Inhibitory effect of dimethyl sulfoxide on the development of gastrointestinal nematode larvae in the larval development test
Marcin Mickiewicz
Perfil de Orcid, , Michał Czopowicz
Perfil de Orcid, , Adrian-Valentin Potărniche
Perfil de Orcid, , Olga Szaluś-Jordanow
Perfil de Orcid, Paweł Górski
Perfil de Orcid, , Iwona Markowska-Daniel
Perfil de Orcid, y 
Publicado en línea: 25 mar 2025
Páginas: 83 - 90
Recibido: 12 sept 2024
Aceptado: 11 mar 2025
DOI: https://doi.org/10.2478/jvetres-2025-0016
Palabras clave
© 2025 Marcin Mickiewicz et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.
Introduction
Dimethyl sulfoxide (DMSO) is an amphipathic solvent for molecules in
Material and Methods
A standard LDT was performed in 30 replications at DMSO concentrations of 0.0% (control), 0.6%, 1.3%, 2.6%, 5.2%, 10.4%, and 20.8%. The numbers of all developmental stages of
Results
The PD significantly decreased with increasing DMSO concentration and was significantly reduced at the 2.6% concentration. The median inhibitory concentration (IC50) was 3.79%, the concentration for 10% inhibition (IC10) was 1.75% and for 90% inhibition (IC90) was 8.20%. The percentage of L1 and L2 followed an analogical but opposite pattern to that of PD and was complementary to it at each DMSO concentration. The unhatched egg percentage was rarely >1% and showed no pattern.
Conclusion
At ≥2.6% concentration, DMSO significantly inhibited the L3 development of all three GIN species. It had a practically important inhibitory effect (IC10) at as low concentration as 1.75%. At lower concentrations, DMSO did not appear to inhibit larval development. The compound did not seem to exert an