1. bookVolume 21 (2013): Issue 1 (March 2013)
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Journal
eISSN
2284-5623
ISSN
2284-5623
First Published
08 Aug 2013
Publication timeframe
4 times per year
Languages
English
access type Open Access

Inhibition of Streptococcus pneumoniae adenylate kinase by some 5-arylidene-thiazolidin-4-on-2-thione derivates

Journal Details
License
Format
Journal
eISSN
2284-5623
ISSN
2284-5623
First Published
08 Aug 2013
Publication timeframe
4 times per year
Languages
English
Abstract

Adenylat kinase is an ubiquitous enzyme found in prokaryotes and eukaryotes. In the present study we examined the inhibition of Streptococcus pneumoniae adenylate kinase (AKSP) by six 5-arylidene-thiazolidin-4- on-2-thione derivates using 2, 4-dinitrophenylhydrazine colorimetric assay. Inhibition of AKSP activity with synthetic compounds was performed against recombinant enzyme over-expressed in E. coli. The compound C10H6BrNOS2 with the bromine atom in -ortho position has shown the most efficient inhibitory activity; I50 value (the inhibitor concentration that leads to 50% activity inhibition) was 0.067mM.

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Cuvinte cheie

1. Oakhill JS, Scott JW, Kemp BE. AMPK functions as an adenylate charge-regulated protein kinase. Trends Endocrinol Metab. 2012; 23(3):125-3210.1016/j.tem.2011.12.006Search in Google Scholar

2. Daily MD, Phillips GN Jr, Cui Q. Interconversion of functional motions between mesophilic and thermophilic adenylate kinases. PLoS Comput Biol. 2011; 7(7):e1002103. Epub 2011 Jul 14Search in Google Scholar

3. Lehmann-Munier H, Chenal-Francisque V, Ionescu M, Christova P, Foulon J, Carniel E, et al. Relationship between bacterial virulence and nucleotide metabolism: a mutation in the adenylate kinase gene renders Yersinia pestis avirulent. Biochem J. 2003; 373(2): 515-2210.1042/bj20030284Search in Google Scholar

4. Saint-Girons I, Gilles AM, Margarita D, Michelson S, Monnot M, Fermandjian S, et al. Structural and catalytic 98 characteristics of Escherichia coli adenylate kinase. J. Biol. Chem. 1987; 262(2): 622-9Search in Google Scholar

5. Tuck KL, Saldanha SA, Birch LM, Smith AG, Abell C. The design and synthesis of inhibitors of pantothenate synthetase. Org Biomol Chem. 2006; 4(19):3598-61010.1039/b609482aSearch in Google Scholar

6. Buchko GW, Robinson H, Abendroth J, Staker BL, Myler PJ. Structural characterization of Burkholderia pseudomallei adenylate kinase (Adk): profound asymmetry in the crystal structure of the 'open' state. Biochem Biophys Res Commun. 2010; 394(4):1012-710.1016/j.bbrc.2010.03.112Search in Google Scholar

7. Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, et al. Gapped BLAST and PSIBLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997; 25(17): 3389-40210.1093/nar/25.17.3389Search in Google Scholar

8. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positionspecific gap penalties and weight matrix choice. Nucleic Acids Res. 1994; 22: 4673-8010.1093/nar/22.22.4673Search in Google Scholar

9. Crowe J, Dobeli H, Gentz R, Hochuli E, Stuber D, Henco K. 6xHis-Ni-NTA chromatography as a superior technique in recombinant protein expression/purification. Methods Mol. Biol. 1994; 31:371-87Search in Google Scholar

10. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72: 248-5410.1016/0003-2697(76)90527-3Search in Google Scholar

11. Laemmli UK Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227(5259): 680-68510.1038/227680a05432063Search in Google Scholar

12. Oniga O, Moldovan C, Muntean A, Verite P, Ionescu M, Tiperciuc B. Synthesis and antibacterial activity of some 5-aryliden-2-thioxo-thiazolydin-4-one. Revista de Medicina si Farmacie 2004; 50, Supl.II: 183-186Search in Google Scholar

13. Ionescu M, Radulescu AZ, Moldovan C, Banciu HL, Ghiran D. Inhibition of E.coli adenylate kinase by some 5- aryliden-2-thioxo-thiazolydin-4-one derivatives. Clujul Medical 2005; 3:638-41.Search in Google Scholar

14. Grochowski LL, Censky K, Xu H, White RH. A new class of adenylate kinase in methanogens is related to uridylate kinase. Arch Microbiol. 2012;194(2):141-5. Epub 2011 Oct 15.10.1007/s00203-011-0759-922002406Search in Google Scholar

15. Bae E, Phillips GN Jr. Structures and analysis of highly homologous psychrophilic, mesophilic, and thermophilic adenylate kinases. J.Biol Chem. 2004; 279(27):28202-810.1074/jbc.M40186520015100224Search in Google Scholar

16. Vicini P, Geronikaki A, Anastasia K, Incerti M, Zani F. Synthesis and antimicrobial activity of novel 2- thiazolylimino-5-arylidene-4-thiazolidinones. Bioorg Med Chem. 2006;14(11):3859-6410.1016/j.bmc.2006.01.04316488614Search in Google Scholar

17. Segall-Shapiro TH, Nguyen PQ, Dos Santos ED, Subedi S, Judd J, Suh J, et al. Mesophilic and hyperthermophilic adenylate kinases differ in their tolerance to random fragmentation. J Mol Biol. 2011; 406(1):135-48.10.1016/j.jmb.2010.11.05721145325Search in Google Scholar

18. Gilles AM, Glaser P, Perrier V, Meier A, Longin R, Sebald M, et al. Zinc, a structural component of adenylate kinases from gram-positive bacteria. J Bacteriol. 1994; 176(2):520-3.10.1128/jb.176.2.520-523.19942050788288548Search in Google Scholar

19. Gresh N. Development, validation, and applications of anisotropic polarizable molecular mechanics to study ligand and drug-receptor interactions. Curr Pharm Des. 2006; 12(17):2121-58.10.2174/13816120677758525616796560Search in Google Scholar

20. Kistler KA, Matsika S. The fluorescence mechanism of 5-methyl-2-pyrimidinone: an ab initio study of a fluorescent pyrimidine analog. Photochem Photobiol. 2007; 83(3):611-24.Search in Google Scholar

21. Seclaman E, Bora A, Avram S, Simon Z, Kurunczi L. MTD-PLS and docking study for a series of substituted 2-phenylindole derivatives with oestrogenic activity. Chemical Papers. 2011; 65 (4): 566-576.10.2478/s11696-011-0040-3Search in Google Scholar

22. Miura K, Inouye S, Sakai K, Takaoka H, Kishi F, Tabuchi M, et al. Cloning and characterization of adenylate kinase from Chlamydia pneumoniae. J Biol Chem. 2001; 276(16):13490-8.10.1074/jbc.M00946120011278507Search in Google Scholar

23. Oakhill JS, Steel R, Chen ZP, Scott JW, Ling N, Tam S, et al. AMPK is a direct adenylate charge-regulated protein kinase. Science. 2011; 332(6036):1433-5. 10.1126/science.120009421680840Search in Google Scholar

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