<abstract><title style='display:none'>Abstract</title> Infectious arthritides in goats is mainly caused by mycoplasmas and Caprine Arthritis- Encephalitis virus (CAEV). Streptococcus equi subspecies zooepidemicus (S. zooepidemicus) is a member of Lancefield group C beta-hemolytic streptococcus that commonly colonizes the mucous membranes of healthy equids which is also capable to induce different pathological conditions in many animal species. In this paper we described a case of polyarthritis in four Alpine goat-kids caused by S. zooepidemicus. Goat-kids originated from a farm where sheep, llamas, shepherd dogs, cows and horses were kept with goats. During the external examination of carcasses, crusts and discrete hyperemia were seen on the earlobes beneath the ear tag, as well as swollen joints. Necropsy findings included severe fibrinous polyarthritis in all four examined kids. Shoulder, knee and hip joints were affected. From all affected joints S. zooepidemicus was isolated. Neither Mycoplasma spp. nor CAEV genome were detected from the affected joints. Most likely the goatkids were infected with S. zooepidemicus through the lesions on earlobes which were made after the tagging. As a possible source of bacteria, we recognized the horses and dogs. Due to the lack of any signs of illness in other animal species on the farm, as well as negative pathomorphological findings in other organs, we assumed that the isolated strain is highly adapted to goat-kids. Our report is the first described case of polyarthritis in goat-kids caused by S. zooepidemicus.</abstract>

Polyarthritis in Goat-Kids Caused by Streptococcus Equi Subspecies Zooepidemicus

<abstract><title style='display:none'>Abstract</title>In this study, determination of enterococcus species that were isolated from mastitic milk samples, investigation of their susceptibilities to antibiotics and identification of the existence of resistance genes in resistant strains were conducted. The specimens consist of 600 mastitic milk samples that were collected from 242 cows. Isolation of enterococcus was carried out in selective media and 94 (15.6%) <italic>Enterococcus</italic> spp. were isolated. A total of 94 species of Enterococci were identified using both sequencing and polymerase chain reaction (PCR). <italic>Enterococcus</italic> spp. isolates belong to 5 different species (<italic>E. faecalis, E. faecium, E. durans, E. hirae, E. mundtii</italic>) in sequence analysis and 4 different species (<italic>E. faecalis, E. faecium, E. durans, E. hirae</italic>) were identify by PCR method with specific primers. Analyzing 94 enterococcus strains by antibiotic sensitiveness test a high rate of resistance to tetracycline in 77 (81.9%) isolates was shown. The <italic>tet</italic> resistance genes were identified as follows: 54 were <italic>tet</italic>M positive, 23 were <italic>tet</italic>K positive and 17 were positive on <italic>tet</italic>M and <italic>tet</italic>K. Resistance to erythromycin was established in 27 (28.7%) isolates (25 <italic>erm</italic>B) while the chloramphenicol resistance gene was found in 10 (10.7%) of isolates and the <italic>cat</italic> gene was identified in nine samples and one isolate was resistant to vancomycin (1.06%) with the <italic>Van</italic>A gene confirmed. In conclusion, it was shown that <italic>E. faecalis</italic> has the biggest role in <italic>enterococcus</italic> originated mastitis and these strains were found to be mostly resistant to tetracycline. One vancomycine resistant isolate that had the <italic>Van</italic>A gene was also determined.</abstract>

Distribution of Antibiotic Resistance Genes in <italic>Enterococcus</italic> spp. Isolated from Mastitis Bovine Milk

<abstract>
<title style='display:none'>Abstract</title>
Lumpy skin disease (LSD) is an infectious disease mostly of cattle. The typical clinical picture is usually characterized by the appearance of multiple nodules on the skin and internal organs. They can cover the entire body of the animal in the course of severe illness. This disease causes serious economic damage despite the fact that mortality of cattle with LSD is often low. Now LSD is a serious danger for cattle in the Asian part of Russia (part of the territory of the Russian Federation geographically belonging to Asia) and the Southeast Asia. Initially LSD was an endemic disease in many Sub-Saharan African countries, then it spread to Asia and Europe. In order to prevent the spread of the lumpy skin disease virus (LSDV), strict quarantine is introduced, vector control is carried out and various other LSD control measures are implemented. An effective vaccination campaign is required to significantly reduce the morbidity. However, the risk of spreading this transboundary disease to neighboring LSDV virus-free countries and regions of Asia, remains high enough. This article contains a summary of the available information about the spread of LSD in Asian part of Eurasia for the period of 1984 - February 2022. We are also discussing the latest available findings on the epidemiology of LSD and the methods currently used for the prevention and control of the LSDV.
</abstract>

Update of Lumpy Skin Disease: Emergence in Asian Part of Eurasia

<abstract><title style='display:none'>Abstract</title> The aim of this study was to compare the effects of different premedication protocols followed by a propofol/fentanyl TIVA on cardio-respiratory and hemodynamic changes in twenty-four dogs randomly divided into two groups (AMD-group: medetomidine, atropine and diazepam; AXD-group: xylazine, atropine and diazepam). Cardiorespiratory variables, acid-base indices, quality of sedation, induction, intubation and recovery were recorded throughout the experiment. Significant changes were observed for the pO2 level, which was increased in the AMDgroup from 90 min. (*P&lt; 0.05) to 120 min. (**P&lt; 0.01) of anesthesia. This can be explained by a reduction of the administration rate of propofol/fentanyl TIVA and oxygenation initiated due to excessively deep anesthesia detected by an anesthetsiologist, leading to improved ventilation and increased pO2. The pCO2 (*P &lt; 0.05) reached more preferable values during the first 30 min. and pH (**P&lt; 0.01) was significantly improved within the first 60 min. in the AXD-group thanks to less depressant effects of xylazine. Within the first 30 min. of anesthesia a significant heart rate difference between the groups was accompanied with significantly higher BP (hypertension) in the AXD-group (10 min. ***P&lt; 0.001, 30 min. **P&lt; 0.01). This points to the possibility of atropine application only in the case of a tendency to bradycardia followed by hypotension. It can be concluded that xylazine is a better option for the premedication of a propofol/ fentanyl TIVA in dogs undergoing a prolonged surgical intervention, in spite of the fact that lower sedation scores were attained. We have detected significantly less adverse cardio-respiratory and hemodynamic effects of xylazine, and a shorter recovery time when compared to medetomidine</abstract>

Small Animals Clinic, University of Veterinary Medicine and Pharmacy, Komenskeho 73, Kosice, Slovak Republic

Clinic for Ruminants, University of Veterinary Medicine and Pharmacy, Komenskeho 73, Kosice, Slovak Republic

Department of the environment, veterinary legislation and economy, University of Veterinary Medicine and Pharmacy, Komenskeho 73, Kosice, Slovak Republic

Cardiorespiratory and Hemodynamic Effects of Medetomidine or Xylazine with Atropine and Diazepam Premedication for Total Intravenous Anesthesia Induced and Maintained with Propofol/Fentanyl in Dogs Undergoing Surgery

<abstract><title style='display:none'>Abstract</title> The aim of this study was the investigation of water, sediments and fish tissues contamination with heavy metals. All samples were taken from the Danube River in Belgrade region, a location upstream from Batajnica. Concentrations of Cd, Hg and Pb in water samples were not detected, while concentrations of Zn, Fe, Cu and As were in the range of 0.004 - 0.330 mg L-1. Iron was the most deposited metal in sediment samples in contrast to water samples where all investigated metals were detected. For the purpose of heavy metals determination in fresh fish tissue, fifteen samples of three different fish species, silver carp (Hypophthalmichthys molitrix), common carp (Cyprinus carpio) and wels catfish (Silurus glanis) were collected. Concentrations of Pb, Cd, As and Hg were determined in the digestive tract, liver and muscle by absorption spectroscopy. The highest concentration of Pb was in the digestive tract in all three fish species, ranging from 0.036 to 1.518 μg g-1, while Cd was mostly deposited in the liver. Concentrations of As were in the range of 0.36 - 0.73 μg g-1 in Hypophthalmichthys molitrix, 0.013 - 0.18 μg g-1 in Cyprinus carpio and 0.003 - 0.005 μg g-1 in Silurus glanis tissues, while the content of Hg was equal in all tested tissues of carp. Concentrations of all metals were found to be present in the fish samples at different levels, but these values are under the maximum residual levels prescribed by the European Union (EU) and the maximum allowed concentrations (MAC) for Serbia, so the fish meat is acceptable for the human consumption.</abstract>

Ministry of Health Republic of Serbia, Omladinskih brigada 1, 11000 Belgrade, Serbia

Faculty of Veterinary Medicine, Bulevar Oslobodjenja 18, 11000 Belgrade, Serbia

Centre for Food Analysis, Zmaja od Noćaja 11, 11000 Belgrade, Serbia

Analysis of Heavy Metals Concentration in Tissues of Three Different Fish Species Included in Human Diet from Danube River

<abstract><title style='display:none'>Abstract</title>The aim of this study was to evaluate three combinations of antibiotics applied perorally prior to laparotomy and gastrointestinal surgery and to select the most favorable combination. Research was performed on 24 female and male dogs divided into four groups. Following abdominal wall incision the caecum was compressed manually and 5 mL of saline solution was injected into it. Five mL of caecum contents was aspirated in a sterile syringe and only 0.1 mL was incubated into blood agar and homogenous agar. Bacterial colonies were counted and determined. Prior to surgery the control group (n=6) received no antibiotics. The group which received the combination of gentamicin and clindamycin (n=6) achieved the best reduction of E. coli whereas Enterococcus faecals was 100% destroyed. The group which received amoxicillin with clavulanic acid and metronidazole (n=6) acquired a reduction of bacteria Enterococcus faecalis, which could be sufficient for prophylaxis. The combination of gentamicin and erythromycin (n=6) failed to accomplish a reduction of the number of bacteria.</abstract>

Veterinary practice, “Pets2Vets”, Koprivnica, Croatia;

Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia

Antimicrobial prophylaxis in addition to premedication in gastrointestinal surgery

<abstract>
<title style='display:none'>Abstract</title>
The aim of this study was to determine the length of the contraceptive period after the application of deslorelin implant (4.7 mg) in juvenile, prepubertal bitches and assess the effects on the hormonal status (P4, E2 and melatonin), as well as on the occurrence of normal physiological estrus. Twelve crossbred prepubertal female dogs (4 months of age) were divided into the two groups, the treated group (DESLO) and control (placebo) group (C). Hormone assays, vaginal microbiological and cytological smears and the measurements of electrical resistance of cervical mucus were performed once in 3 weeks until the first signs of proestrus, and every day during estrus. GnRH implant significantly postponed time of onset of estrus (633 ± 30,38 days vs. 143.80 ± 52,30 days, P&lt;0.001). In estrus, DESLO group showed statistically higher E2 (34.58 pg/mL vs. 20.59 pg/mL, P&lt;0.001) and melatonin serum concentration (45.86 pg/mL vs. 18.85 pg/mL, P&lt;0.001) compared to the control group. The average P4 serum concentration had no statistically significant differences between groups. In conclusion, deslorelin implants do not cause “flare up” effect in perpubertal bitches, however, increase of E2 and especially melatonin serum concentrations show the need to explore the consequences and mutual influence of melatonin and deslorelin implants in the future.
</abstract>

Clinical Use of Long-Term GnRH Agonist in Prepubertal Bitches: Effect on P4, E2 and Melatonin Serum Concentration before and During First Estrus

<abstract>
<title style='display:none'>Abstract</title>
Although the incidence of neoplasms increases with age, some tumors occur more commonly in younger dogs. Still, little information is available on the occurrence of neoplasms in dogs up to the age of 24 months. This study is a retrospective review of histopathological diagnoses of neoplasia at our laboratory during the last seven years (2017–2023) in two groups of dogs: group up to the age of 12 months and group from 13 to 24 months. In a total of 3704 neoplasms found in different dog tissues, 74 (2.00%) were identified in dogs up to 12 months and 135 (3.64%) in dogs aged 13 to 24 months. Canine cutaneous histiocytoma was the most frequent in both groups:41 cases (54.41%) in dogs up to 12 months, followed by 39 cases (28.89%) in those up to 24 months. In the present analysis of the group up to 12 months, 82.43% of tumors (n = 61) were benign, and 17.57% (n=13) were malignant, followed by 68.15% (n=92) benign neoplasms, and 31.85% (n=43) malignant in dogs aged from 13 to 24 months. French bulldogs and mixed-breed dogs were the most frequently affected. These data provide valuable epidemiological information on neoplasms in young dogs in the Republic of Serbia.
</abstract>

Seven-Year Follow-Up of Tumors in Young Dogs in the Republic of Serbia

<abstract>
<title style='display:none'>Abstract</title>
The effects of propylthiouracil (PTU) treatment and selenium-deficient diet on selenium and thyroid status of Wistar male rats were examined in this study. Wistar male rats (n =128) were divided into four groups: (1) control group – selenium-adequate rats fed a diet supplemented with 0.334 mg Na selenite/kg feed and received regular drinking water (Se+PTU-); (2) selenium adequate rats fed a diet supplemented with 0.334 mg Na selenite/kg feed and received a dose of 150 mg/L of PTU in drinking water, (Se+PTU+); (3) selenium-deficient rats fed a diet containing 0.031 mg Na selenite/ kg and received regular drinking water (Se-PTU-); (4) selenium deficient rats fed a diet containing 0.031 mg Na selenite/kg and received 150 mg/L of PTU in drinking water (Se-PTU+). After three and seven weeks of treatment, all Se – animals had significantly lower whole blood Se concentrations and GPx1 and GPx3 activities. PTU induced a significant decrease in T4 and T3 plasma concentrations after three weeks of treatment in both PTU+ groups. Furthermore, after seven weeks, the T3 level was close to its detection limit in Se – animals. A negative correlation was spotted between GPx activity and concentration of T3 after three and seven weeks. It could indicate an inhibitory influence of thyroid hormones on the expression and/or activities of GPx enzymes related to the available Se in conditions of systemic decrease of T4 concentration. This effect was particularly pronounced in Se – animals.
</abstract>

Effects of Combined Hypothyroidism and Selenium Deficiency on Selenoenzyme Glutathione Peroxidase Activity in Rats

<abstract>
<title style='display:none'>Abstract</title>
The aim of this study was to evaluate the importance of quantitative ultrasonography in the diagnosis of acute pancreatitis in dogs, as well as to examine the correlation between the results of ultrasound examination and the Vcheck cPL rapid test in dogs with suspected acute pancreatitis. A total of 37 dogs were included to the study – 15 healthy dogs and 22 dogs with gastrointestinal clinical signs which had at least 1 out of 3 criteria (vomiting, cranial abdominal pain and jaundice). Serum was collected to evaluate cPL level by Vcheck cPL rapid test. A score 0 implied the Vcheck cPL Rapid test result was less than 200 ng/ml. A score 1 implied Vcheck cPL Rapid test result between 200 and 400 ng/ml, and a score 2 more than 400 ng/ml. Ultrasound was performed in all dogs in order to investigate the pancreatic lesions within 48 hours and evaluate the level of pancreatitis by score from 0 to 2. The criteria for pancreatitis on ultrasonograms included enlargement or irregularity of the pancreas, hypoechoic areas of the pancreas and hyperechoic mesentery surrounding the pancreas. An ultrasound assessment of pancreatitis implied: score 0 when none of the criteria was present. Score 1 when 1–2 from 3 criteria were present, and score 2 when all of the 3 criteria were present. Correlation between Vcheck cPL Rapid test and pancreatic ultrasonography was examined. The results indicate that agreement of Vcheck cPL Rapid test and pancreatic ultrasonography had kappa values of 0.71 which are in the “moderate” agreement range. This means that both Vcheck cPL Rapid test and ultrasonography should be used in the diagnostics of acute pancreatitis in daily clinical practice. Additionally, quantitative ultrasound assessment can be helpful in distinguishing acute pancreatitis from normal pancreas.
</abstract>

Quantitative Pancreatic Ultrasonography and Vcheck cPL Rapid Test in Dogs with Suspected Acute Pancreatitis

<abstract>
<title style='display:none'>Abstract</title>
Genome-wide association study (GWAS) was used to detect candidate genes affecting economic traits in livestock. GWAS can detect single nucleotide polymorphisms (SNPs) in all chromosome regions. This study aimed to determine the genetic markers for body weight by GWAS in native cattle breeds of Indonesia. The Illumina Bovine 50K BeadChip was used to determine the candidate genes in three mixed-sex Indonesian cattle breeds of Bali (16 animals), Madura (16 animals), and Ongole grade (13 animals). All animals were raised at the Pasuruan Regency, East Java, Indonesia breeding station. The GWAS was performed in pooled sample of animals (45 animals) with the general linear model (GLM) method using SNP markers with minimum allele frequency (MAF) values more than 0.05 by TASSEL 5.0. software. Therefore, the body weight of cattle at 1 to 3 years of age was collected for each animal for computing Manhattan plot graphics. This research found that <italic>SUGT1</italic>, <italic>SF3A3</italic>, and <italic>DSCAM</italic> genes were detected as potential genetic markers for body weight in cattle breeds of Indonesia. The <italic>SUGT1</italic> and <italic>DSCAM</italic> genes were monomorphic in Bali cattle (<italic>Bos javanicus</italic>). In addition, both genes were significantly associated (<italic>P</italic>&lt;0.05) with the body weight of Ongole-grade cattle (<italic>Bos indicus</italic>) at three years of age. However, the <italic>SF3A3</italic> gene was significantly (<italic>P</italic>&lt;0.05) associated with body weight of Madura cattle (<italic>Bos indicus</italic>) at 2 and 3 years of age. In conclusion, the GWAS of pool animals reveals three candidate genes significantly associated with body weight in many cattle breeds of Indonesia. Further study to detect SNPs in candidate genes with sequencing method is essential to apply these findings practically.
</abstract>

Early Detection of Candidate Genes for Body Weight in Indonesian Cattle Breeds with Genome-Wide Association Study (GWAS)

<abstract>
<title style='display:none'>Abstract</title>
To clarify the changes in milk microbiota by lactation stage, i.e., d ays in milk (DIM) for Fresh of 0 to 21, Early (DIM of 22 to 80), Middle (DIM of 81 to 200), and Late (DIM 201 or more) lactation stages in dairy cows, we performed longitudinal (12 sampling at each stage, total 48 samples) and cross-sectional sampling (Fresh [n = 7], Early [n = 12], Middle [n = 11], and Late [n = 11] stages, total of 41 samples) to comprehensively analyze the microbiota in milk samples by 16S rRNA amplicon sequencing. Although the relative abundance of bacteria of the phylum Actinobacteria increased significantly in the Late lactation stage in the longitudinal study, no significant changes were observed in the cross-sectional study. While no changes were observed in the milk microbiota during the lactation stages, environmental factors appeared to have a comparatively larger impact than interindividual diversity on the composition of the milk microbiota. Furthermore, the findings illustrated the importance of selecting appropriate study designs to clarify changes in milk microbiota throughout the lactation period. The findings obtained in this study not only provide useful information for interpreting previous research results, but also provide knowledge that could be helpful when designing new studies.
</abstract>

Faculty of Veterinary Medicine, Okayama University of Science

Hiroshima University, Graduate School of Integrated Sciences for Life

Longitudinal and Cross-Sectional Studies to Evaluate Changes in Cow Milk Microbiota Over the Lactation Stages

<abstract><title style='display:none'>Abstract</title>Anthelmintics are some of the most widely used drugs in veterinary medicine. Here we review the mechanism of action of these compounds on nematode parasites. Included are the older classes of compounds; the benzimidazoles, cholinergic agonists and macrocyclic lactones. We also consider newer anthelmintics, including emodepside, derquantel and tribendimidine. In the absence of vaccines for most parasite species, control of nematode parasites will continue to rely on anthelmintic drugs. As a consequence, vigilance in detecting drug resistance in parasite populations is required. Since resistance development appears almost inevitable, there is a continued and pressing need to fully understand the mode of action of these compounds. It is also necessary to identify new drug targets and drugs for the continued effective control of nematode parasites.</abstract>

A brief review on the mode of action of antinematodal drugs

<abstract><title style='display:none'>Abstract</title>Colony losses, including those induced by the colony collapse disorder, are an urgent problem of contemporary apiculture which has been capturing the attention of both apiculturists and the research community. CCD is characterized by the absence of adult dead bees in the hive in which few workers and a queen remain, the ratio between the brood quantity and the number of workers is heavily disturbed in favor of the former, and more than enough food is present. Robbing behavior and pests usually attacking the weakened colony do not occur. In the present paper, the causes of the emergence of this problem are discussed, as well as the measures of its prevention.The following factors, which lead to colony losses, are analyzed: shortage of high-quality food (pollen and honey); infestation with parasites, primarily with <italic>Varroa destructor</italic>, and mixed virus infections; bacterial infections (American and European foulbrood), fungal infections (nosemosis and ascosphaerosis) and trypanosomal infections (lotmariosis); and, finally, general management of the apiary.Certain preventive measures are proposed: (1) providing ample high-quality forage and clean water, (2) avoiding sugarisation, i.e. superfluous use of sugar syrup, (3) meeting the nutritional needs of the colony, (4) when feeding bees, taking care of the timing and the composition of diet, avoiding pure sugar syrup which in excessive quantities may induce energetic and oxidative stress, (5) when there is a shortage of natural feed – honey in the brood chamber – use sugar syrup with natural/artificial supplements to avoid protein starvation, (6) organized control of <italic>V. destructor</italic> in the colonies is obligatory due to its vector role, and (7) compliance with hygienic and sanitary measures and principles of good apiculture practice and management in apiaries. To conclude, all preventive measures are feasible in compliance with rules and regulations concerning regular spring and autumn bee health monitoring by licensed veterinarians, who can propose adequate treatments if necessary.</abstract>

Looking for the causes of and solutions to the issue of honey bee colony losses

<abstract><title style='display:none'>Abstract</title>Canine babesiosis is a tick-borne disease caused by protozoal haemoparasites of different <italic>Babesia</italic> species. Babesiosis is one of the most important globally extended and quickly spreading tick-borne infections of dogs. This comprehensive review gives an in-depth overview of <italic>Babesia</italic> species currently identified in dogs together with relevant vector tick species and their geographical distribution, life cycle and transmission of parasite. The main mechanisms in the pathogenesis of babesiosis are described and elucidated by recent literature overview. As <italic>Babesia</italic> infection causes a disease with very variable clinical manifestations, special attention is given to clinical signs, laboratory features and clinicopathological findings. The diagnosis of canine babesiosis by microscopy, serological and molecular methods is reviewed, together with recent advances in mass spectrometry based assays. Accurate detection and species recognition are important for the selection of the appropriate therapy, monitoring and prediction of the outcome of the disease. Finally, guidelines for the treatment and prevention of canine babesiosis are given.</abstract>

Canine Babesiosis: Where Do We Stand?

<abstract><title style='display:none'>Abstract</title> Lumpy skin disease (LSD) is an important disease of cattle which is included in the OIE list of notifiable terrestrial animal diseases because of its great economic importance. The etiological agent is the Lumpy skin disease virus (LSDV). In the control of LSD attenuated strains of LSDV and SPPV are successfully used as vaccine strains in infected areas. In the case of vaccination policy, due to the possibility of mild or systemic post-vaccination reactions in vaccinated animals, the application of diagnostic procedures that will rapidly and specifically differentiate LSDV field strains from LSD vaccine virus strains are extremely important. Rapidity in diagnostics and disposal of infected animals is one of the key factors in the prevention of spreading the disease. In the presented study we have described the development and validation of two real-time TaqMan-PCR assays for a rapid, sensitive and specific detection of the virulent field LSDV strain currently circulating in the Balkan Peninsula. Specificity for the field strain and exclusivity for vaccine strains was tested on 171 samples from naturally infected and vaccinated animals. The results of this study show that both developed real-time PCR assays are more sensitive than the conventional nested PCR in detecting field LSDV strains thus enabling rapid and high-throughput detection of animals infected with field strains of LSDV. In conclusion, both KV-2 and FLI real-time PCR assays described in this study are simple, rapid, sensitive and suitable for routine use in a diagnostic laboratory and have the potential to replace conventional nested gel-based PCR assays as the standard procedure for the detection of field strains of LSDV in clinical samples.</abstract>

Department for Laboratory Diagnostic, Veterinary Specialized Institute „Kraljevo“, Kraljevo,

Virology Department, Scientific Veterinary Institute „Novi Sad“, Novi Sad,

Institute of Diagnostic Virology, Friedrich-Loeffler Institut, Greifswald-Insel Riems,

Real-Time PCR Assays for the Specific Detection of Field Balkan Strains of Lumpy Skin Disease Virus

<abstract><title style='display:none'>Abstract</title> Canine monocytic ehrlichiosis (CME) is a tick-borne disease of worldwide distribution. The major causative agent is Ehrlichia canis, a gram-negative, obligate intracellular, pleomorphic bacterium of the genus Ehrlichia, which infects monocytes, macrophages and lymphocytes, forming intracytoplasmic, membrane-bound bacterial aggregates, called morulae. After an incubation period of 8-20 days, the course of E. canis infection, can be sequentially divided into acute, subclinical and chronic phases, although these phases can hardly be distinguished in the clinical setting. Clinical recovery is the typical outcome of acutely infected dogs, entering the subclinical phase, during which they show no or minimal clinical signs and/or mild hematological abnormalities. Immunocompetent dogs may eliminate the infection during the acute or subclinical phases, but an unpredictable proportion of dogs will eventually develop the chronic phase, characterized by aplastic pancytopenia and high mortality, due to septicemia and/or severe bleeding. This article outlines briefly the pathogenesis of CME due to E. canis, and more thoroughly reviews the recent scientific literature pertaining to the diagnosis and treatment of this devastating disease.</abstract>

Canine Monocytic Ehrlichiosis: An Update on Diagnosis and Treatment

<abstract><title style='display:none'>Abstract</title>Infection of cattle with lumpy skin disease virus (LSDV) is very important from the aspect of livestock production. Although it can cause significant economic losses, available serological assays are still not sufficiently efficient and reliable. A 3-day VNT was performed using Madin-Darby bovine kidney (MDBK) cell line and LSDV isolated from clinically infected cow to improve serological diagnostics of lumpy skin disease (LSD).In total, 325 cattle sera samples were tested in order to compare the performances of VNT and ELISA. Tested samples originated from 125 cows before the occurrence of LSD in the Republic of Serbia and 200 tested samples originated from vaccinated cows. Sera samples from vaccinated cows were collected starting from the vaccination day to 4 months after vaccination. In 7 different time intervals after vaccination sampling was carried out in 20 cows originating from one herd and in 3 different time intervals in 20 cows originating from a different herd each time of sampling.Out of 200 samples from vaccinated cows, antibodies against LSDV were detected in 68 (34%) samples by VNT, and in 60 (30%) samples by ELISA. No positive finding was detected by VNT in samples collected before the occurrence of LSD in Serbia, while one positive finding was detected in the same samples by ELISA. The first presence of antibodies in vaccinated cattle was detected by both tests 20 days after vaccination, and the largest number of animals with antibodies against LSDV was detected 30 days after vaccination.Comparing the results obtained by VNT and ELISA, it was calculated that <italic>kappa</italic> index was 0.913. The specificity of VNT and ELISA was 100% and 99.2%, respectively. VNT is simpler to perform compared to the recommended virus neutralization test by the OIE and can improve LSD serological diagnostics with additional sensitivity testing.</abstract>

Detection of antibodies against Lumpy skin disease virus by Virus neutralization test and ELISA methods

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Acta Veterinaria

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Acta Veterinaria is an open access, peer-reviewed scientific journal of the Faculty of Veterinary Medicine, University of Belgrade, Serbia, dedicated to the publication of original research...

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Acta Veterinaria is an open access, peer-reviewed scientific journal of the Faculty of Veterinary Medicine, University of Belgrade, Serbia, dedicated to the...