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Volume 59 (2013): Issue 1 (March 2013)

Journal Details
Format
Journal
eISSN
2449-8343
First Published
04 Apr 2014
Publication timeframe
4 times per year
Languages
English

Search

Volume 59 (2013): Issue 1 (March 2013)

Journal Details
Format
Journal
eISSN
2449-8343
First Published
04 Apr 2014
Publication timeframe
4 times per year
Languages
English

Search

6 Articles
Open Access

Determination of chlorogenic and gallic acids by UPLC-MS/MS

Published Online: 04 Apr 2014
Page range: 7 - 16

Abstract

Summary

The aim of our study were qualitative and quantitative analyses of two polyphenolic acids: chlorogenic and gallic acids. These compounds were determined in two species of Rhodiola: R. kirilowii and R. rosea. After collecting plants, aqueous and hydroalcoholic extracts were prepared. In order to identify analysed polyphenolic compounds ultra performance liquid chromatography - tandem mass spectrometry (UPLC-MS/MS, Waters) was used. Gallic acid is commonly found in the roots of these plants. Aqueous extract in both species is a rich source of gallic acid. The UPLC-MS/MS studies allow to use this analytical method for determination of polyphenolic acids accordance with the requirements of ICH. Chromatographic method developed by our team is more precise then previously published.

Keywords

  • Rhodiola kirilowii
  • Rhodiola rosea
  • chlorogenic acid
  • gallic acid
  • UPLC-MS/MS

Słowa kluczowe

  • Rhodiola kirilowii
  • Rhodiola rosea
  • kwas chlorogenowy
  • kwas galusowy
  • UPLCMS/ MS
Open Access

Novel method of detection of phenylpropanoids of Rhodiola roots species

Published Online: 04 Apr 2014
Page range: 17 - 28

Abstract

Summary

The aim of the study was the identification and quantitative analysis of phenylpropanoid compounds in the roots of Rhodiola species. Rosavin, rosarin and rosin were determined in the roots of R. kirilowii and R. rosea from the field cultivation, Institute of Natural Fibres and Medicinal Plants. For the quantitative analysis, the ultra performance liquid chromatography - tandem mass spectrometry (UPLC-ESI MS/MS, Waters) was used. The results showed differences in the quantitative and qualitative assessments of these two species. In the root of R. kirilowii the presence of phenylpropanoids was not confirmed. In R. rosea the most common phenylpropanoid was rosavin (0.022%). The UPLC-MS/MS studies allowed to use this analytical method for determination of phenylpropanoids in the accordance with the requirements of ICH.

Keywords

  • Rhodiola kirilowii
  • Rhodiola rosea
  • phenylpropanoids
  • UPLC-MS/MS

Słowa kluczowe

  • Rhodiola kirilowii
  • Rhodiola rosea
  • UPLC-MS/MS
  • fenylopropanoidy
Open Access

Chemical composition of Scaligeria meifolia Boiss. essential oil from Iran

Published Online: 04 Apr 2014
Page range: 29 - 34

Abstract

Summary

Scaligeria meifolia Boiss., belonging to the Apiaceae family, grows wild in Iran. The essential oil from aerial parts of S. meifolia were obtained by hydrodistillation and analyzed by GC and GC/MS. Thirty-one constituents representing 99.98% of total oil components were identified. The main constituents of the essential oil were germacrene-D, (24.2%), germacrene-B (14.8%), limonene (14.2%), γ-elemene (11.6 %) and β-elemene (5.2%).

Keywords

  • Scaligeria meifolia
  • Apiaceae
  • essential oil
  • GC/MS

Słowa kluczowe

  • Scaligeria meifolia
  • Apiaceae
  • olejek eteryczny
  • GC/MS
Open Access

Phenolics in the Tussilago farfara leaves

Published Online: 04 Apr 2014
Page range: 35 - 43

Abstract

Summary

Coltsfoot leaves (Farfarae folium) are used in the European medicine in respiratory tract diseases, for cough, bronchitis and asthmatic disorders, while in the traditional Chinese medicine only flower buds (Farfarae flos) have been recognized as a medicine. A short literature review shows that most data concern the chemical composition of the coltsfoot flowers. During the carried out studies we have isolated and identified (UV, 1H and 13C NMR, analysis of acid and enzymatic hydrolyze products) six known flavonols from the coltsfoot leaves: kaempferol and its 3-O-β-glucopyranoside and 3-O-α-rhamnopyranosyl(1→6)- β-glucopyranoside, along with quercetin derivatives: 3-O-β-arabinopyranoside, 3-O-β- glucopyranoside and 3-O-α-rhamnopyranosyl(1→6)-β-glucopyranoside. Moreover, we have detected the presence of three phenolic acids.

Keywords

  • Tussilago farfara
  • leaves
  • flavonoids
  • phenolic acids

Słowa kluczowe

  • Tussilago farfara
  • liście
  • flawonoidy
  • kwasy fenolowe
Open Access

Isolation and in vitro cytotoxic activity of 11-methylixoside isolated from bark of Randia dumetorum Lamk

Published Online: 04 Apr 2014
Page range: 44 - 52

Abstract

Summary

Randia dumetorum (family Rubiaceae) is highly reputed ayurvedic medicinal tree commonly known as the Mainphal. A large deciduous thorny shrub grows up to 5 m of height. It occurs almost throughout India up to 1200 m of altitude. It is found in Himalaya from Jammu East ward ascending to 400 m and from Kashmir to East ward up to 1200 m. 11-methylixoside (compound 1), an iridoid glucoside, was isolated from the bark of this plant. The structure was characterized by using spectroscopic methods including 1D-1HNMR,13C-NMR and 2D-NMR (HSQC,HMBC, DQF-COSY) experiments and confirmed by comparison of their NMR data with those from the literature. This compound has been reported for the first time in Randia dumetorum bark. The 11-methylixoside was subjected to cytotoxic activity against MDA-MB-231 (breast cancer cell line) and SK-MEL-2 (human skin melanoma cell line), BE(2)C (neuroblastoma cell line derived from human bone marrow) and U87MG (human neuronale glioblastoma (astrozytom) cell line showed appreciable cytotoxic effect with IC50 value 63.10 µg/ml concentration for SK-MEL-2 (human skin melanoma cell line).

Keywords

  • Randia dumetorum
  • Rubiaceae
  • iridoid glucoside
  • 2D-NMR
  • cytotoxic activity

Słowa kluczowe

  • Randia dumetorum
  • Rubiaceae
  • glukozyd irydoidowy
  • 2D-NMR
  • działanie cytotoksyczne
Open Access

Phytochemical and pharmacological aspects of Nothapodytes nimmoniana. An overview

Published Online: 04 Apr 2014
Page range: 53 - 66

Abstract

Summary

Nothapodytes nimmoniana (J. Graham) (Icacinaceae), commonly known as Amruta is found in India particularly in Maharashtra, Goa, Kerala, Assam, Jammu and Kashmir as well as Tamilnadu areas. It is an important medicinal plant, the major source of a potent alkaloid, namely camptothecin, of a wide spectrum of pharmacological activities like anti-cancer, anti-HIV, antimalarial, antibacterial, anti-oxidant, anti-inflammatory, anti-fungal and also applied in the treatment of anaemia. Camptothecin is still not synthesized, therefore, its production entirely depends on natural sources. N. nimmoniana is one such plant which yields contain camptothecin in significantly high amount. The plant is gaining international recognition due to its diversified medicinal uses. It is subjected to excessive harvest. It has been categorized as a vulnerable and endangered plant. The present review encompasses the phytochemical, analytical, pharmacological, biotechnological, and other specific aspects of N. nimmoniana.

Keywords

  • Nothapodytes nimmoniana
  • camptothecin
  • pharmacological activities
  • phytochemistry
6 Articles
Open Access

Determination of chlorogenic and gallic acids by UPLC-MS/MS

Published Online: 04 Apr 2014
Page range: 7 - 16

Abstract

Summary

The aim of our study were qualitative and quantitative analyses of two polyphenolic acids: chlorogenic and gallic acids. These compounds were determined in two species of Rhodiola: R. kirilowii and R. rosea. After collecting plants, aqueous and hydroalcoholic extracts were prepared. In order to identify analysed polyphenolic compounds ultra performance liquid chromatography - tandem mass spectrometry (UPLC-MS/MS, Waters) was used. Gallic acid is commonly found in the roots of these plants. Aqueous extract in both species is a rich source of gallic acid. The UPLC-MS/MS studies allow to use this analytical method for determination of polyphenolic acids accordance with the requirements of ICH. Chromatographic method developed by our team is more precise then previously published.

Keywords

  • Rhodiola kirilowii
  • Rhodiola rosea
  • chlorogenic acid
  • gallic acid
  • UPLC-MS/MS

Słowa kluczowe

  • Rhodiola kirilowii
  • Rhodiola rosea
  • kwas chlorogenowy
  • kwas galusowy
  • UPLCMS/ MS
Open Access

Novel method of detection of phenylpropanoids of Rhodiola roots species

Published Online: 04 Apr 2014
Page range: 17 - 28

Abstract

Summary

The aim of the study was the identification and quantitative analysis of phenylpropanoid compounds in the roots of Rhodiola species. Rosavin, rosarin and rosin were determined in the roots of R. kirilowii and R. rosea from the field cultivation, Institute of Natural Fibres and Medicinal Plants. For the quantitative analysis, the ultra performance liquid chromatography - tandem mass spectrometry (UPLC-ESI MS/MS, Waters) was used. The results showed differences in the quantitative and qualitative assessments of these two species. In the root of R. kirilowii the presence of phenylpropanoids was not confirmed. In R. rosea the most common phenylpropanoid was rosavin (0.022%). The UPLC-MS/MS studies allowed to use this analytical method for determination of phenylpropanoids in the accordance with the requirements of ICH.

Keywords

  • Rhodiola kirilowii
  • Rhodiola rosea
  • phenylpropanoids
  • UPLC-MS/MS

Słowa kluczowe

  • Rhodiola kirilowii
  • Rhodiola rosea
  • UPLC-MS/MS
  • fenylopropanoidy
Open Access

Chemical composition of Scaligeria meifolia Boiss. essential oil from Iran

Published Online: 04 Apr 2014
Page range: 29 - 34

Abstract

Summary

Scaligeria meifolia Boiss., belonging to the Apiaceae family, grows wild in Iran. The essential oil from aerial parts of S. meifolia were obtained by hydrodistillation and analyzed by GC and GC/MS. Thirty-one constituents representing 99.98% of total oil components were identified. The main constituents of the essential oil were germacrene-D, (24.2%), germacrene-B (14.8%), limonene (14.2%), γ-elemene (11.6 %) and β-elemene (5.2%).

Keywords

  • Scaligeria meifolia
  • Apiaceae
  • essential oil
  • GC/MS

Słowa kluczowe

  • Scaligeria meifolia
  • Apiaceae
  • olejek eteryczny
  • GC/MS
Open Access

Phenolics in the Tussilago farfara leaves

Published Online: 04 Apr 2014
Page range: 35 - 43

Abstract

Summary

Coltsfoot leaves (Farfarae folium) are used in the European medicine in respiratory tract diseases, for cough, bronchitis and asthmatic disorders, while in the traditional Chinese medicine only flower buds (Farfarae flos) have been recognized as a medicine. A short literature review shows that most data concern the chemical composition of the coltsfoot flowers. During the carried out studies we have isolated and identified (UV, 1H and 13C NMR, analysis of acid and enzymatic hydrolyze products) six known flavonols from the coltsfoot leaves: kaempferol and its 3-O-β-glucopyranoside and 3-O-α-rhamnopyranosyl(1→6)- β-glucopyranoside, along with quercetin derivatives: 3-O-β-arabinopyranoside, 3-O-β- glucopyranoside and 3-O-α-rhamnopyranosyl(1→6)-β-glucopyranoside. Moreover, we have detected the presence of three phenolic acids.

Keywords

  • Tussilago farfara
  • leaves
  • flavonoids
  • phenolic acids

Słowa kluczowe

  • Tussilago farfara
  • liście
  • flawonoidy
  • kwasy fenolowe
Open Access

Isolation and in vitro cytotoxic activity of 11-methylixoside isolated from bark of Randia dumetorum Lamk

Published Online: 04 Apr 2014
Page range: 44 - 52

Abstract

Summary

Randia dumetorum (family Rubiaceae) is highly reputed ayurvedic medicinal tree commonly known as the Mainphal. A large deciduous thorny shrub grows up to 5 m of height. It occurs almost throughout India up to 1200 m of altitude. It is found in Himalaya from Jammu East ward ascending to 400 m and from Kashmir to East ward up to 1200 m. 11-methylixoside (compound 1), an iridoid glucoside, was isolated from the bark of this plant. The structure was characterized by using spectroscopic methods including 1D-1HNMR,13C-NMR and 2D-NMR (HSQC,HMBC, DQF-COSY) experiments and confirmed by comparison of their NMR data with those from the literature. This compound has been reported for the first time in Randia dumetorum bark. The 11-methylixoside was subjected to cytotoxic activity against MDA-MB-231 (breast cancer cell line) and SK-MEL-2 (human skin melanoma cell line), BE(2)C (neuroblastoma cell line derived from human bone marrow) and U87MG (human neuronale glioblastoma (astrozytom) cell line showed appreciable cytotoxic effect with IC50 value 63.10 µg/ml concentration for SK-MEL-2 (human skin melanoma cell line).

Keywords

  • Randia dumetorum
  • Rubiaceae
  • iridoid glucoside
  • 2D-NMR
  • cytotoxic activity

Słowa kluczowe

  • Randia dumetorum
  • Rubiaceae
  • glukozyd irydoidowy
  • 2D-NMR
  • działanie cytotoksyczne
Open Access

Phytochemical and pharmacological aspects of Nothapodytes nimmoniana. An overview

Published Online: 04 Apr 2014
Page range: 53 - 66

Abstract

Summary

Nothapodytes nimmoniana (J. Graham) (Icacinaceae), commonly known as Amruta is found in India particularly in Maharashtra, Goa, Kerala, Assam, Jammu and Kashmir as well as Tamilnadu areas. It is an important medicinal plant, the major source of a potent alkaloid, namely camptothecin, of a wide spectrum of pharmacological activities like anti-cancer, anti-HIV, antimalarial, antibacterial, anti-oxidant, anti-inflammatory, anti-fungal and also applied in the treatment of anaemia. Camptothecin is still not synthesized, therefore, its production entirely depends on natural sources. N. nimmoniana is one such plant which yields contain camptothecin in significantly high amount. The plant is gaining international recognition due to its diversified medicinal uses. It is subjected to excessive harvest. It has been categorized as a vulnerable and endangered plant. The present review encompasses the phytochemical, analytical, pharmacological, biotechnological, and other specific aspects of N. nimmoniana.

Keywords

  • Nothapodytes nimmoniana
  • camptothecin
  • pharmacological activities
  • phytochemistry

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