Issues

Journal & Issues

Volume 68 (2022): Issue 2 (June 2022)

Volume 68 (2022): Issue 1 (March 2022)

Volume 67 (2021): Issue 4 (December 2021)

Volume 67 (2021): Issue 3 (September 2021)

Volume 67 (2021): Issue 2 (June 2021)

Volume 67 (2021): Issue 1 (March 2021)

Volume 66 (2020): Issue 4 (December 2020)

Volume 66 (2020): Issue 3 (September 2020)

Volume 66 (2020): Issue 2 (June 2020)

Volume 66 (2020): Issue 1 (March 2020)

Volume 65 (2019): Issue 4 (December 2019)

Volume 65 (2019): Issue 3 (September 2019)

Volume 65 (2019): Issue 2 (June 2019)

Volume 65 (2019): Issue 1 (March 2019)

Volume 64 (2018): Issue 4 (December 2018)

Volume 64 (2018): Issue 3 (September 2018)

Volume 64 (2018): Issue 2 (June 2018)

Volume 64 (2018): Issue 1 (March 2018)

Volume 63 (2017): Issue 4 (December 2017)

Volume 63 (2017): Issue 3 (September 2017)

Volume 63 (2017): Issue 2 (June 2017)

Volume 63 (2017): Issue 1 (March 2017)

Volume 62 (2016): Issue 4 (December 2016)

Volume 62 (2016): Issue 3 (September 2016)

Volume 62 (2016): Issue 2 (June 2016)

Volume 62 (2016): Issue 1 (March 2016)

Volume 61 (2015): Issue 4 (December 2015)

Volume 61 (2015): Issue 3 (September 2015)

Volume 61 (2015): Issue 2 (June 2015)

Volume 61 (2015): Issue 1 (March 2015)

Volume 60 (2014): Issue 4 (December 2014)

Volume 60 (2014): Issue 3 (September 2014)

Volume 60 (2014): Issue 2 (June 2014)

Volume 60 (2014): Issue 1 (March 2014)

Volume 59 (2013): Issue 4 (December 2013)

Volume 59 (2013): Issue 3 (September 2013)

Volume 59 (2013): Issue 2 (June 2013)

Volume 59 (2013): Issue 1 (March 2013)

Journal Details
Format
Journal
eISSN
2449-8343
First Published
04 Apr 2014
Publication timeframe
4 times per year
Languages
English

Search

Volume 61 (2015): Issue 1 (March 2015)

Journal Details
Format
Journal
eISSN
2449-8343
First Published
04 Apr 2014
Publication timeframe
4 times per year
Languages
English

Search

5 Articles
Open Access

Cardinal temperatures for germination of Salvia leriifolia Benth

Published Online: 18 Apr 2015
Page range: 5 - 18

Abstract

Summary

The focus of this study is based on the examination of the germination traits and the development of thermal models of the medicinal plant Salvia leriifolia Benth. A laboratory experiment was carried out at constant temperatures ranging from 0 to 35°C, at 5°C intervals in a completely randomized design with eight replications. To describe the germination rate response to temperature, three regression models, namely Intersected-Lines (ISL), Quadratic Polynomial (QPN) and Five-Parameters Beta (FPB) were used. The highest Germination Percentage (GP) (92.8%) occurred in 15°C, but GP in the range of 10-25°C was not significant (p≤0.05). The germination process stopped at 0°C and at above 30°C. The results indicated that the highest Germination Rate (GR), the lowest Mean Germination Time (MGT) and also times to 50% germination (D50) were obtained at 20°C. Seeds did not reach to their 50% germination level in temperatures higher than 25°C. The FPB model had the best realistic estimation for cardinal temperatures. Based on models estimation, Base (Tb), Optimum (To) and Ceiling (Tc) temperatures were in the ranges of (1-1.9°C), (18.1-20.8°C) and (34.5-38.7°C), respectively.

Keywords

  • cardinal temperatures
  • medicinal plants
  • Salvia leriifolia Benth.
  • regression models
Open Access

Analysis of accumulation of cadmium in seeds of selected breeding linseed (Linum usitatissimum L.) genotypes cultivated for medicinal purposes

Published Online: 18 Apr 2015
Page range: 19 - 30

Abstract

Summary

The objective of the study was genetic and breeding screening of selected varieties and hybrids of oil flax for in-seed cadmium fixing ability. Seven parental varieties as well as nineteen F1 and F2 hybrids were analyzed. The varieties were crossed in di-allelic scheme, according to the Griffing method II. Significant differences were found in the activity of fixing cadmium from the soil between analysed flax parental forms and hybrids. High variability of tested genotypes, calculated by the variance coefficient, was observed in F1 generation. The variability of tested genotypes in the F2 generation was two times lower. The analysis of general combining ability (GCA) showed that Chinese cultivar Shanxi reduced significantly the fixing cadmium from the soil in combinations of F1 generation crossing. However, the effects of the specific combining ability (SCA) of tested hybrids to reduce the cadmium content in seeds were statistically insignificant. The analysis of genetic components variance showed no significance both in domination and cumulative genes which suggests a complex inheritance pattern of the tested trait.

Keywords

  • Linum usitatissimum
  • cadmium accumulation
  • breeding
Open Access

The antimicrobial activity of Prunella vulgaris extracts

Published Online: 18 Apr 2015
Page range: 31 - 38

Abstract

Summary

Prunella vulgaris ( Labiatae family) or self-heal is traditionally used for different ailments such as eye pain and inflammation, headache, dizziness, sore throat and wound healing. Total phenolic and total flavonoid contents of extracts (methanol, ethanol and aqueous) were determined by a spectrophotometer. The antimicrobial activity was evaluated by micro broth dilution assay. The total phenolic content of P. vulgaris extracts were higher in aqueous extract (156.5 mg GAC/g) followed by ethanol extract and methanol extract. The TFC content of P. vulgaris methanol extract (82.8 mg QE/g) was higher than ethanol extract (22.7 mg QE/g) and aqueous extract (16.2 mg QE/g). The antimicrobial activity of methanol or ethanol extracts was higher than aqueous extract from P. vulgaris. The sensitivity of microorganisms to different extracts is related to type of pathogens. There is no positive relation between total phenolic content and its antimicrobial activity. Prunella vulgaris ethanolic extract as a source of phenolic and flavonoid contents can be used as an antimicrobial agent.

Keywords

  • Prunella vulgaris
  • antimicrobial activity
  • phenolic
  • flavonoid
  • extract
Open Access

Antimicrobial activity of Ficus sycomorus L. (Moraceae) leaf and stem-bark extracts against multidrug resistant human pathogens

Published Online: 18 Apr 2015
Page range: 39 - 49

Abstract

Summary

The present work was conducted to investigate antibacterial activity of methanol and acetone in leaf (LE) and stem-bark (SBE) of Ficus sycomorus L. crude extracts against sensitive and resistant species of Staphylococcus aureus and Acinetobacter baumannii pathogens. Antimicrobial activity expressed by disc-diffusion method (zone of inhibitions - ZIs), minimum inhibitory concentrations (MICs) and minimum bactericidal concentration (MBC) were measured as reported for many investigations. Similar study with 6 commercial antibiotics as a reference drug was undertaken. Based upon the estimated ZIs, MIC and MBC values, acetone LE exhibited higher antimicrobial activity than that of methanol one. Otherwise, standard antibiotics have lower effectiveness (ZIs, MICs and MBC) on all tested bacteria as compared to the SBE and LE. The highest antibacterial activity was recorded in sensitive A. baumannii isolate with MICs 2.5, 4.9 mg/ml and MBC 3.8, 9.7 mg/ml for acetone LE and SBE, respectively. Our data indicated that the lowest antibiotics antibacterial activity was recorded for resistant A. baumannii pathogen. It was lower than those of the both plant fractions extracts.

Keywords

  • antibacterial activity
  • Ficus sycomorus L.
  • minimum inhibitory concentrations (MICs)
  • minimum bactericidal concentration (MBC)
Open Access

Application of in vitro stevia (Stevia rebaudiana Bertoni) cultures in obtaining steviol glycoside rich material

Published Online: 18 Apr 2015
Page range: 50 - 63

Abstract

Summary

Stevia is a plant attracting attention due to its capability to synthesize a group of chemical compounds with sweet taste, i.e. steviol glycosides. Steviol glycosides are successfully applied as a natural sweetener, and some of them have also therapeutic properties. This paper presents available information on the use of stevia plant tissue cultures with the focus on their potential application in food industry. Detailed analysis was done concerning the research employing in vitro culture techniques and the use of them in biosynthesis of secondary metabolites of high importance for the food industry. Both established achievements and most recent publications on stevia were used for assessment of practical applications of the aforementioned techniques and prospects for their development.

Keywords

  • steviol glycosides
  • in vitro cultures
  • secondary metabolites
  • micropropagation
  • Stevia rebaudiana
  • stevia
  • stevioside
5 Articles
Open Access

Cardinal temperatures for germination of Salvia leriifolia Benth

Published Online: 18 Apr 2015
Page range: 5 - 18

Abstract

Summary

The focus of this study is based on the examination of the germination traits and the development of thermal models of the medicinal plant Salvia leriifolia Benth. A laboratory experiment was carried out at constant temperatures ranging from 0 to 35°C, at 5°C intervals in a completely randomized design with eight replications. To describe the germination rate response to temperature, three regression models, namely Intersected-Lines (ISL), Quadratic Polynomial (QPN) and Five-Parameters Beta (FPB) were used. The highest Germination Percentage (GP) (92.8%) occurred in 15°C, but GP in the range of 10-25°C was not significant (p≤0.05). The germination process stopped at 0°C and at above 30°C. The results indicated that the highest Germination Rate (GR), the lowest Mean Germination Time (MGT) and also times to 50% germination (D50) were obtained at 20°C. Seeds did not reach to their 50% germination level in temperatures higher than 25°C. The FPB model had the best realistic estimation for cardinal temperatures. Based on models estimation, Base (Tb), Optimum (To) and Ceiling (Tc) temperatures were in the ranges of (1-1.9°C), (18.1-20.8°C) and (34.5-38.7°C), respectively.

Keywords

  • cardinal temperatures
  • medicinal plants
  • Salvia leriifolia Benth.
  • regression models
Open Access

Analysis of accumulation of cadmium in seeds of selected breeding linseed (Linum usitatissimum L.) genotypes cultivated for medicinal purposes

Published Online: 18 Apr 2015
Page range: 19 - 30

Abstract

Summary

The objective of the study was genetic and breeding screening of selected varieties and hybrids of oil flax for in-seed cadmium fixing ability. Seven parental varieties as well as nineteen F1 and F2 hybrids were analyzed. The varieties were crossed in di-allelic scheme, according to the Griffing method II. Significant differences were found in the activity of fixing cadmium from the soil between analysed flax parental forms and hybrids. High variability of tested genotypes, calculated by the variance coefficient, was observed in F1 generation. The variability of tested genotypes in the F2 generation was two times lower. The analysis of general combining ability (GCA) showed that Chinese cultivar Shanxi reduced significantly the fixing cadmium from the soil in combinations of F1 generation crossing. However, the effects of the specific combining ability (SCA) of tested hybrids to reduce the cadmium content in seeds were statistically insignificant. The analysis of genetic components variance showed no significance both in domination and cumulative genes which suggests a complex inheritance pattern of the tested trait.

Keywords

  • Linum usitatissimum
  • cadmium accumulation
  • breeding
Open Access

The antimicrobial activity of Prunella vulgaris extracts

Published Online: 18 Apr 2015
Page range: 31 - 38

Abstract

Summary

Prunella vulgaris ( Labiatae family) or self-heal is traditionally used for different ailments such as eye pain and inflammation, headache, dizziness, sore throat and wound healing. Total phenolic and total flavonoid contents of extracts (methanol, ethanol and aqueous) were determined by a spectrophotometer. The antimicrobial activity was evaluated by micro broth dilution assay. The total phenolic content of P. vulgaris extracts were higher in aqueous extract (156.5 mg GAC/g) followed by ethanol extract and methanol extract. The TFC content of P. vulgaris methanol extract (82.8 mg QE/g) was higher than ethanol extract (22.7 mg QE/g) and aqueous extract (16.2 mg QE/g). The antimicrobial activity of methanol or ethanol extracts was higher than aqueous extract from P. vulgaris. The sensitivity of microorganisms to different extracts is related to type of pathogens. There is no positive relation between total phenolic content and its antimicrobial activity. Prunella vulgaris ethanolic extract as a source of phenolic and flavonoid contents can be used as an antimicrobial agent.

Keywords

  • Prunella vulgaris
  • antimicrobial activity
  • phenolic
  • flavonoid
  • extract
Open Access

Antimicrobial activity of Ficus sycomorus L. (Moraceae) leaf and stem-bark extracts against multidrug resistant human pathogens

Published Online: 18 Apr 2015
Page range: 39 - 49

Abstract

Summary

The present work was conducted to investigate antibacterial activity of methanol and acetone in leaf (LE) and stem-bark (SBE) of Ficus sycomorus L. crude extracts against sensitive and resistant species of Staphylococcus aureus and Acinetobacter baumannii pathogens. Antimicrobial activity expressed by disc-diffusion method (zone of inhibitions - ZIs), minimum inhibitory concentrations (MICs) and minimum bactericidal concentration (MBC) were measured as reported for many investigations. Similar study with 6 commercial antibiotics as a reference drug was undertaken. Based upon the estimated ZIs, MIC and MBC values, acetone LE exhibited higher antimicrobial activity than that of methanol one. Otherwise, standard antibiotics have lower effectiveness (ZIs, MICs and MBC) on all tested bacteria as compared to the SBE and LE. The highest antibacterial activity was recorded in sensitive A. baumannii isolate with MICs 2.5, 4.9 mg/ml and MBC 3.8, 9.7 mg/ml for acetone LE and SBE, respectively. Our data indicated that the lowest antibiotics antibacterial activity was recorded for resistant A. baumannii pathogen. It was lower than those of the both plant fractions extracts.

Keywords

  • antibacterial activity
  • Ficus sycomorus L.
  • minimum inhibitory concentrations (MICs)
  • minimum bactericidal concentration (MBC)
Open Access

Application of in vitro stevia (Stevia rebaudiana Bertoni) cultures in obtaining steviol glycoside rich material

Published Online: 18 Apr 2015
Page range: 50 - 63

Abstract

Summary

Stevia is a plant attracting attention due to its capability to synthesize a group of chemical compounds with sweet taste, i.e. steviol glycosides. Steviol glycosides are successfully applied as a natural sweetener, and some of them have also therapeutic properties. This paper presents available information on the use of stevia plant tissue cultures with the focus on their potential application in food industry. Detailed analysis was done concerning the research employing in vitro culture techniques and the use of them in biosynthesis of secondary metabolites of high importance for the food industry. Both established achievements and most recent publications on stevia were used for assessment of practical applications of the aforementioned techniques and prospects for their development.

Keywords

  • steviol glycosides
  • in vitro cultures
  • secondary metabolites
  • micropropagation
  • Stevia rebaudiana
  • stevia
  • stevioside

Plan your remote conference with Sciendo