1. bookVolume 24 (2016): Issue 1 (March 2016)
Journal Details
License
Format
Journal
eISSN
2284-5623
First Published
08 Aug 2013
Publication timeframe
4 times per year
Languages
English
access type Open Access

FGB -455 G>A and GP IIIa PIA1/A2 polymorphisms in a group of Romanian stroke patients

Published Online: 19 Mar 2016
Page range: 45 - 54
Received: 11 Aug 2015
Accepted: 16 Dec 2015
Journal Details
License
Format
Journal
eISSN
2284-5623
First Published
08 Aug 2013
Publication timeframe
4 times per year
Languages
English
Abstract

Introduction: Being a multifactorial disease, stroke is one of a major causes of death and disability worldwide. Several genetic polymorphisms have been associated with stroke etiophatology and FGB −455 G>A and GP IIIa PIA1/A2 are among them. In the present study, we investigated the association between FGB −455 G>A and GP IIIa PIA1A2 polymorphisms and the risk of ischemic stroke in a group of Romanian stroke patients.

Subjects and methods: This case-control study included 148 patients with ischemic stroke and 150 healthy age, sex and ethnically matched unrelated controls. FGB −455G>A and GP IIIa PIA1A2 genotyping was carried out using PCR-RFLP. The association of FGB −455G>A and GP IIIa PIA1A2 polymorphisms and cardiovascular risk factors with ischemic stroke was tested using logistic regression analysis.

Results: Molecular analysis did not reveal an increased frequency of the FGB -455 G>A variant allele and GP IIIa PIA1/A2 variant allele in the study group compared to the control group (p = 0.140, OR = 0.750, 95% CI = 0.522 - 1.077; p = 0.823, OR = 0.944, 95% CI = 0.558 - 1.599 respectively). Furthermore, after performing logistic regression analysis adjusted for the known risk factors, a positive association with stroke was found in smokers (p = 0.026, OR = 1.800, 95% CI = 1.071 - 3.024)

Conclusions: No association was found between FGB −455 G>A and GP IIIa PIA1/A2 polymorphisms and ischemic stroke in the studied population.

Keywords

1. Ohman EM, Bhatt DL, Steg PG, Goto S, Hirsch AT, Liau CS, et al. The Reduction of Atherothrombosis for Continued Health (REACH) Registry: an international, prospective, observational investigation in subjects at risk for atherothrombotic events-study design. Am Heart J. 2006 Apr;151(4):786-96. DOI: 10.1016/j.ahj.2005.11.00410.1016/j.ahj.2005.11.00416569533Search in Google Scholar

2. Markus HS. Stroke genetics. Hum Mol Genet. 2011 Oct 15;20(R2):R124-31. DOI: 10.1093/hmg/ddr34510.1093/hmg/ddr34521831884Search in Google Scholar

3. Cinteza M, Pana B, Cochino M, Florescu M, Margulescu M, Florian A, et al. Prevalence and control of cardiovascular risk factors in Romania cardio-zone national study. Mædica. 2007 May; 2(4):277-88.Search in Google Scholar

4. Della-Morte D, Guadagni F, Palmirotta R, Testa G, Caso V, Paciaroni M, et al. Genetics of ischemic stroke, stroke-related risk factors, stroke precursors and treatments. Pharmacogenomics. 2012 Apr;13(5):595-613. DOI: 10.2217/pgs.12.1410.2217/pgs.12.1422462751Search in Google Scholar

5. Goracy J, Goracy I, Kaczmanczyk M, Brykcz M, Widwcka-Ostreska K, Taryna O, et al. Common genetic polymorphisms and environmental risk factors in Polish patients with angiographically documented coronary artery disease. Pol.J.Environ. 2011 May;20(6):1491-99.Search in Google Scholar

6. Sheweita SA, Baghdadi H, Allam AR. Role of genetic changes in the progression of cardiovascular diseases. Int J Biomed Sci. 2011 Dec;7(4):238-48.Search in Google Scholar

7. Imran Imran, Rusdi Lamsudin, Ponpon Idjiradinata, Tri Hanggono Achmad, Amelani Maskoen, Samekto Wibowo, et al. Association of β-fibrinogen promoter gene polymorphism (-148C/T), hyperfibrinogenemia and ischemic stroke in young adult patients. Egypt J Med Hum Genet. 2015 Jan;16(1):11-17. DOI: 10.1016/j.ejmhg.2014.09.00410.1016/j.ejmhg.2014.09.004Search in Google Scholar

8. Emerging Risk Factors Collaboration, Kaptoge S, Di Angelantonio E, Pennells L, Wood AM, White IR, Gao P, et al. C-reactive protein, fibrinogen, and cardiovascular disease prediction. N Engl J Med. 2012 Oct 4;367(14):1310-20. DOI: 10.1056/NEJMoa110747710.1056/NEJMoa1107477371410123034020Search in Google Scholar

9. Bentley P, Peck G, Smeeth L, Whittaker J, Sharma P. Causal relationship of susceptibility genes to ischemic stroke: comparison to ischemic heart disease and biochemical determinants. PLoS One. 2010 Feb 9;5(2):9136. DOI: 10.1371/journal.pone.000913610.1371/journal.pone.0009136281772620161734Search in Google Scholar

10. Cao YJ, Zhang X, Wang WH, Zhai WQ, Qian JF, Wang JS. Oral fibrinogen-depleting agent lumbrokinase for secondary ischemic stroke prevention: results from a multicenter, randomized, parallel-group and controlled clinical trial. Chin Med J (Engl). 2013 Nov;126(21):4060-5.Search in Google Scholar

11. Kumar A, Sagar R, Kumar P, Sahu JK, Grover A, Srivastava AK, et al. Identification of genetic contribution to ischemic stroke by screening of single nucleotide polymorphisms in stroke patients by using a case control study design. BMC Neurol. 2013 Oct 3;13:136. DOI: 10.1186/1471-2377-13-13610.1186/1471-2377-13-136385155824090469Search in Google Scholar

12. Martiskainen M, Pohjasvaara T, Mikkelsson J, Mäntylä R, Kunnas T, Laippala P, et al. Fibrinogen gene promoter -455 A allele as a risk factor for lacunar stroke. Stroke. 2003 Apr;34(4):886-91. DOI: 10.1161/01.STR.0000060029.23872.5510.1161/01.STR.0000060029.23872.5512637691Search in Google Scholar

13. Wu G, Cai H, Cai H, Chen Z, Tan L, Qi H, et al. Effect of the -148C/T, 448G/A, and -854G/A Polymorphisms of the β-Fibrinogen Gene on the Risk of Ischemic Stroke in Chinese Population. J Stroke Cerebrovasc Dis. 2015 Jul;24(7):1577-90. DOI: 10.1016/j.jstrokecerebrovasdis.2015.03.02910.1016/j.jstrokecerebrovasdis.2015.03.02925890854Search in Google Scholar

14. Pongrácz E, Andrikovics H, Csornai M, Bernát IS, Nagy Z. Contribution of the -455G/A polymorphism at beta-fibrinogen gene and of the Leiden mutation to hemorheological parameters in ischemic stroke patients. Clin Hemorheol Microcirc. 2006;35(1-2):75-82.Search in Google Scholar

15. Floyd CN, Ferro A. The platelet fibrinogen receptor: from megakaryocyte to the mortuary. JRSM Cardiovasc Dis. 2012 May 31;1(2). pii: cvd.2012.012007.10.1258/cvd.2012.012007373832424175064Search in Google Scholar

16. Galasso G, Santulli G, Piscione F, De Rosa R, Trimarco V, Piccolo R, et al. The GPIIIA PlA2 polymorphism is associated with an increased risk of cardiovascular adverse events. BMC Cardiovasc Disord. 2010 Sep 16;10:41. DOI: 10.1186/1471-2261-10-4110.1186/1471-2261-10-41295487420846430Search in Google Scholar

17. Floyd CN, Ellis BH, Ferro A. The PlA1/A2 polymorphism of glycoprotein IIIa as a risk factor for stroke: a systematic review and meta-analysis. PLoS One. 2014 Jul 2;9(7):e100239. DOI: 10.1371/journal.pone.0100239 DOI: 10.1371/journal.pone.010151810.1371/journal.pone.0100239407924524988537Search in Google Scholar

18. Lievens D, von Hundelshausen P. Platelets in atherosclerosis. Thromb Haemost. 2011 Nov;106(5):827-38. DOI: 10.1160/TH11-08-059210.1160/TH11-08-059222012554Search in Google Scholar

19. Nurden AT. Platelets, inflammation and tissue regeneration. Thromb Haemost. 2011May;105 Suppl 1:S13-33. DOI: 10.1160/THS10-11-072010.1160/THS10-11-072021479340Search in Google Scholar

20. Promega Corporation, Instructions for Use of Product(s) A1120, A1125, A1620, Wiyard Genomic DNA Purification Kit Technical Manual, Ma, USA, revised 12/2010. www.promega.com.Search in Google Scholar

21. Available from: http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=5918. [cited 2015.10.30].Search in Google Scholar

22. Available from: http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=1800790. [cited 2015.10.30].Search in Google Scholar

23. Undas A. Fibrin clot properties and their modulation in thrombotic disorders. Thromb Haemost. 2014 Jul 3;112(1):32-42. DOI: 10.1160/TH14-01-003210.1160/TH14-01-003224671700Search in Google Scholar

24. Broos K, Feys HB, De Meyer SF, Vanhoorelbeke K, Deckmyn H. Platelets at work in primary hemostasis. Blood Rev. 2011 Jul;25(4):155-67. DOI: 10.1016/j.blre.2011.03.00210.1016/j.blre.2011.03.00221496978Search in Google Scholar

25. Available from: http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=1800790. [cited 2015.07.15].Search in Google Scholar

26. Available from: http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=5918. [cited 2015.08.05].Search in Google Scholar

27. Pongracz E, Scweitzer K, Furesz J, Fent J, Tordai A, Nagy Z. The effects of platelet receptor GPIIb/IIIa polymorphism (Leu Pro33) on the receptor expression and platelet aggregation in patients with ishaemic stroke. Turk J Hematol. 2007 Sep;24(4):155-163.Search in Google Scholar

28. Guo J, Liu A, Su D. Genetics of stroke. Acta Pharm Sinic 2010 Aug; 31:1055–1064. DOI: 10.1038/aps.2010.14110.1038/aps.2010.141400231620729874Search in Google Scholar

29. Siengerink B, Rosendaal F.R, Algra A. Genetic variation in fibrinogen; its relantionship to fibrinogen levels and the risk of myocardial infarction and ischemic stroke. J Thromb Haemost. 2009 Mar;7(3):385-90. DOI: 10.1111/j.1538-7836.2008.03266.x10.1111/j.1538-7836.2008.03266.x19143925Search in Google Scholar

30. Lee SH, Kim MK, Park MS, Choi SM, Kim JT, Kim BC, et al. Beta-fibrinogen -455 G/A polymorphism in Korean ischemic stroke patients. J Clin Neurol. 2008 Mar;4(1):17-22. DOI: 10.3988/jcn.2008.4.1.1710.3988/jcn.2008.4.1.17268688319513319Search in Google Scholar

31. Golenia A, Chrzanowska-Wasko J, Jagiella J, Wunk M, Ferens A, Klimkowicz-Mrowiec A, et al. The β-fibrinogen -455G/A gene polymorphism and the risk of ischaemic stroke in a Polish population. Neurol Neurochir Pol. 2013 Mar-Apr;47(2):152-6. DOI: 10.5114/ninp.2013.3446210.5114/ninp.2013.3446223650004Search in Google Scholar

32. Kessler C, Spitzer C, Stauske D, Mende S, Stadlmuller J, Walther R, et al. The apolipoprotein E and beta-fibrinogen G/A-455 gene polymorphisms are associated with ischemic stroke involving large vessel disease. Arterioscler Thromb Vasc Biol. 1997 Nov;17(11):2880-4. DOI: 10.1161/01.ATV.17.11.288010.1161/01.ATV.17.11.2880Search in Google Scholar

33. Liu Y, Pan J, Wang S, Li X, Huang Y. Beta-fibrinogen gene -455A/G polymorphism and plasma fibrinogen level in Chinese stroke patients. Chin Med J (Engl). 2002 Feb;115(2):214-6.Search in Google Scholar

34. Ridker PM, Hennekens CH, Schmitz C, Stampfer MJ, Lindpaintner K. Lancet. PIA1/A2 polymorphism of platelet glycoprotein IIIa and risks of myocardial infarction, stroke, and venous thrombosis. Lancet. 1997 Feb 8;349(9049):385-8. DOI: 10.1016/S0140-6736(97)80010-410.1016/S0140-6736(97)80010-4Search in Google Scholar

35. Li Q, Chen BL, Ozdemir V, Ji W, Mao YM, Wang LC, et al. Frequency of genetic polymorphisms of COX1, GPIIIa and P2Y1 in a Chinese population and association with attenuated response to aspirin. Pharmacogenomics. 2007 Jun;8(6):577-86. DOI: 10.2217/14622416.8.6.57710.2217/14622416.8.6.57717559347Search in Google Scholar

36. Floyd CN, Mustafa A, Ferro A. The PlA1/A2 polymorphism of glycoprotein IIIa as a risk factor for myocardial infarction: a meta-analysis. PLoS One. 2014 Jul 2;9(7):e101518. DOI: 10.1371/journal.pone.010151810.1371/journal.pone.0101518407971724988220Search in Google Scholar

37. Carter AM, Catto AJ, Bamford JM, Grant PJ. Platelet GP IIIa PlA and GP Ib variable number tandem repeat polymorphisms and markers of platelet activation in acute stroke. Arterioscler Thromb Vasc Biol. 1998 Jul;18(7):1124-31. DOI: 10.1161/01.ATV.18.7.112410.1161/01.ATV.18.7.11249672073Search in Google Scholar

38. Lanni F1, Santulli G, Izzo R, Rubattu S, Zanda B, Volpe M, et al. The Pl(A1/A2) polymorphism of glycoprotein IIIa and cerebrovascular events in hypertension: increased risk of ischemic stroke in high-risk patients. J Hypertens. 2007 Mar;25(3):551-6. DOI: 10.1097/HJH.0b013e328013cd6710.1097/HJH.0b013e328013cd6717278970Search in Google Scholar

39. Oksala NK1, Heikkinen M, Mikkelsson J, Pohjasvaara T, Kaste M, Erkinjuntti T, et al. Smoking and the platelet fibrinogen receptor glycoprotein IIb/IIIA PlA1/A2 polymorphism interact in the risk of lacunar stroke and midterm survival. Stroke. 2007 Jan;38(1):50-5. DOI: 10.1161/01.STR.0000251719.59141.3610.1161/01.STR.0000251719.59141.3617138951Search in Google Scholar

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