Issues

Journal & Issues

Volume 68 (2022): Issue 2 (June 2022)

Volume 68 (2022): Issue 1 (March 2022)

Volume 67 (2021): Issue 4 (December 2021)

Volume 67 (2021): Issue 3 (September 2021)

Volume 67 (2021): Issue 2 (June 2021)

Volume 67 (2021): Issue 1 (March 2021)

Volume 66 (2020): Issue 4 (December 2020)

Volume 66 (2020): Issue 3 (September 2020)

Volume 66 (2020): Issue 2 (June 2020)

Volume 66 (2020): Issue 1 (March 2020)

Volume 65 (2019): Issue 4 (December 2019)

Volume 65 (2019): Issue 3 (September 2019)

Volume 65 (2019): Issue 2 (June 2019)

Volume 65 (2019): Issue 1 (March 2019)

Volume 64 (2018): Issue 4 (December 2018)

Volume 64 (2018): Issue 3 (September 2018)

Volume 64 (2018): Issue 2 (June 2018)

Volume 64 (2018): Issue 1 (March 2018)

Volume 63 (2017): Issue 4 (December 2017)

Volume 63 (2017): Issue 3 (September 2017)

Volume 63 (2017): Issue 2 (June 2017)

Volume 63 (2017): Issue 1 (March 2017)

Volume 62 (2016): Issue 4 (December 2016)

Volume 62 (2016): Issue 3 (September 2016)

Volume 62 (2016): Issue 2 (June 2016)

Volume 62 (2016): Issue 1 (March 2016)

Volume 61 (2015): Issue 4 (December 2015)

Volume 61 (2015): Issue 3 (September 2015)

Volume 61 (2015): Issue 2 (June 2015)

Volume 61 (2015): Issue 1 (March 2015)

Volume 60 (2014): Issue 4 (December 2014)

Volume 60 (2014): Issue 3 (September 2014)

Volume 60 (2014): Issue 2 (June 2014)

Volume 60 (2014): Issue 1 (March 2014)

Volume 59 (2013): Issue 4 (December 2013)

Volume 59 (2013): Issue 3 (September 2013)

Volume 59 (2013): Issue 2 (June 2013)

Volume 59 (2013): Issue 1 (March 2013)

Journal Details
Format
Journal
eISSN
2449-8343
First Published
04 Apr 2014
Publication timeframe
4 times per year
Languages
English

Search

Volume 62 (2016): Issue 3 (September 2016)

Journal Details
Format
Journal
eISSN
2449-8343
First Published
04 Apr 2014
Publication timeframe
4 times per year
Languages
English

Search

6 Articles
Open Access

Chemical variability of common skullcap (Scutellaria galericulata L.) wild growing in the area of eastern Poland

Published Online: 08 Oct 2016
Page range: 7 - 19

Abstract

Summary

Introduction. In the group of plants revealing adaptogenic activity, species belonging to Scutellaria genus are considered to be the most important ones. Common skullcap (Scutellaria galericulata L.) is less known species from this genus, growing wild in Poland in wet habitats. Its herb has been used in the past as a sedative and diuretic agent.

Objective. The aim of the study was to determine the variability of common skullcap in terms of the accumulation of flavonoids in the herb.

Methods. The objects of the study were 17 common skullcap populations growing wild in eastern Poland. At each natural site, the list of main flowering plant species was carried out, according to Braun-Blanquet scale of plant abundance. The total content of flavonoids was determined according to EP 8th. The analysis of flavonoids was carried out using HPLC, Shimadzu chromatograph.

Results. The total content of flavonoids ranged from 0.21 to 0.50% dry matter (DM). Using HPLC, eight compounds were identified within the group, with chrysin-7-O-glucuronide (30.91–589.27 mg × 100 g−1DM), baicalin (61.90–482.93 mg × 100 g−1DM) and scutellarin (43.77–213.54 mg × 100 g−1DM) as dominants. Chrysin was the compound which in the highest degree differentiated investigated populations (CV=0.77).

Conclusion. Obtained results indicate that investigated common skullcap populations differed in terms of the total content of flavonoids as well as the content of indentified flavonoids in the herb. However, there was no clear relationship between geographical localization of populations and the content of identified flavonoids in raw material.

Keywords

  • populations
  • herb
  • flavonoids
  • baicalin
  • scutellarin
  • chrysin
Open Access

Comparison of chemical composition of the essential oils from different parts of Thuja occidentalis L. ‘Brabant’ and T. occidentalis L. ‘Smaragd’

Published Online: 08 Oct 2016
Page range: 20 - 27

Abstract

Summary

Introduction:Thuja occidentalis ‘Brabant’ and T. occidentalis ‘Smaragd’ are widely planted as decoration in parks and as hedges in domestic gardens in Poland.

Objective: The aim of the study was to determine the yield and the composition of essential oils from different parts of these cultivars.

Methods: The essential oils were obtained by hydrodistillation, and then analyzed by GC/MS.

Results: Over seventy compounds were identified. The oils from leaves, twigs with leaves and twigs without leaves of ‘Brabant’ variety contained mainly α-thujone (61.0–64.4%), β-thujone (10.4–10.7%) and fenchone (7.7–8.0%), whereas in the cone oil there were α-thujone (42.1%), sabinene (11.2%) and β-thujone (10.2%). The main constituents of the oil from leaves and twigs with leaves of ‘Smaragd’ variety were α-thujone (49.2–49.3%), beyerene (12.8–13.2%) and sabinene (8.9–9.3%).

Conclusions: The yield and chemical composition of the essential oil of T. occidentalis depend on the variety and plant organ. The oil of ‘Brabant’ variety contained more thujones and less diterpenes in comparison with the oil of ‘Smaragd’ variety.

Keywords

  • Thuja occidentalis ‘Brabant’
  • Thuja occidentalis ‘Smaragd’
  • essential oil composition
  • α-thujone
  • β-thujone
  • beyerene
Open Access

Application of multi-shoots cultures in micropropagation of willow herb (Chamaenerion angustifolium (L.) Scop.)

Published Online: 08 Oct 2016
Page range: 28 - 39

Abstract

Summary

Introduction: Willow herb (Chamaenerion angustifolium (L.) Scop. syn. Epilobium angustifolium L. from Onagraceae family is a valuable medicinal plant that has been used in the treatment of urogenital disorders including BPH (Benign Prostatic Hypertrophy). The raw material is a rich source of polyphenols as well as steroids, triterpenoids and fatty acids. Due to frequent interspecific hybridization, plants collected in wild display a very diverse and variable content of active compounds. This poses a challenge in obtaining high quality and homogenous raw material. Application of the in vitro cultures and micropropagation techniques may offer a solution for alternative methods of cultivation.

Objective: This work presents preliminary results of the first implementation of in vitro cultures of willow herb to obtain material for medicinal purposes.

Methods: Sterile seedlings were donors of explants, which were used for induction of multi-shoots culture according to a modified protocol described by Turker and co-workers. Statistical analysis was used for assessment of significance of differences among variables.

Results: Six different genotypes (lines) originating from root explants were chosen for clonal propagation. Efficiency of the elaborated method was 16–20 shoots per explant. Finally, over 3000 acclimatized plants were obtained and used for field crops.

Conclusions: The use of Ch. angustifolium in vitro cultures can contribute to the introduction of this valuable herb for field crops and increase the availability of the raw material for food and pharmaceutical industries.

Keywords

  • willow herb
  • in vitro culture
  • micropropagation
  • multi-shoots cultures
Open Access

Development of novel plant-based adjuvant formulation against rubella and hepatitis B vaccine antigen

Published Online: 08 Oct 2016
Page range: 40 - 48

Abstract

Summary

Introduction: Numerous metabolites present in the aqueous extract from plants are responsible for inducing adjuvant activity against rubella and hepatitis B vaccine antigen (HBsAg). One of the medicinal plants, Adhatoda vasica has been pointed out with great potential of vaccine adjuvant property.

Objective: The objective of our study is to evaluate the adjuvant potential of aqueous leaves extract of Adhatoda vasica against rubella and hepatitis B vaccine antigen (HBsAg).

Methods: For these studies, our group evaluated the antibody (IgG) titre of HBsAg and rubella vaccine antigen using variable doses (0.625–5 mg) of aqueous leaves extract of Adhatoda vasica and also determined the lymphocyte (splenocyte) proliferation assay (0.625–5 mg; 50 μl) in mice model studies ex vivo (i.e. immunized with HBsAg subcutaneously).

Results: The results showed that aqueous leaves extract showed anti-HBsAg and anti-rubella titre and also enhanced the lymphocyte proliferation assay at higher doses (5 mg) as compared to control.

Conclusion: Aqueous leaves extract of Adhatoda vasica showed adjuvant activity against HBsAg and rubella vaccine antigen.

Keywords

  • Adhatoda vasica
  • hepatitis B vaccine
  • rubella vaccine
  • adjuvant
Open Access

Morphological and pharmacokinetic properties of oral solid dietary supplements containing plant extracts

Published Online: 08 Oct 2016
Page range: 49 - 62

Abstract

Summary

Introduction: Dietary supplements are a good way to supplement the deficiency of certain micronutrients and organic components (therapeutic agents) in human body. They are most often available in concentrated form as tablets, capsules, powder or liquid.

Objective: To investigate morphological parameters and the pharmaceutical availability of coated tablets – dietary supplements – that contain selected pharmacopeial titrated dry plant extracts.

Methods: Testing of the effective time of the tablet surface erosion was performed in model acceptor fluids using pharmacopeial methods in static (Erweka apparatus) and dynamic (unlimited diffusion method) conditions. Furthermore, morphological parameters of tablets (the original shape of an ellipse) as well as their hardness were determined.

Results: The effective erosion time was determined by conductometric method using carboxymethylcellulose sodium salt (NaCMC) contained in the tablet. The content of gum arabic and NaCMC in the tablet testifies that the granulate was produced using the “wet granulation” technique which resulted in high hardness of original, esthetic, elliptical tablets and in prolonged disintegration time (erosion).

Conclusions: The used excipients: gum arabic and NaCMC for the production of the tested tablets containing selected dry plant extracts result in their high hardness. The tested dietary supplements are characterized by esthetic design, original shape, and prolonged disintegration time which affects the pharmaceutical availability.

Keywords

  • dietary supplements
  • extracts
  • erosion rate
  • pharmaceutical availability
  • flavonoids
  • coated tablets
Open Access

Medicinal properties of fungi occurring on Betula sp. trees. A review

Published Online: 08 Oct 2016
Page range: 63 - 76

Abstract

Summary

The article presents the chemical costituents and pharmacological properties of polyporoid fungi found on birch, namely Piptoporus betulinus, Inonotus obliquus, Lenzites betulina, Fomes fomentarius, and Trametes versicolor. The in vitro and in vivo studies on the effect of different extracts from above-mentioned fungi on the human organism shown anti-cancer, anti-inflammatory, antiviral, antibacterial and immunostimulant activity, conditioned by the presence of such compounds as polysaccharides, polyphenols or terpenes. These fungi are commonly found in Poland and may superbly compete with Ganoderma lucidum (Reishi) or Lentinula edodes (Shitake) used in Asia for medicinal purposes.

Keywords

  • polypore fungi fruiting bodies
  • Piptoporus betulinus
  • Inonotus obliquus
  • Lenzites betulina
  • Fomes fomentarius
  • Trametes versicolor
6 Articles
Open Access

Chemical variability of common skullcap (Scutellaria galericulata L.) wild growing in the area of eastern Poland

Published Online: 08 Oct 2016
Page range: 7 - 19

Abstract

Summary

Introduction. In the group of plants revealing adaptogenic activity, species belonging to Scutellaria genus are considered to be the most important ones. Common skullcap (Scutellaria galericulata L.) is less known species from this genus, growing wild in Poland in wet habitats. Its herb has been used in the past as a sedative and diuretic agent.

Objective. The aim of the study was to determine the variability of common skullcap in terms of the accumulation of flavonoids in the herb.

Methods. The objects of the study were 17 common skullcap populations growing wild in eastern Poland. At each natural site, the list of main flowering plant species was carried out, according to Braun-Blanquet scale of plant abundance. The total content of flavonoids was determined according to EP 8th. The analysis of flavonoids was carried out using HPLC, Shimadzu chromatograph.

Results. The total content of flavonoids ranged from 0.21 to 0.50% dry matter (DM). Using HPLC, eight compounds were identified within the group, with chrysin-7-O-glucuronide (30.91–589.27 mg × 100 g−1DM), baicalin (61.90–482.93 mg × 100 g−1DM) and scutellarin (43.77–213.54 mg × 100 g−1DM) as dominants. Chrysin was the compound which in the highest degree differentiated investigated populations (CV=0.77).

Conclusion. Obtained results indicate that investigated common skullcap populations differed in terms of the total content of flavonoids as well as the content of indentified flavonoids in the herb. However, there was no clear relationship between geographical localization of populations and the content of identified flavonoids in raw material.

Keywords

  • populations
  • herb
  • flavonoids
  • baicalin
  • scutellarin
  • chrysin
Open Access

Comparison of chemical composition of the essential oils from different parts of Thuja occidentalis L. ‘Brabant’ and T. occidentalis L. ‘Smaragd’

Published Online: 08 Oct 2016
Page range: 20 - 27

Abstract

Summary

Introduction:Thuja occidentalis ‘Brabant’ and T. occidentalis ‘Smaragd’ are widely planted as decoration in parks and as hedges in domestic gardens in Poland.

Objective: The aim of the study was to determine the yield and the composition of essential oils from different parts of these cultivars.

Methods: The essential oils were obtained by hydrodistillation, and then analyzed by GC/MS.

Results: Over seventy compounds were identified. The oils from leaves, twigs with leaves and twigs without leaves of ‘Brabant’ variety contained mainly α-thujone (61.0–64.4%), β-thujone (10.4–10.7%) and fenchone (7.7–8.0%), whereas in the cone oil there were α-thujone (42.1%), sabinene (11.2%) and β-thujone (10.2%). The main constituents of the oil from leaves and twigs with leaves of ‘Smaragd’ variety were α-thujone (49.2–49.3%), beyerene (12.8–13.2%) and sabinene (8.9–9.3%).

Conclusions: The yield and chemical composition of the essential oil of T. occidentalis depend on the variety and plant organ. The oil of ‘Brabant’ variety contained more thujones and less diterpenes in comparison with the oil of ‘Smaragd’ variety.

Keywords

  • Thuja occidentalis ‘Brabant’
  • Thuja occidentalis ‘Smaragd’
  • essential oil composition
  • α-thujone
  • β-thujone
  • beyerene
Open Access

Application of multi-shoots cultures in micropropagation of willow herb (Chamaenerion angustifolium (L.) Scop.)

Published Online: 08 Oct 2016
Page range: 28 - 39

Abstract

Summary

Introduction: Willow herb (Chamaenerion angustifolium (L.) Scop. syn. Epilobium angustifolium L. from Onagraceae family is a valuable medicinal plant that has been used in the treatment of urogenital disorders including BPH (Benign Prostatic Hypertrophy). The raw material is a rich source of polyphenols as well as steroids, triterpenoids and fatty acids. Due to frequent interspecific hybridization, plants collected in wild display a very diverse and variable content of active compounds. This poses a challenge in obtaining high quality and homogenous raw material. Application of the in vitro cultures and micropropagation techniques may offer a solution for alternative methods of cultivation.

Objective: This work presents preliminary results of the first implementation of in vitro cultures of willow herb to obtain material for medicinal purposes.

Methods: Sterile seedlings were donors of explants, which were used for induction of multi-shoots culture according to a modified protocol described by Turker and co-workers. Statistical analysis was used for assessment of significance of differences among variables.

Results: Six different genotypes (lines) originating from root explants were chosen for clonal propagation. Efficiency of the elaborated method was 16–20 shoots per explant. Finally, over 3000 acclimatized plants were obtained and used for field crops.

Conclusions: The use of Ch. angustifolium in vitro cultures can contribute to the introduction of this valuable herb for field crops and increase the availability of the raw material for food and pharmaceutical industries.

Keywords

  • willow herb
  • in vitro culture
  • micropropagation
  • multi-shoots cultures
Open Access

Development of novel plant-based adjuvant formulation against rubella and hepatitis B vaccine antigen

Published Online: 08 Oct 2016
Page range: 40 - 48

Abstract

Summary

Introduction: Numerous metabolites present in the aqueous extract from plants are responsible for inducing adjuvant activity against rubella and hepatitis B vaccine antigen (HBsAg). One of the medicinal plants, Adhatoda vasica has been pointed out with great potential of vaccine adjuvant property.

Objective: The objective of our study is to evaluate the adjuvant potential of aqueous leaves extract of Adhatoda vasica against rubella and hepatitis B vaccine antigen (HBsAg).

Methods: For these studies, our group evaluated the antibody (IgG) titre of HBsAg and rubella vaccine antigen using variable doses (0.625–5 mg) of aqueous leaves extract of Adhatoda vasica and also determined the lymphocyte (splenocyte) proliferation assay (0.625–5 mg; 50 μl) in mice model studies ex vivo (i.e. immunized with HBsAg subcutaneously).

Results: The results showed that aqueous leaves extract showed anti-HBsAg and anti-rubella titre and also enhanced the lymphocyte proliferation assay at higher doses (5 mg) as compared to control.

Conclusion: Aqueous leaves extract of Adhatoda vasica showed adjuvant activity against HBsAg and rubella vaccine antigen.

Keywords

  • Adhatoda vasica
  • hepatitis B vaccine
  • rubella vaccine
  • adjuvant
Open Access

Morphological and pharmacokinetic properties of oral solid dietary supplements containing plant extracts

Published Online: 08 Oct 2016
Page range: 49 - 62

Abstract

Summary

Introduction: Dietary supplements are a good way to supplement the deficiency of certain micronutrients and organic components (therapeutic agents) in human body. They are most often available in concentrated form as tablets, capsules, powder or liquid.

Objective: To investigate morphological parameters and the pharmaceutical availability of coated tablets – dietary supplements – that contain selected pharmacopeial titrated dry plant extracts.

Methods: Testing of the effective time of the tablet surface erosion was performed in model acceptor fluids using pharmacopeial methods in static (Erweka apparatus) and dynamic (unlimited diffusion method) conditions. Furthermore, morphological parameters of tablets (the original shape of an ellipse) as well as their hardness were determined.

Results: The effective erosion time was determined by conductometric method using carboxymethylcellulose sodium salt (NaCMC) contained in the tablet. The content of gum arabic and NaCMC in the tablet testifies that the granulate was produced using the “wet granulation” technique which resulted in high hardness of original, esthetic, elliptical tablets and in prolonged disintegration time (erosion).

Conclusions: The used excipients: gum arabic and NaCMC for the production of the tested tablets containing selected dry plant extracts result in their high hardness. The tested dietary supplements are characterized by esthetic design, original shape, and prolonged disintegration time which affects the pharmaceutical availability.

Keywords

  • dietary supplements
  • extracts
  • erosion rate
  • pharmaceutical availability
  • flavonoids
  • coated tablets
Open Access

Medicinal properties of fungi occurring on Betula sp. trees. A review

Published Online: 08 Oct 2016
Page range: 63 - 76

Abstract

Summary

The article presents the chemical costituents and pharmacological properties of polyporoid fungi found on birch, namely Piptoporus betulinus, Inonotus obliquus, Lenzites betulina, Fomes fomentarius, and Trametes versicolor. The in vitro and in vivo studies on the effect of different extracts from above-mentioned fungi on the human organism shown anti-cancer, anti-inflammatory, antiviral, antibacterial and immunostimulant activity, conditioned by the presence of such compounds as polysaccharides, polyphenols or terpenes. These fungi are commonly found in Poland and may superbly compete with Ganoderma lucidum (Reishi) or Lentinula edodes (Shitake) used in Asia for medicinal purposes.

Keywords

  • polypore fungi fruiting bodies
  • Piptoporus betulinus
  • Inonotus obliquus
  • Lenzites betulina
  • Fomes fomentarius
  • Trametes versicolor

Plan your remote conference with Sciendo