Inhibitory effect of dimethyl sulfoxide on the development of gastrointestinal nematode larvae in the larval development test
Marcin Mickiewicz
Profil Orcid, , Michał Czopowicz
Profil Orcid, Agata Moroz-Fik
Profil Orcid, Adrian-Valentin Potărniche
Profil Orcid, Kinga Biernacka
Profil Orcid, Olga Szaluś-Jordanow
Profil Orcid, Paweł Górski
Profil Orcid, , Iwona Markowska-Daniel
Profil Orcid, et 
Publié en ligne: 25 mars 2025
Pages: 83 - 90
Reçu: 12 sept. 2024
Accepté: 11 mars 2025
DOI: https://doi.org/10.2478/jvetres-2025-0016
Mots clés
© 2025 Marcin Mickiewicz et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.
Introduction
Dimethyl sulfoxide (DMSO) is an amphipathic solvent for molecules in
Material and Methods
A standard LDT was performed in 30 replications at DMSO concentrations of 0.0% (control), 0.6%, 1.3%, 2.6%, 5.2%, 10.4%, and 20.8%. The numbers of all developmental stages of
Results
The PD significantly decreased with increasing DMSO concentration and was significantly reduced at the 2.6% concentration. The median inhibitory concentration (IC50) was 3.79%, the concentration for 10% inhibition (IC10) was 1.75% and for 90% inhibition (IC90) was 8.20%. The percentage of L1 and L2 followed an analogical but opposite pattern to that of PD and was complementary to it at each DMSO concentration. The unhatched egg percentage was rarely >1% and showed no pattern.
Conclusion
At ≥2.6% concentration, DMSO significantly inhibited the L3 development of all three GIN species. It had a practically important inhibitory effect (IC10) at as low concentration as 1.75%. At lower concentrations, DMSO did not appear to inhibit larval development. The compound did not seem to exert an