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Effect of probiotics on immune cells in young Japanese Black calves responding to vaccination against bacterial respiratory diseases

Shogo Takeda

Shogo Takeda

  • Veterinary Medicine, Obihiro University of AgricultureObihiro-shi, Hokkaido, Japan
  • Hidaka C.L.CNiikappu-cho, Hokkaido, Japan
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Takeda, Shogo
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Hiromichi Ohtsuka

Hiromichi Ohtsuka

Veterinary Medicine, Obihiro University of AgricultureObihiro-shi, Hokkaido, Japan
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Ohtsuka, Hiromichi
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Keigo Kosenda

Keigo Kosenda

Veterinary Medicine, Rakuno Gakuen UniversityEbetsu-shi, Hokkaido, Japan
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Kosenda, Keigo
  
25 mar 2025
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Pubblicato online: 25 mar 2025
Pagine: 27 - 33
Ricevuto: 31 lug 2024
Accettato: 17 mar 2025
DOI: https://doi.org/10.2478/jvetres-2025-0013
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© 2025 Shogo Takeda et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Fig. 1.

Changes in antibody titres against Pasteurella multocida, Mannheimia haemolytica and Histophilus somni. Solid circles – research group; hollow circles – control group. Results are expressed as the mean ± standard error (n = 24). O.D. – optimal density
Changes in antibody titres against Pasteurella multocida, Mannheimia haemolytica and Histophilus somni. Solid circles – research group; hollow circles – control group. Results are expressed as the mean ± standard error (n = 24). O.D. – optimal density

Fig. 2.

Changes in numbers of peripheral lymphocyte, CD3+, CD4+, CD8+, CD14+ and MHC class-II cells. Solid circles – research group; hollow circles – control group. *–significant difference (P-value < 0.05) between two groups on the same day of life
Changes in numbers of peripheral lymphocyte, CD3+, CD4+, CD8+, CD14+ and MHC class-II cells. Solid circles – research group; hollow circles – control group. *–significant difference (P-value < 0.05) between two groups on the same day of life

Fig. 3.

Changes in relative gene expressions of interleukin (IL)-4, IL-12p40, IL-17A, interferon (IFN)-γ and transforming growth factor (TGF)-β. Solid circles – research group; hollow circles – control group. *–significant difference (P-value < 0.05) between two groups on the same day of life
Changes in relative gene expressions of interleukin (IL)-4, IL-12p40, IL-17A, interferon (IFN)-γ and transforming growth factor (TGF)-β. Solid circles – research group; hollow circles – control group. *–significant difference (P-value < 0.05) between two groups on the same day of life

Primers used for real-time PCR expression analysis

Gene GenBank Accession No. Product length (base pairs) Primer 5′–3′ sequence
IL-4 NM_173921 117 Forward GCCCCAAAGAACACAACTGA
Reverse GAGATTCCTGTCAAGTCCGC
IL-12p40 NM_174356.1 101 Forward CACCCCGCATTCCTACTTCT
Reverse TGACTTTGGCTGAGGTTTGG
IL-17A NM_001008412.2 138 Forward ATCTCACAGCGAGCACAAGT
Reverse GTGGGATGATGACTCCTGCC
IFN-γ NM_174086 108 Forward TCAAATTCCGGTGGATGATCT
Reverse CTTCTCTTCCGCTTTCTGAGG
TGF-β NM_001166068.1 140 Forward TGACCCGCAGAGAGGAAATAG
Reverse GTTCATGCCGTGAATGGTG
β-actin NM_173979.3 76 Forward TCTTCCATAGGACACAATGCC
Reverse GAGGCATACAGGGACAGCAC

Nutrient composition of diets in the Japanese Black calves

Dietary component Percentage of dry matter intake from commercial feed diet
Milk replacer Starter
TDN 116 75
CP 25 20
Fat 25 2
NDF 1 10
Lingua:
Inglese
Frequenza di pubblicazione:
4 volte all'anno
Argomenti della rivista:
Scienze biologiche, Biologia molecolare, Microbiologia e virologia, Scienze della vita, altro, Medicina, Medicina veterinaria
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