1. bookVolume 24 (2016): Issue 3 (September 2016)
Journal Details
License
Format
Journal
eISSN
2284-5623
First Published
08 Aug 2013
Publication timeframe
4 times per year
Languages
English
access type Open Access

Malondialdehyde levels can be measured in serum and saliva by using a fast HPLC method with visible detection / Determinarea printr-o metodă HPLC-VIS rapidă a concentraţiilor serice şi salivare ale malondialdehidei

Published Online: 15 Oct 2016
Page range: 319 - 326
Received: 19 May 2016
Accepted: 17 Aug 2016
Journal Details
License
Format
Journal
eISSN
2284-5623
First Published
08 Aug 2013
Publication timeframe
4 times per year
Languages
English
Abstract

Oxidative stress appears when the amount of free radicals that are formed in a living organism exceed its spin-trapping ability. One of the most dangerous free radicals that are formed in the human body is the hydroxyl radical. It can alter several biomolecules, including the unsaturated fatty acids; this process is known as lipid peroxidation and can lead to cell necrosis and generation of several harmful byproducts including malondialdehyde, which serves also as a biomarker of oxidative stress. A new HPLC method with visible detection was developed for the detection of malondialdehyde in human serum and saliva samples. The method was verified in terms of specificity, linearity, limits of detection (0.35 ng/ml), limit of quantification (1.19 ng/ml), recovery (90.13±10.25 – 107.29±14.33) and precision (3.84±1.49% – 6.66±1.76%). An analysis time of only 1 minute was obtained and no interferences from the matrices were observed. Statistical analysis (Pearson correlation test) showed a moderate correlation (R = 0.5061, p = 0.0099) between serum and saliva concentrations (N = 25). The possibility of measuring salivary concentrations of malondialdehyde extents the applications of oxidative stress/lipid peroxidation estimations to categories of population unreachable before (pregnant women, small children, etc); repeated sample studies are also easier to make.

Keywords

1. Chen SX, Schopfer P. Hydroxyl-radical production in physiological reactions. A novel function of peroxidase. Eur J Biochem. 1999 Mar;260:726-735. DOI: 10.1046/j.1432-1327.1999.00199.x.10.1046/j.1432-1327.1999.00199.x10103001Search in Google Scholar

2. Lipinski B. Hydroxyl radical and its scavengers in health and disease. Oxid Med Cell Longev. 2011 Iun;2011:809696.10.1155/2011/809696316678421904647Search in Google Scholar

3. Gregus Zoltán, Mechanisms of toxicity, Casarett & Doull’s Toxicology. The basic science of poisons, McGraw-Hill Companies Kansas, 2008, 45-106.Search in Google Scholar

4. Ansley DM, Wang B. Oxidative stress and myocardial injury in the diabetic heart. J. Pathol. 2013 Jan;229(2):232-241. DOI: 10.1002/path.4113.10.1002/path.4113362056723011912Search in Google Scholar

5. Gulcin İ. Antioxidant activity of food constituents: an overview. Arch Toxicol. 2012 Mar;86(3):345-391. DOI: 10.1007/s00204-011-0774-2.10.1007/s00204-011-0774-222102161Search in Google Scholar

6. Micallef M, Lexis L, Lewandowski P. Red wine consumption increases antioxidant status and decreases oxidative stress in the circulation of both young and old humans. Nutr J. 2007 Sep;6:27. DOI: 10.1186/1475-2891-6-27.10.1186/1475-2891-6-27203972917888186Search in Google Scholar

7. Dalle-Donne I, Rossi R, Colombo R, Giustarini D, Milzani A. Biomarkers of oxidative damage in human disease. Clin Chem. 2006 Apr;52(4):601-23. DOI: 10.1373/clinchem.2005.061408.10.1373/clinchem.2005.06140816484333Search in Google Scholar

8. Sandor R, Leucuta D, Dronca E, Niculae A, Cret V, Silaghi C, et al. Low Serum Paraoxonase-1 Lactonase and Arylesterase Activities in Obese Children and Adolescents. Rev Romana Med Lab. 2015 Dec;23(4):385-396. DOI: 10.1515/rrlm-2015-0038.10.1515/rrlm-2015-0038Search in Google Scholar

9. Lefevre G, Bonneau C, Rahma S, Chanu B, Brault D, Couderc R, et al. Determination of plasma protein- bound malondialdehyde by derivative spectrophotometry. Eur J Clin Chem Clin Biochem. 1996 Aug;34(8):631-6. DOI: 10.1515/cclm.1996.34.8.631.10.1515/cclm.1996.34.8.6318877339Search in Google Scholar

10. Lovric J, Mesic M, Macan M, Koprivanac M, Kelava M, Bradamante V. Measurement of malondialdehyde (MDA) level in rat plasma after simvastatin treatment using two different analytical methods. Period. Biol. 2008 Feb;110(1):63-67.Search in Google Scholar

11. Hosen MB, Islam MR, Begum F, Kabir Y, Howlader MZ. Oxidative stress induced sperm DNA damage, a possible reason for male infertility. Iran J Reprod Med. 2015 Sep;13(9):525-32.Search in Google Scholar

12. Al-Said MS, Mothana RA, Al-Yahya MM, Rafatullah S, Al-Sohaibani MO, Khaled JM, et al. GC-MS Analysis: In Vivo Hepatoprotective and Antioxidant Activities of the Essential Oil of Achillea biebersteinii Afan. Growing in Saudi Arabia. Evid Based Complement Alternat Med. 2016;2016:1867048.Search in Google Scholar

13. Sadeghi H, Hosseinzadeh S, Akbartabar Touri M, Ghavamzadeh M, Jafari Barmak M, Sayahi M, Sadeghi H. Hepatoprotective effect of Rosa canina fruit extract against carbon tetrachloride induced hepatotoxicity in rat. Avicenna J Phytomed. 2016 Mar-Apr;6(2):181-8.Search in Google Scholar

14. Ali F, Naqvi SA, Bismillah M, Wajid N. Comparative analysis of biochemical parameters in diabetic and non-diabetic acute myocardial infarction patients. Indian Heart J. 2016 May-Jun;68(3):325-31. DOI: 10.1016/j.ihj.2015.09.026.10.1016/j.ihj.2015.09.026491144927316485Search in Google Scholar

15. Liu X, Liu M, Mo Y, Peng H, Gong J, Li Z, Chen J, Xie J. Naringin ameliorates cognitive deficits in streptozotocin- induced diabetic rats. Iran J Basic Med Sci. 2016 Apr;19(4):417-22.10.1007/s11011-015-9779-526686502Search in Google Scholar

16. Tian XH, Liu CL, Jiang HL, Zhang Y, Han JC, Liu J, Chen M. Cardioprotection provided by Echinatin against ischemia/reperfusion in isolated rat hearts. BMC Cardiovasc Disord. 2016 May 31;16:119. DOI: 10.1186/s12872-016-0294-3.10.1186/s12872-016-0294-3Search in Google Scholar

17. Iliesiu A, Campeanu A, Marta D, Parvu I, Gheorghe G. Uric Acid, Oxidative Stress and Inflammation in Chronic Heart Failure with Reduced Ejection Fraction. Rev Romana Med Lab. 2015 Dec;23(4):397-406. DOI: 10.1515/rrlm-2015-0039.10.1515/rrlm-2015-0039Search in Google Scholar

18. Wasowicz W, Nève J, Peretz A. Optimized steps in fluorometric determination of thiobarbituric acid-reactive substances in serum: importance of extraction pH and influence of sample preservation and storage. Clin Chem. 1993 Dec;39(12):2522-6.10.1093/clinchem/39.12.2522Search in Google Scholar

19. Grotto D, Lucas SM, Valentini J, Paniz C, Schmitt G, Garcia SC. Importance of the lipid peroxidation biomarkers and methodological aspects for malondialdehyde quantification. Quim. Nova 2009;32(1):169-74. DOI: 10.1590/S0100-40422009000100032.10.1590/S0100-40422009000100032Search in Google Scholar

20. Hong YL, Yeh SL, Chang CY, Hu ML. Total plasma malondialdehyde levels in 16 Taiwanese college students determined by various thiobarbituric acid tests and an improved high-performance liquid chromatography- based method. Clin Biochem. 2000 Nov;33(8):619-25. DOI: 10.1016/S0009-9120(00)00177-6.10.1016/S0009-9120(00)00177-6Search in Google Scholar

21. Tsaknis J, Lalasa S, Holeb M, Smithb G, Tychopoulosa V. Rapid high-performance liquid chromatographic method of determining malondialdehyde for evaluation of rancidity in edible oils. Analyst 1998 Feb.;123:325-27. DOI: 10.1039/a706812c.10.1039/a706812cSearch in Google Scholar

22. Karatas F, Karatepe M, Baysar A. Determination of free malondialdehyde in human serum by high-performance liquid chromatography. Anal Biochem. 2002 Dec.;311(1):76-9. DOI: 10.1016/S0003-2697(02)00387-1.10.1016/S0003-2697(02)00387-1Search in Google Scholar

23. Babaee N, Hosseinkazemi H, Pouramir M, Khakbaz Baboli O, Salehi M, Khadir F, et al. Salivary oxidant/ antioxidant status and hematological parameters in patients with recurrent aphthous stomatitis. Caspian J Intern Med. 2016 Winter;7(1):13-8.Search in Google Scholar

24. Smriti K, Pai KM, Ravindranath V, Pentapati KC. Role of salivary malondialdehyde in assessment of oxidative stress among diabetics. J Oral Biol Craniofac Res. 2016 Jan-Apr;6(1):41-4. DOI: 10.1016/j.jobcr.2015.12.004.10.1016/j.jobcr.2015.12.004475606726937368Search in Google Scholar

25. Tsaknis J, Lalas S, Evmorfopoulos E. Determination of malondialdehyde in traditional fish products by HPLC. Analyst. 1999 Jun;124(6):843-5. DOI: 10.1039/ a902026h.10.1039/a902026h10736869Search in Google Scholar

26. Siddique YH, Ara G, Afzal M. Estimation of lipid peroxidation induced by hydrogen peroxide in cultured human lymphocytes. Dose Response. 2012;10(1):1-10. DOI: 10.2203/dose-response.10-002.Siddique.10.2203/dose-response.10-002.Siddique329952422423225Search in Google Scholar

27. Khalili J, Biloklytska HF. Salivary malondialdehyde levels in clinically healthy and periodontal diseased individuals. Oral Dis. 2008 Nov;14(8):754-60. DOI: 10.1111/j.1601-0825.2008.01464.x.10.1111/j.1601-0825.2008.01464.x18665859Search in Google Scholar

28. Tüközkan AN, Erdamar H, Seven I. Measurement of Total Malondialdehyde in Plasma and Tissues by High- Performance Liquid Chromatography and thiobarbituric acid assay, Fırat Tıp Dergisi 2006;11(2): 88-92.Search in Google Scholar

29. Lykkesfeldt J. Determination of malondialdehyde as dithiobarbituric acid adduct in biological samples by HPLC with fluorescence detection: comparison with ultraviolet-visible spectrophotometry. Clin Chem. 2001 Sep;47(9):1725-7.10.1093/clinchem/47.9.1725Search in Google Scholar

30. Mendes R, Cardoso C, Pestana C. Measurement of malondialdehyde in fish: A comparison study between HPLC methods and the traditional spectrophotometric test. Food Chem. 2009 Feb, 112(4):1038-45. DOI: 10.1016/j.foodchem.2008.06.052.10.1016/j.foodchem.2008.06.052Search in Google Scholar

31. Akalin FA, Baltacioğlu E, Alver A, Karabulut E. Lipid peroxidation levels and total oxidant status in serum, saliva and gingival crevicular fluid in patients with chronic periodontitis. J Clin Periodontol. 2007 Jul;34(7):558-65. DOI: 10.1111/j.1600-051X.2007.01091.x. 10.1111/j.1600-051X.2007.01091.x17555410Search in Google Scholar

Recommended articles from Trend MD

Plan your remote conference with Sciendo