Ludipress®, StarLac®, Prosolv® and AVICEL®HFE). The granules obtained for each formulation were evaluated for particle size analysis, flow properties and compression properties. Tablets weighing 500 mg were compressed from the metronidazole granules on a Single Station Tablet Press using a 12 mm punch and die tooling system. The tablets were kept for 24 h post-production, and the properties of weight uniformity, thickness, tensile strength, friability, disintegration time and dissolution profile evaluated subsequently. Results of granule properties showed that variations in parameters evaluated was as a result of differences in the type and composition of diluent used in formulation. Compactibility and tabletability profile of metronidazole granules revealed a better performance with granules processed with C-PE based diluents compared to SCE-based diluents. Tablets formulated with C-PEs as diluents were uniform in tablet weight, disintegrated faster and yielded a faster drug release compared to tablet formulations containing SCEs as diluent. This study reveals the performance advantage of C-PEs as diluents in tablets manufactured by wet granulation and highlights the importance of rational selection of excipients during tablet formulation.

Parole chiave

  • Diluents
  • co-processed excipients
  • tablets
  • wet granulation
access type Accesso libero

Preparation and evaluation of an oral mucoadhesive gel containing nystatin-loaded alginate microparticles

Pubblicato online: 11 Mar 2021
Pagine: 15 - 21

Astratto

Abstract

Nystatin is an antifungal agent used for prophylaxis and treatment of candidiasis, especially oral mycosis. Efficacy of nystatin conventional dosage forms is limited by the short residence time and bitter taste of the drug. This research aims at designing an optimized formulation of oral mucoadhesive gel of nystatin-loaded alginate microparticles, which can be retained in the mouth. Sodium alginate solution containing nystatin was added to the solution of calcium chloride under stirring. Microparticles containing nystatin were incorporated into the Carbopol gel. Size, loading, and release profile and mucoadhesion were investigated. The most suitable microparticles with particle size of < 250 μm were prepared with alginate concentration of 1%(w/v), calcium chloride of 1%(w/v), drug:polymer concentration 1%, and ratio of alginate solution:calcium chloride of 1:10. This formulation showed 49.1% drug loading and 98.2% encapsulation efficiency. Carbopol 934 gel provided optimal mucoadhesive properties. Release profile proved a burst release, which can be attributed to the surface associated drug, followed by a slower sustained release phase for all microparticles. The developed system with ability to adhere to the oral mucosa has great appeal for treatment of localized infections and can mask bitter taste of the drug and be retained in the mouth for long periods.

Parole chiave

  • Alginate
  • Carbopol
  • Microparticle
  • Mucoadhesion
  • Nystatin
access type Accesso libero

Toxicity of primulic acid 1 against a daphnid species Simocephalus expinosus s.l.

Pubblicato online: 03 Mar 2021
Pagine: 22 - 26

Astratto

Abstract

Primulic acid 1 is the main saponin present in Primula elatior. The present study describes the isolation of this amphiphilic compound from primula root. It was performed by ultrasonic maceration, reverse-phase column chromatography and crystallization. Investigations of its physicochemical property are represented by the determination of critical micelle concentration (cmc). The cmc value of the amphiphile was 9.4 × 10−5 mol·dm−3. The evaluation of environmental toxicity was performed on a daphnid species Simpocephalus expinosus s.l., which was very sensitive to primulic acids 1. The results from acute immobilisation test show that the tested compound has half maximal effective concentration after 24 hours (EC50-24 h) equal to 6.9 mg·l−1. Saponin can be classified as a toxic compound for aquatic organisms.

Parole chiave

  • Critical micelle concentration
  • daphnid
  • ecotoxicology
  • extraction
  • primula root
access type Accesso libero

Study of the Qualitative Composition and Quantitative Content of Parthenolide in the Feverfew (Tanacetum Parthenium) Herb Cultivated in Ukraine

Pubblicato online: 29 Jan 2021
Pagine: 27 - 33

Astratto

Abstract

Feverfew is a well-known medicinal plant as a source of the sesquiterpene lactones, the main one is a parthenolide (about 85%). Owing to the parthenolide, feverfew herb has anti-inflammatory activity and it is widely used for prevention of migraine. The aim was to study the qualitative composition and quantitative content of the parthenolide in the samples of the feverfew herb cultivated in Ukraine and to study the influence of environmental factors on the accumulation of parthenolide.

Methods

The identification in the samples of the feverfew herb by the thin-layer chromatography method was carried out on silica gel 60 F254 plates, Merck. For the quantitative determination of the parthenolide, a high-performance liquid chromatography ProStar equipped with a diode-matrix detector, were used.

Results

The chromatographic profile of the samples of the feverfew herb was studied using a TLC method. In the chromatograms of test solutions from all samples, the blue zones were detected at the level of parthenolide. The quantitative content of parthenolide ranged between 0.16% and 0.39%. The highest content was observed in the sample from the Central region of Ukraine. The data obtained indicate that the optimal conditions for parthenolide accumulation in feverfew herb are as follows: black soils, solar insolation not higher than 5.25 kWh/m2/day, average air temperature from +20°C to +25°C. All the calculated validation parameters of the parthenolide quantitative determination method meet the necessary acceptance criteria.

Conclusion

The study of the qualitative composition and quantitative content of parthenolide in the samples of feverfew herb cultivated in different regions of Ukraine has been conducted. According to the methodology of Eur.Ph. and SPhU, a parthenolide was identified in all the samples. HPLC method was used to determine the quantitative content of parthenolide in the samples studied. The optimal conditions for parthenolide accumulation in feverfew herb were established.

Parole chiave

  • Feverfew
  • parthenolide
  • thin-layer chromatography
  • high-performance liquid chromatography
  • environmental conditions
access type Accesso libero

Synthesis, antimicrobial and antiradical activity of (3-alkoxymethyl-4-hydroxyphenyl)propan-1-ones, intermediates of biologically active compounds and activity comparison with 3-(alkoxymethyl)-4-(alkylamino-2-hydroxypropoxyphenyl)alkanones type of beta blockers

Pubblicato online: 11 Mar 2021
Pagine: 34 - 44

Astratto

Abstract

A homologous series of (3-alkoxymethyl-4-hydroxyphenyl)propan-1-ones was prepared by the reaction of (3-chloromethyl-4-hydroxyphenyl)propan-1-ones with the corresponding alcohols (methanol – decan-1-ol, propan-2-ol, 2-methylpropan-1-ol, 3-methylbutan-1-ol, cyclopentanol, benzylalcohol) in the presence of sodium hydrogen carbonate. The composition of the synthesised compounds was elucidated by IR, UV and 1H-NMR and 13C-NMR spectra. Selected compounds were tested against human pathogens: gram-positive bacterium Staphylococcus aureus (CNCTC Mau 29/58), gram-negative bacterium Escherichia coli (CNCTC 377/79) and yeast Candida albicans (CCM 8186). Their antimicrobial activities were expressed as minimum inhibitory concentrations. Antioxidant activity was determined using DPPH and ABTS.+ methods. It could be shown that both biological activities, antimicrobial and antioxidant, were lower in comparison with the (2RS)-bis [3-(4-acetyl-2-propoxymethyl)phenoxy-2-hydroxypropyl]isopropylammonium fumarate type of beta blockers.

Parole chiave

  • substituted phenols
  • antimicrobial activity
  • antioxidant activity
  • antiradical activity

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