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Volume 68 (2022): Issue 2 (June 2022)

Volume 68 (2022): Issue 1 (March 2022)

Volume 67 (2021): Issue 4 (December 2021)

Volume 67 (2021): Issue 3 (September 2021)

Volume 67 (2021): Issue 2 (June 2021)

Volume 67 (2021): Issue 1 (March 2021)

Volume 66 (2020): Issue 4 (December 2020)

Volume 66 (2020): Issue 3 (September 2020)

Volume 66 (2020): Issue 2 (June 2020)

Volume 66 (2020): Issue 1 (March 2020)

Volume 65 (2019): Issue 4 (December 2019)

Volume 65 (2019): Issue 3 (September 2019)

Volume 65 (2019): Issue 2 (June 2019)

Volume 65 (2019): Issue 1 (March 2019)

Volume 64 (2018): Issue 4 (December 2018)

Volume 64 (2018): Issue 3 (September 2018)

Volume 64 (2018): Issue 2 (June 2018)

Volume 64 (2018): Issue 1 (March 2018)

Volume 63 (2017): Issue 4 (December 2017)

Volume 63 (2017): Issue 3 (September 2017)

Volume 63 (2017): Issue 2 (June 2017)

Volume 63 (2017): Issue 1 (March 2017)

Volume 62 (2016): Issue 4 (December 2016)

Volume 62 (2016): Issue 3 (September 2016)

Volume 62 (2016): Issue 2 (June 2016)

Volume 62 (2016): Issue 1 (March 2016)

Volume 61 (2015): Issue 4 (December 2015)

Volume 61 (2015): Issue 3 (September 2015)

Volume 61 (2015): Issue 2 (June 2015)

Volume 61 (2015): Issue 1 (March 2015)

Volume 60 (2014): Issue 4 (December 2014)

Volume 60 (2014): Issue 3 (September 2014)

Volume 60 (2014): Issue 2 (June 2014)

Volume 60 (2014): Issue 1 (March 2014)

Volume 59 (2013): Issue 4 (December 2013)

Volume 59 (2013): Issue 3 (September 2013)

Volume 59 (2013): Issue 2 (June 2013)

Volume 59 (2013): Issue 1 (March 2013)

Journal Details
Format
Journal
eISSN
2449-8343
First Published
04 Apr 2014
Publication timeframe
4 times per year
Languages
English

Search

Volume 67 (2021): Issue 4 (December 2021)

Journal Details
Format
Journal
eISSN
2449-8343
First Published
04 Apr 2014
Publication timeframe
4 times per year
Languages
English

Search

10 Articles
Open Access

In memoriam of Professor Waldemar Buchwald (1962–2020) on the first anniversary of his passing

Published Online: 02 Feb 2022
Page range: XIV - XIX

Abstract

Open Access

Tentative qualitative and quantitative analysis of phenolic compounds in leaf extract from Carica papaya Linn. plant growing in Uganda

Published Online: 02 Feb 2022
Page range: 1 - 9

Abstract

Summary Introduction

Carica papaya L. belongs to Caricaceae family. It is an evergreen tree or shrub with usually unbranched trunk. Previous studies on the methanolic leaf extract of this plant proved the antibacterial, cytotoxic, anticancer and antioxidant effects indicative of promising therapeutic potentials.

Methods

Chromatographic and spectroscopic techniques along with high performance liquid chromatography quantitative analysis were performed to isolate, tentatively identify and quantify polyphenolic compounds in MeOH extract of C. papaya leaves. UHPLC-DAD-ESI-MS/MS and UHPLC-DAD methods were adopted for qualitative and quantitative analysis respectively. In the case of polyphenol separation, some reference substances were isolated by preparative HPLC.

Results

Six polyphenols were isolated and quantified from C. papaya leaf extract, including three phenolic acids: derivatives of caffeic acid, p-coumaric acid, ferulic acid and three flavonoids: quercetin dirhamnosylhexoside, kaempferol dirhamnosyl-hexoside, quercetin 3-O-rutinoside. Among them quercetin dirhamnosyl-hexoside was significantly higher (1.9 mg/g extract) than other identified compounds.

Conclusion

The results of present study provide a new data on polyphenols composition of C. papaya plant derived from Uganda and promote research on its possible standardization and use as a dietary supplement and/or herbal medicine.

Keywords

  • Carica papaya L.
  • polyphenols
  • methanolic extract
  • UHPLC-DAD
  • UHPLC-DAD-MS
  • preparative-HPLC
Open Access

Cytotoxic roles of apigenin and kaempferol on staurosporine-treated mesenchymal stem cells in an in vitro culture

Published Online: 02 Feb 2022
Page range: 10 - 15

Abstract

Summary Introduction

Flavonoids are widely distributed in the wild. They constitute a large group of compounds that have a beneficial effect on the human body. Apigenin and kaempferol, which belong to the flavone subgroup, have, inter alia, an antitumor effect. The influence of these compounds on the survival of stem cells in a toxic environment has not yet been studied.

Objective

The aim of the study was to evaluate the effect of selected concentrations of apigenin and kaempferol on the survival of mesenchymal stem cells (MSC) in the presence of a cell-death inducer – staurosporine.

Methods

Mesenchymal stem cells that were obtained from the Wharton’s jelly of umbilical cords were used for the research. In the first stage, the MSC were treated with apigenin at concentrations of 1.2, 12.5, 25, 50 and 100 µM/ml and kaempferol at concentrations of 1.2, 12.5, 25, 50 and 100 µM/ml. In the next stage, the effect of increased concentrations of 0.1, 0.5 and 1 µM/ml of staurosporine on MSC was examined. The key stage of the experiment was investigating the interaction between the selected concentrations of apigenin (12.5 and 50 µM/ml) and kaempferol (12.5 and µM/ml) on MSC in the presence of staurosporine at a concentration of 1 µM/ml, which had the highest toxicity.

Results

Both apigenin and kaempferol significantly increased the cytotoxic features of staurosporine on the MSC culture.

Keywords

  • apigenin
  • kaempferol
  • staurosporine
  • cytoprotection
  • cytotoxicity
  • mesenchymal stem cells
Open Access

HPLC-DAD phenolics screening and in vitro investigation of haemostatic, antidiabetic, antioxidant and photoprotective properties of Centaurea tougourensis Boiss. & Reut.

Published Online: 02 Feb 2022
Page range: 16 - 31

Abstract

Summary Introduction

Traditional medicine has an important place in human history and this since antiquity. Indeed, during Egyptian and Chinese civilization era, many detailed manuscripts, describing the therapeutic effect of plants, were found which suggest that folk medicine is the basis of the actual medicine.

Objective

To investigate the phytochemical and pharmacological properties of the n-butanol (n-BuOH) and ethyl acetate (EA) extracts of the aerial part of Centaurea tougourensis.

Methods

The phytochemical evaluation was done based on HPLC-DAD approach. The antioxidant activity was determined by DPPH and cupric ion reducing antioxidant capacity (CUPRAC), while the hemostatic effect was performed using plasma recalcification time (PRT) method. The antidiabetic capacity was investigated by alpha-amylase inhibition assay and the photoprotective test was evaluated by the measurement of sun protection factor (SPF).

Results

13 phenolic compounds were identified in both extracts of C. tougourensis. These extracts showed antioxidant, haemostatic, antidiabetic and photoprotective properties with a dose-dependent manner. Amounts of n-BuOH activities were found higher, with a respective IC50 value of 0.72±0.07 μg/ml in DPPH assay, an A0.50 value lower than 3.125 μg/ml in CUPRAC assay besides a shortening rate percentage of coagulation (86.71%) in haemostatic assay, a moderate inhibition effect on alpha amylase activity with an IC50 value of (711.5±0.03 μg/ml) and a maximum sun protection factor of (56.035). These results were mostly found highly significant (p<0.001) when compared to respective standards.

Conclusion

This study demonstrated some pharmacological effects of C. tougourensis which suggests that our plant could be a good candidate to treat some illnesses related to oxidative stress, bleeding or skin cancer.

Keywords

  • Centaurea tougourensis
  • HPLC-DAD
  • phenolics
  • pharmacological properties
Open Access

Phytochemical screening and galactagogic attributes of Pergularia daemia (Forssk.) Chiov. leaf extract in lactating mice (Mus musculus)

Published Online: 02 Feb 2022
Page range: 32 - 41

Abstract

Summary Introduction

Pergularia daemia (Forssk.) Chiov. [PD] is used as a galactagogue by various tribes in Purulia district, India, lacks a scientific basis.

Objectives

The purpose of the study was to determine whether administration of aqueous leaf extract of P. daemia can increase milk production in lactating mice and encourages development in suckling pups.

Methods

30 lactating mice (n=6 per group) with a minimum of 5 pups each were treated with the aqueous PD extract at 50, 100, 150 and 200 mg/kg body weight while the control group was administered with only distilled water. The mice were daily fed with aqueous leaf extract via gavage, which started at day 3 and continued to day 15 post-partum and milk secretion was assessed by the weight-suckle-weight method. The qualitative estimation, UV-vis analysis and GC-MS study of the leaf extract was also done for the presence of phytoconstituents.

Results

Administration of aqueous leaf extract led to a considerable increase in milk production (28.1%) and the pups gained weight substantially (p<0.05) when compared to control. Milk production was enhanced in mice treated with 150 mg/kg b.wt. of leaf extract compared to other doses (p<0.05). The GC-MS analysis of the aqueous leaf extract of P. daemia revealed 20 major peaks, some with small retention time and also revealing presence of several phyto-estrogenic compounds.

Conclusion

The present study effectively demonstrated that aqueous leaf extract of PD enhanced milk production in mice and might be used as an alternative strategy for increasing milk production and can be extrapolated to farm animals and human beings.

Keywords

  • GC-MS
  • hormones
  • lactation
  • phytochemicals
  • Pergularia daemia
Open Access

Preliminary studies of Volten VR4® Kaempferia parviflora herb extracts on blood glucose levels in human type-2 diabetes mellitus and its mineral element analysis

Published Online: 02 Feb 2022
Page range: 42 - 53

Abstract

Summary Introduction

Volten VR4® capsules containing herb extract of Kaempferia parviflora has been claimed to reduce blood glucose in patients with diabetes.

Objectives

This preliminary study is conducted to evaluate the efficacy of Volten VR4® on healthy individuals and type-2 diabetes mellitus volunteers. The extracts of 400 mg capsules of Kaempferia parviflora (KP) were used to measure the blood glucose level of 2-hour postprandial.

Methods

The healthy group consists of 15 young adults aged 20-30 with no history of serious diseases, while the diabetic group includes 12 individuals aged 35-75 diagnosed with type-2 diabetes mellitus. Data were validated through the Willcoxon and Friedman test statistics and error distribution. The investigation was continued to trace the capsules contents of elements using inductively coupled plasma optical emission spectrometry (ICP-OES) techniques.

Results

It has been shown that KP reducing blood sugar levels has been associated with flavonoids and methoxyflavones components. The result specifically showed that consuming VR4® capsules can significantly reduce blood glucose, either at the state of fasting or postprandially. In the study the content of mineral and heavy metal elements in VR4® capsules has been evaluated.

Conclusion

Volten VR4® Kaempferia parviflora extract is safe to be consumed at a single dose of 400 mg. The study also has shown that the participants are free from adverse reactions and hypoglycaemia.

Keywords

  • Kaempferia parviflora
  • blood glucose
  • type-2 diabetes mellitus
  • flavonoids
Open Access

Occurrence of pathogenic fungi on commercially available medicinal plants and packaged seeds

Published Online: 02 Feb 2022
Page range: 54 - 59

Abstract

Summary Introduction

Pathogenic fungi decrease the quality of commercially available medicinal plants and packaged seeds. Some of fungal pathogens can produce mycotoxins harmful to human health.

Objective

To evaluate the health status of commercially available medicinal plants and check if packaged seeds of these plants are free from pathogenic fungi.

Methods

An experiment was conducted on potted plants of lemon balm, peppermint and sweet basil, commercially available and intended for direct use by consumers. For microbiological analyses, 100 stems and leaves of each species were randomly taken. Furthermore, the health of 100 seeds of each species was examined using agar plate method.

Results

Ten species and genera of pathogenic fungi were isolated from shoots, leaves and seeds of investigated plants. Some of them are toxin-forming species. Lemon balm was the most infected species.

Conclusion

Bearing in mind the health of consumers, more attention should be paid to the microbiological purity of commercially available medicinal plants and packaged seeds.

Keywords

  • Lamiaceae
  • mycotoxins
  • Melissa officinalis
  • Mentha piperita
  • Ocimum basilicum
  • pathogenic fungi
Open Access

Kinetic study on peroxidase inactivation and anthocyanin degradation of black cherry tomatoes (Solanum lycopersicum cv. OG) during blanching

Published Online: 02 Feb 2022
Page range: 60 - 69

Abstract

Summary Introduction

Blanching is a necessary treatment stage in processing of tomato products to inactivate enzymes. However, it may cause the degradation of nutrients.

Objective

In this study, the kinetics of thermal peroxidase inactivation and anthocyanin degradation in black cherry tomatoes (cv. OG) were determined to predict the quality changes during the blanching.

Methods

Tomatoes were blanched at five levels of temperature (75–95oC) for five time periods (30–150 s).

Results

It was found that as the blanching temperature increased and the blanching time is prolonged, more peroxidase was inactivated and the greater number of anthocyanins was lost. The thermal peroxidase inactivation and anthocyanin degradation showed an apparent first-order reaction with the activation energy of 129.96 kJ/mol and 65.99 kJ/mol, respectively. Peroxidase and anthocyanin in black cherry tomatoes were found to be heat-sensitive.

Conclusion

These kinetic parameters were necessary to select and design appropriate blanching conditions for black cherry tomatoes on larger scale processing.

Keywords

  • anthocyanin
  • black cherry tomatoes
  • blanching
  • kinetic study
  • peroxidase
  • Solanum lycopersicum cv. OG
Open Access

Selection of duplicates of flax accessions – an important task in the management of collection of genetic resources of Linum usitatissimum L.

Published Online: 02 Feb 2022
Page range: 70 - 76

Abstract

Summary Introduction

The Institute of Natural Fibres and Medicinal Plants National Research Institute (IWNiRZ) – formerly Institute of Natural Fibres, together with Plant Breeding and Acclimatization Institute (IHAR) in Radzików carry out tasks related to the protection of genetic resources of flax (Linum usitatissimum L.). Genetic resources of flax are stored as seeds in IHAR refrigeration chambers. The research material consisted of passport data of genetic resources of flax.

Objective

The aim of the presenting the identification of duplicates of accessions of flax in documentation of the National Center of Plant Genetic Resources (IHAR). This will facilitate the management of the collection of flax genetic resources. Removal of duplicate accessions from the collection of flax genetic resources will save storage space in long-term chambers, savings in storage costs, savings in working hours.

Methods

The working methods were studying the passport database and the institute’s gene bank documentation.

Results

In the cooling chambers of the seed bank at KCRZG at IHAR, unintentional duplicates are stored: 29 flax accessions with the same accessions number and 19 flax accessions with two different accession numbers.

Conclusions

The presentation in the article of two types of duplicates of flax accessions is very helpful in managing the collection of genetic resources of flax. Reducing the number of genotypes in the flax collection will facilitate the faster collection of all valorization data, which will be very helpful for users of the collection of genetic resources of flax.

Keywords

  • genetic resources
  • flax
  • Linum usitatissimum L.
  • duplicates of accessions
  • collection management
Open Access

Probiotics in prevention and treatment of cardiovascular diseases

Published Online: 02 Feb 2022
Page range: 77 - 85

Abstract

Summary

Increasing knowledge of the gut microbiota and its interference in human homeostasis in recent years has contributed to a better understanding of number of different interactions occurring in the gastrointestinal tract. Disruption of the microbiota is detrimental to health and contributes to the development of numerous diseases and may also be an accelerator of pathophysiological processes such as atherosclerosis. Cardiovascular diseases are the most common cause of death worldwide, so the development of new methods to support the treatment and prevention of these diseases becoms one of the priorities of modern medicine. Probiotics may constitute an important element of support in the treatment and prevention of CVD (cardiovascular diseases). A number of papers support such a statement, however, larger clinical trials are needed. Through a number of mechanisms including mitigating inflammation, sealing the intestinal epithelium, and affecting metabolism, probiotics may have a beneficial effect on general health and slow down the pathogenesis of many diseases, including those affecting the cardiovascular system. This article contains a review of current discoveries on the role of probiotics in the prevention and support of CVD treatment.

Keywords

  • probiotics
  • gastrointestinal diseases
  • microbiota
10 Articles
Open Access

In memoriam of Professor Waldemar Buchwald (1962–2020) on the first anniversary of his passing

Published Online: 02 Feb 2022
Page range: XIV - XIX

Abstract

Open Access

Tentative qualitative and quantitative analysis of phenolic compounds in leaf extract from Carica papaya Linn. plant growing in Uganda

Published Online: 02 Feb 2022
Page range: 1 - 9

Abstract

Summary Introduction

Carica papaya L. belongs to Caricaceae family. It is an evergreen tree or shrub with usually unbranched trunk. Previous studies on the methanolic leaf extract of this plant proved the antibacterial, cytotoxic, anticancer and antioxidant effects indicative of promising therapeutic potentials.

Methods

Chromatographic and spectroscopic techniques along with high performance liquid chromatography quantitative analysis were performed to isolate, tentatively identify and quantify polyphenolic compounds in MeOH extract of C. papaya leaves. UHPLC-DAD-ESI-MS/MS and UHPLC-DAD methods were adopted for qualitative and quantitative analysis respectively. In the case of polyphenol separation, some reference substances were isolated by preparative HPLC.

Results

Six polyphenols were isolated and quantified from C. papaya leaf extract, including three phenolic acids: derivatives of caffeic acid, p-coumaric acid, ferulic acid and three flavonoids: quercetin dirhamnosylhexoside, kaempferol dirhamnosyl-hexoside, quercetin 3-O-rutinoside. Among them quercetin dirhamnosyl-hexoside was significantly higher (1.9 mg/g extract) than other identified compounds.

Conclusion

The results of present study provide a new data on polyphenols composition of C. papaya plant derived from Uganda and promote research on its possible standardization and use as a dietary supplement and/or herbal medicine.

Keywords

  • Carica papaya L.
  • polyphenols
  • methanolic extract
  • UHPLC-DAD
  • UHPLC-DAD-MS
  • preparative-HPLC
Open Access

Cytotoxic roles of apigenin and kaempferol on staurosporine-treated mesenchymal stem cells in an in vitro culture

Published Online: 02 Feb 2022
Page range: 10 - 15

Abstract

Summary Introduction

Flavonoids are widely distributed in the wild. They constitute a large group of compounds that have a beneficial effect on the human body. Apigenin and kaempferol, which belong to the flavone subgroup, have, inter alia, an antitumor effect. The influence of these compounds on the survival of stem cells in a toxic environment has not yet been studied.

Objective

The aim of the study was to evaluate the effect of selected concentrations of apigenin and kaempferol on the survival of mesenchymal stem cells (MSC) in the presence of a cell-death inducer – staurosporine.

Methods

Mesenchymal stem cells that were obtained from the Wharton’s jelly of umbilical cords were used for the research. In the first stage, the MSC were treated with apigenin at concentrations of 1.2, 12.5, 25, 50 and 100 µM/ml and kaempferol at concentrations of 1.2, 12.5, 25, 50 and 100 µM/ml. In the next stage, the effect of increased concentrations of 0.1, 0.5 and 1 µM/ml of staurosporine on MSC was examined. The key stage of the experiment was investigating the interaction between the selected concentrations of apigenin (12.5 and 50 µM/ml) and kaempferol (12.5 and µM/ml) on MSC in the presence of staurosporine at a concentration of 1 µM/ml, which had the highest toxicity.

Results

Both apigenin and kaempferol significantly increased the cytotoxic features of staurosporine on the MSC culture.

Keywords

  • apigenin
  • kaempferol
  • staurosporine
  • cytoprotection
  • cytotoxicity
  • mesenchymal stem cells
Open Access

HPLC-DAD phenolics screening and in vitro investigation of haemostatic, antidiabetic, antioxidant and photoprotective properties of Centaurea tougourensis Boiss. & Reut.

Published Online: 02 Feb 2022
Page range: 16 - 31

Abstract

Summary Introduction

Traditional medicine has an important place in human history and this since antiquity. Indeed, during Egyptian and Chinese civilization era, many detailed manuscripts, describing the therapeutic effect of plants, were found which suggest that folk medicine is the basis of the actual medicine.

Objective

To investigate the phytochemical and pharmacological properties of the n-butanol (n-BuOH) and ethyl acetate (EA) extracts of the aerial part of Centaurea tougourensis.

Methods

The phytochemical evaluation was done based on HPLC-DAD approach. The antioxidant activity was determined by DPPH and cupric ion reducing antioxidant capacity (CUPRAC), while the hemostatic effect was performed using plasma recalcification time (PRT) method. The antidiabetic capacity was investigated by alpha-amylase inhibition assay and the photoprotective test was evaluated by the measurement of sun protection factor (SPF).

Results

13 phenolic compounds were identified in both extracts of C. tougourensis. These extracts showed antioxidant, haemostatic, antidiabetic and photoprotective properties with a dose-dependent manner. Amounts of n-BuOH activities were found higher, with a respective IC50 value of 0.72±0.07 μg/ml in DPPH assay, an A0.50 value lower than 3.125 μg/ml in CUPRAC assay besides a shortening rate percentage of coagulation (86.71%) in haemostatic assay, a moderate inhibition effect on alpha amylase activity with an IC50 value of (711.5±0.03 μg/ml) and a maximum sun protection factor of (56.035). These results were mostly found highly significant (p<0.001) when compared to respective standards.

Conclusion

This study demonstrated some pharmacological effects of C. tougourensis which suggests that our plant could be a good candidate to treat some illnesses related to oxidative stress, bleeding or skin cancer.

Keywords

  • Centaurea tougourensis
  • HPLC-DAD
  • phenolics
  • pharmacological properties
Open Access

Phytochemical screening and galactagogic attributes of Pergularia daemia (Forssk.) Chiov. leaf extract in lactating mice (Mus musculus)

Published Online: 02 Feb 2022
Page range: 32 - 41

Abstract

Summary Introduction

Pergularia daemia (Forssk.) Chiov. [PD] is used as a galactagogue by various tribes in Purulia district, India, lacks a scientific basis.

Objectives

The purpose of the study was to determine whether administration of aqueous leaf extract of P. daemia can increase milk production in lactating mice and encourages development in suckling pups.

Methods

30 lactating mice (n=6 per group) with a minimum of 5 pups each were treated with the aqueous PD extract at 50, 100, 150 and 200 mg/kg body weight while the control group was administered with only distilled water. The mice were daily fed with aqueous leaf extract via gavage, which started at day 3 and continued to day 15 post-partum and milk secretion was assessed by the weight-suckle-weight method. The qualitative estimation, UV-vis analysis and GC-MS study of the leaf extract was also done for the presence of phytoconstituents.

Results

Administration of aqueous leaf extract led to a considerable increase in milk production (28.1%) and the pups gained weight substantially (p<0.05) when compared to control. Milk production was enhanced in mice treated with 150 mg/kg b.wt. of leaf extract compared to other doses (p<0.05). The GC-MS analysis of the aqueous leaf extract of P. daemia revealed 20 major peaks, some with small retention time and also revealing presence of several phyto-estrogenic compounds.

Conclusion

The present study effectively demonstrated that aqueous leaf extract of PD enhanced milk production in mice and might be used as an alternative strategy for increasing milk production and can be extrapolated to farm animals and human beings.

Keywords

  • GC-MS
  • hormones
  • lactation
  • phytochemicals
  • Pergularia daemia
Open Access

Preliminary studies of Volten VR4® Kaempferia parviflora herb extracts on blood glucose levels in human type-2 diabetes mellitus and its mineral element analysis

Published Online: 02 Feb 2022
Page range: 42 - 53

Abstract

Summary Introduction

Volten VR4® capsules containing herb extract of Kaempferia parviflora has been claimed to reduce blood glucose in patients with diabetes.

Objectives

This preliminary study is conducted to evaluate the efficacy of Volten VR4® on healthy individuals and type-2 diabetes mellitus volunteers. The extracts of 400 mg capsules of Kaempferia parviflora (KP) were used to measure the blood glucose level of 2-hour postprandial.

Methods

The healthy group consists of 15 young adults aged 20-30 with no history of serious diseases, while the diabetic group includes 12 individuals aged 35-75 diagnosed with type-2 diabetes mellitus. Data were validated through the Willcoxon and Friedman test statistics and error distribution. The investigation was continued to trace the capsules contents of elements using inductively coupled plasma optical emission spectrometry (ICP-OES) techniques.

Results

It has been shown that KP reducing blood sugar levels has been associated with flavonoids and methoxyflavones components. The result specifically showed that consuming VR4® capsules can significantly reduce blood glucose, either at the state of fasting or postprandially. In the study the content of mineral and heavy metal elements in VR4® capsules has been evaluated.

Conclusion

Volten VR4® Kaempferia parviflora extract is safe to be consumed at a single dose of 400 mg. The study also has shown that the participants are free from adverse reactions and hypoglycaemia.

Keywords

  • Kaempferia parviflora
  • blood glucose
  • type-2 diabetes mellitus
  • flavonoids
Open Access

Occurrence of pathogenic fungi on commercially available medicinal plants and packaged seeds

Published Online: 02 Feb 2022
Page range: 54 - 59

Abstract

Summary Introduction

Pathogenic fungi decrease the quality of commercially available medicinal plants and packaged seeds. Some of fungal pathogens can produce mycotoxins harmful to human health.

Objective

To evaluate the health status of commercially available medicinal plants and check if packaged seeds of these plants are free from pathogenic fungi.

Methods

An experiment was conducted on potted plants of lemon balm, peppermint and sweet basil, commercially available and intended for direct use by consumers. For microbiological analyses, 100 stems and leaves of each species were randomly taken. Furthermore, the health of 100 seeds of each species was examined using agar plate method.

Results

Ten species and genera of pathogenic fungi were isolated from shoots, leaves and seeds of investigated plants. Some of them are toxin-forming species. Lemon balm was the most infected species.

Conclusion

Bearing in mind the health of consumers, more attention should be paid to the microbiological purity of commercially available medicinal plants and packaged seeds.

Keywords

  • Lamiaceae
  • mycotoxins
  • Melissa officinalis
  • Mentha piperita
  • Ocimum basilicum
  • pathogenic fungi
Open Access

Kinetic study on peroxidase inactivation and anthocyanin degradation of black cherry tomatoes (Solanum lycopersicum cv. OG) during blanching

Published Online: 02 Feb 2022
Page range: 60 - 69

Abstract

Summary Introduction

Blanching is a necessary treatment stage in processing of tomato products to inactivate enzymes. However, it may cause the degradation of nutrients.

Objective

In this study, the kinetics of thermal peroxidase inactivation and anthocyanin degradation in black cherry tomatoes (cv. OG) were determined to predict the quality changes during the blanching.

Methods

Tomatoes were blanched at five levels of temperature (75–95oC) for five time periods (30–150 s).

Results

It was found that as the blanching temperature increased and the blanching time is prolonged, more peroxidase was inactivated and the greater number of anthocyanins was lost. The thermal peroxidase inactivation and anthocyanin degradation showed an apparent first-order reaction with the activation energy of 129.96 kJ/mol and 65.99 kJ/mol, respectively. Peroxidase and anthocyanin in black cherry tomatoes were found to be heat-sensitive.

Conclusion

These kinetic parameters were necessary to select and design appropriate blanching conditions for black cherry tomatoes on larger scale processing.

Keywords

  • anthocyanin
  • black cherry tomatoes
  • blanching
  • kinetic study
  • peroxidase
  • Solanum lycopersicum cv. OG
Open Access

Selection of duplicates of flax accessions – an important task in the management of collection of genetic resources of Linum usitatissimum L.

Published Online: 02 Feb 2022
Page range: 70 - 76

Abstract

Summary Introduction

The Institute of Natural Fibres and Medicinal Plants National Research Institute (IWNiRZ) – formerly Institute of Natural Fibres, together with Plant Breeding and Acclimatization Institute (IHAR) in Radzików carry out tasks related to the protection of genetic resources of flax (Linum usitatissimum L.). Genetic resources of flax are stored as seeds in IHAR refrigeration chambers. The research material consisted of passport data of genetic resources of flax.

Objective

The aim of the presenting the identification of duplicates of accessions of flax in documentation of the National Center of Plant Genetic Resources (IHAR). This will facilitate the management of the collection of flax genetic resources. Removal of duplicate accessions from the collection of flax genetic resources will save storage space in long-term chambers, savings in storage costs, savings in working hours.

Methods

The working methods were studying the passport database and the institute’s gene bank documentation.

Results

In the cooling chambers of the seed bank at KCRZG at IHAR, unintentional duplicates are stored: 29 flax accessions with the same accessions number and 19 flax accessions with two different accession numbers.

Conclusions

The presentation in the article of two types of duplicates of flax accessions is very helpful in managing the collection of genetic resources of flax. Reducing the number of genotypes in the flax collection will facilitate the faster collection of all valorization data, which will be very helpful for users of the collection of genetic resources of flax.

Keywords

  • genetic resources
  • flax
  • Linum usitatissimum L.
  • duplicates of accessions
  • collection management
Open Access

Probiotics in prevention and treatment of cardiovascular diseases

Published Online: 02 Feb 2022
Page range: 77 - 85

Abstract

Summary

Increasing knowledge of the gut microbiota and its interference in human homeostasis in recent years has contributed to a better understanding of number of different interactions occurring in the gastrointestinal tract. Disruption of the microbiota is detrimental to health and contributes to the development of numerous diseases and may also be an accelerator of pathophysiological processes such as atherosclerosis. Cardiovascular diseases are the most common cause of death worldwide, so the development of new methods to support the treatment and prevention of these diseases becoms one of the priorities of modern medicine. Probiotics may constitute an important element of support in the treatment and prevention of CVD (cardiovascular diseases). A number of papers support such a statement, however, larger clinical trials are needed. Through a number of mechanisms including mitigating inflammation, sealing the intestinal epithelium, and affecting metabolism, probiotics may have a beneficial effect on general health and slow down the pathogenesis of many diseases, including those affecting the cardiovascular system. This article contains a review of current discoveries on the role of probiotics in the prevention and support of CVD treatment.

Keywords

  • probiotics
  • gastrointestinal diseases
  • microbiota

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