<abstract xml:lang="en"><title style='display:none'>Physicochemical Properties of Honey Produced in the Homesha District of Western Ethiopia</title>This study was conducted to characterize honey produced in the Homesha district in Western Ethiopia. The effects of location and hive type on the quality of honey produced in the area were assessed. A total of 20 honey samples were collected from four locations in the district. The overall mean (±SD) contents of reducing sugars, sucrose, pH, moisture, ash, hydroxymethylfurfural, acidity and water-insoluble solids of the honey samples analyzed were 65 ± 3.02%, 6.1 ± 3.4%, 4.02 ± 0.26, 16.4 ± 1.07%, 0.17 ± 0.07%, 1.8 ± 0.24 mg/kg, 23.9 ± 7.4 meq/kg and 0.09 ± 0.14%, respectively. The sucrose content of honey samples purchased from the market was significantly higher (p&lt;0.05) than the sucrose contents of honey samples collected from other locations. Hive type significantly (p&lt;0.05) influenced the contents of moisture, HMF and water-insoluble solids of honey samples. It can be concluded that honey produced in the Homesha district is of good quality and can meet the national and international market demands.</abstract>

Department of Animal and Fish Resource Development, Bureau of Agriculture and Rural Development, Benishangul Gumuz Regional State, P.O. Box 30, Assosa, Ethiopia

Department of Animal Sciences, Haramaya University, P.O. Box 287, Haramaya campus, Ethiopia

Honeybee Research Program, Holetta Agricultural Research Center, P.O. Box 22, Holetta, Ethiopia

Physicochemical Properties of Honey Produced in the Homesha District of Western Ethiopia

<abstract xml:lang="en"><title style='display:none'>Histological structure of the Midgut of honey bees (<italic>Apis Mellifera</italic> L.) Fed Pollen Substitutes Fortified with Probiotics</title>The aim of this investigations was to assess morphological changes in the midgut epithelium of bees nourished with pollen substitute or pollen substitute enriched with a probiotic preparation. One-day old worker bees were kept in cages placed in a temperature controlled environment. During the two-week feeding period workers were fed beebread (control), pure pollen substitute or pollen substitute fortified with three different doses of probiotic preparations: Biogen or Trilac (experimental groups). The assessment of histological changes of the bee midgut was carried out in bees feed for 8 and 14 days. Slight changes in the epithelium as well as strong merocrine-type secretion were recorded in bees nourished pollen substitute supplemented with probiotic preparations. Differences were observed, primarily, in quantities of the developed peritrophic membranes. Their quantities were particularly high after 14 days of feeding with the pollen substitute fortified with probiotic preparations. The development of numerous peritrophic membranes could have contributed to better utilization of nutrients contained in feed and better condition of bees.</abstract>

Histological structure of the Midgut of honey bees (<italic>Apis Mellifera</italic> L.) Fed Pollen Substitutes Fortified with Probiotics

<abstract><title style='display:none'>Abstract</title> Experiments were conducted in three apiaries to assess the comparative efficacy of: Thymovar (Andermatt BioVet AG); Apiguard (Vita Europe Ltd., UK); an oxalic acid solution (OA) which consisted of 2.9% oxalic acid and 31.9% sugar in water; and amitraz fumigation, for controlling the honey bee mite <italic>Varroa destructor</italic>. Mite mortality increased significantly (p&lt;0.001) in the Thymovar, Apiguard, OA or amitraz treated colonies. The relative mite mortality after: four OA applications, two Thymovar or two Apiguard applications during August and September in the Senično apiary was 41.80% (±14.31), 14.35% (±10.71), and 18.93% (±13.56), respectively. In the control, i.e. untreated colonies, the mite natural mortality was reduced by 3.10% (±3.50). In the Bohinj apiary, two Apiguard applications and a single amitraz treatment resulted in reducing the mite populations by 19.71% (±12.61) and 23.89% (±14.25), respectively. At the Mediterranean located apiary of Vipava, the Thymovar and Apiguard treatments trigged 59.02% (±17.28) and 46.50% (±13.33) of the total mite reduction. In the Vipava apiary, colonies treated with any miticide during the brood period presented no difference (P&gt;0.05) in efficacy. The results indicate that OA, Thymovar, Apiguard or amitraz fumigations are of limited use during the brood periods.</abstract>

Agricultural Institute of Slovenia, Hacquetova 17, SI-1000 Ljubljana

University of Maribor, Faculty of Agriculture and Life Sciences, Slovenia

Veterinary Faculty of the University of Ljubljana, National Veterinary Institute, Ljubljana, Slovenia

Use of Thymol Formulations, Amitraz, and Oxalic Acid for the Control of the Varroa Mite in Honey Bee (Apis mellifera carnica) Colonies

<abstract xml:lang="en"><title style='display:none'>Review of the Expression of Antimicrobial Peptide Defensin in Honey Bees <italic>Apis Mellifera</italic> L.</title>Honey bees defensin have a high level of polymorphism and exist as two peptides - defensin 1 and 2. Defensin 1 is synthesized in the salivary glands and is responsible for social immunity. Defensin 2 is synthesized by cells of the fat body and hemolymph is responsible for individual immunity. Defensins are inducible and controlled by the interaction of Toll and Imd signaling pathways and have a broad spectrum of antimicrobial action. The use of chitosan as an immunomodulator has been shown to lead to an increase in the expression levels of defensin and abaecin in the honey bee organism. Stimulation of the transcriptional activity of the defensin genes will allow for the control of a honey bee colony's immunity level, and reduce the using of antibiotics and other chemicals.</abstract>

Review of the Expression of Antimicrobial Peptide Defensin in Honey Bees <italic>Apis Mellifera</italic> L.

<abstract><title style='display:none'>Summary</title>Amitraz is a varroacide used the longest (over thirty years) and most commonly by Polish beekeepers. This involves high risk of V. destructor developing amitraz resistance. Therefore it is necessary to monitor the effectiveness of treatments to ensure appropriate protection of the honeybee colonies. The objective of this study was to evaluate the efficacy of amitraz used as the contact varroacide (Biowar 500 formulation) to control V. destructor in honeybee colonies. Field studies were conducted in 2011 and 2012. In both years, the amitraz treatments started in the last weeks of August. Two strips of Biowar 500 (500 mg of amitraz per strip) were inserted into each colony and removed after 8 weeks. The control colonies were left untreated for 8 weeks. After removing the strips the control therapy was conducted in all colonies. The average efficacy of amitraz (E%) calculated for the two years combined, after 6 and 8 weeks of treatment, amounted to 90.6% and 94.6%, respectively. As a result of the natural mortality in the control colonies, the population of parasites lowered only by 16.4% and 23.9% during 6 and 8 weeks. The efficacy of the strips was lower in colonies with larger amount of brood.</abstract>

Research Institute of Horticulture, Apiculture Division, Puławy, Poland

National Veterinary Research Institute, Department of Honey Bee Diseases, Puławy, Poland

The Amitraz Strips Efficacy in Control of Varroa Destructor After Many Years Application of Amitraz in Apiaries

<abstract><title style='display:none'>Abstract</title>The paper presents the results of rheological measurements conducted on three types of Polish honey: rape, multi-floral, and buckwheat honeys. The investigations involved identification of the properties of the honeys in both liquefied (by heating) as well as crystallised states. Both steady shear as well as dynamic rheological tests were performed. As a result, it was possible to show that the liquefied honeys behave like Newtonian fluids. Good agreement of the results between the rotary shear and oscillation rotary tests was observed, thus fulfilling the Cox-Mertz rule. The structure of the honeys was subjected to qualitative scrutiny by analysing photographs of the crystals taken in the conditions of shearing interferometry. The quantitative analysis was made by presenting a numerical distribution of crystal colonies with reference to the maximum dimensions of individual crystals. The geometric measurements of the crystals were carried out using analiSIS software. In the crystallised form, the media showed a thixotropic effect, and their apparent viscosity was many times higher than the dynamic viscosity in the liquid state. After plasticising by deformation with an increasing shear rate of up to 450s−1, the equilibrium melting curves of the crystallised honeys were described by the Ostwald-de Waele model. One particular reason for the research was to show that the results obtained for the honeys crystallised by the steady shear method, were qualitatively different from the results obtained in the dynamic measurements. The Cox-Mertz rule cannot be applied for the crystallised honeys.</abstract>

Białystok University of Technology, Faculty of Forestry in Hajnówka

Rheological Properties of Some Honeys in Liquefied and Crystallised States

<abstract>
<title style='display:none'>Abstract</title>
Honey bee populations in the United States have been under stress for the past several decades. Several internal parasites may contribute to this, including the trypanosome <italic>Lotmaria passim</italic>. It is unknown how widespread the honey bee parasite, <italic>L. passim</italic>, is in Africanized honey bee (AHB) populations in the United States. A total of 321 feral honey bee colony samples which had been previously recorded to be of Africanized origin using molecular diagnostics from California (n=3), New Mexico (n=46), Oklahoma (n=57), Texas (n=106), and Utah (n=109) were used in this study. Of these samples, a total of 15 (4.7%) from three States were positive for <italic>L. passim</italic>. Utah AHB samples had the highest infection rate (11.0%), followed by Texas (1.9%) and Oklahoma (1.8%). Compared with previous studies on the occurrence of <italic>L. passim</italic> from European honey bees from the same sampled States, infection rates of Africanized honey bees for <italic>L. passim</italic> were significantly higher in the State of Utah, but not for the other sampled States. This study provides evidence that feral honey bee populations do not necessarily have lower levels of honey bee parasites than managed honey bee colonies.
</abstract>

Occurrence of <italic>Lotmaria Passim</italic> in Africanized and European Honey Bee, <italic>Apis Mellifera</italic>, Lineages from the United States

<abstract>
<title style='display:none'>Abstract</title>
The three honey bee phenotypes - queens, workers, and drones differ in the size and shape of body parts. We used a large dataset to describe how the three phenotypes differed with regard to fore wings and applied the methodology of geometric morphometrics to measure the wings using the coordinates of nineteen landmarks. On average, queens had wings larger than workers, but the two castes had a broad overlap. Drones, on the other hand, had distinctly larger wings. Wing shape differed markedly among queens, workers and drones and can be used to reliably differentiate them from one other. Surprisingly, the fore wing shape of workers was more similar to that of drones than to that of queens. Small queens were not more similar to workers than large queens, and large workers were not more similar to queens than small workers. Because wing size, unlike body mass, does not change over the life of the queen, it can be used to evaluate the quality of a queen throughout its life and not only during a short period after emergence. We provide a large number of wing images of queens and drones, which can be used as a reference in future studies.
</abstract>

Size and Shape Differences in Fore Wings of Honey Bee (Apis Mellifera) Queens, Workers and Drones

<abstract>
<title style='display:none'>Abstract</title>
The virulence patterns observed in viruses transmitted by <italic>Varroa destructor</italic> implied a potential association of these honey bee pests in Poland with the Korean haplotype. Nevertheless, it is noteworthy that this hypothesis lacked direct verification. Therefore, the aim of the study was to determine the haplotype affiliation of <italic>V. destructor</italic> mites infesting bees in Poland and Ukraine. Adult females were collected from drone brood from honey bee colonies maintained in the apiary at the University of Life Sciences in Lublin, Poland, and the apiary in Synevyr National Park in Ukraine. The haplotype affiliation was determined through the sequencing of the cytochrome oxidase subunit I gene (CO I; 929 bp) fragment. Based on the phylogenetic comparison with 84% bootstrap support, the <italic>V. destructor</italic> females from both locations were confirmed to represent three haplotypes: Korean 1 (AmK1-1), Korean 2 (AmK1-2), and Chinese 4 (AmK1-4). They are the most virulent types of this parasite worldwide and in Poland. Concurrently, the present study confirms that the Synevyr National Park in Ukraine is isolated from alien bee subspecies, but is not free from alien parasites.
</abstract>

Haplotype Affiliation of <italic>Varroa Destructor</italic> in Selected Locations From Poland and Ukraine

<abstract>
<title style='display:none'>Abstract</title>
Honey bee workers (<italic>Apis mellifera</italic> L.) collect nectar, honeydew and pollen from plants in orderto provide the colony with, among others, carbohydrates and protein. Whenever these sources are unavailable in the environment, bees turn to alternatives. An example of this are fungi spores collected accidentally or on purpose. This last phenomenon is the aim of this study, in which we have shown that worker bees can collect willow rust (<italic>Melampsora</italic> spp.) spores. We observed as the bees obtained spores and placed them in pollen baskets. The presence of spores was demonstrated with the use of a scanning electron microscope (SEM). This observation indicates that the honey bee may use alternative sources as a potential supplement. There are few studies in this field and it requires a deeper analysis.
</abstract>

Case of Willow Rust Spores (<italic>Melampsora</italic> Spp.) Collected by Honey Bees

<abstract>
<title style='display:none'>Abstract</title>
Eight samples of propolis commercially available on south-eastern Poland’s beekeeping market were compared in terms of quality, chemical composition and biological activity, including antioxidant and antimicrobial properties. The organoleptic characteristics and physicochemical requirements indicated that only 50% of tested propolis samples was classified to class II regarding obligatory limits, while the others were out-class. A big variation in mineral composition was determined by the ICP-OES method but all samples were free of heavy metals (Pb, Cd and Ni). Although mesophilic bacteria, yeasts and moulds occurred in tested propolis (in general lower than 102 CFU/g), <italic>E. coli</italic> and spore-forming sulphite-reducing bacteria as well as <italic>Salmonella</italic> and <italic>Shigella</italic> bacteria were not detected. High antioxidant activity and total phenolic content for all samples was confirmed, which was correlated with antibacterial activity tested against <italic>E. coli</italic> ATCC 11775 strain. Additionally, the propolis sample with the strongest antibacterial activity (MIC 0.33 mg/mL) inhibited biofilm formation of <italic>S. aureus</italic> and <italic>S. epidermidis</italic> ATCC 35984 (MBIC 0.66 and 5.25 mg/mL, respectively). However, great variation in terms of antioxidant activity and HPLC polyphenolic profile were observed, and sakuranetin and pinobanksin were identified as the most effective antioxidant components. Moreover, the usefulness of ATR-FTIR spectra analysis was confirmed as a quick method for initial testing of propolis quality.
</abstract>

Quality of Propolis Commercially Available on Podkarpacki Beekeeping Market

<abstract>
<title style='display:none'>Abstract</title>
This study aimed to assess the effect of botanical and geographical differences on the protein, minerals, total phenolic content and the antioxidant activity of bee pollen collected from five districts in West Shewa Zone, Ethiopia. Pollens of <italic>Guizotia</italic> sp., <italic>Brassica</italic> sp., <italic>Trifolium</italic> sp., <italic>Eucalyptus</italic> sp., <italic>Plantago lanceolata</italic> and <italic>Vicia faba</italic> were color-sorted from multifloral pollens and subjected to chemical analysis. The results revealed the mean content of moisture 10.6% of fresh weight (FW), protein 19.4% of dry weight (DW) and ash 2.4% (DW); the most dominant minerals, K (9765.6), Ca (963.4), Mg (960.5), Fe (142.8) and P (126.5) in mg/kg DW. The average of the total phenolic content (TPC) and the antioxidant activity (DPPH radical) were 40.3 GAE mg/g FW and 13.7 AAE mg/g FW (gallic and ascorbic acid equivalent, respectively). <italic>V. faba</italic> pollen had the highest protein, ash, P and Cu content. Four pollen taxa had the highest Ca values, while multifloral pollens contained the highest Fe content. The samples from Ejere had the highest ash, Ca, Na and Cu content. The results showed a high content of the tested chemicals and a strong antioxidant capacity of bee pollen, varying with botanical and geographical origins. These findings would also serve as inputs for further assessment of the nutritional values of pollen for honey bee colonies and potential human consumption. However, comprehensive chemical investigations of larger number of samples representing diversified flora and locations are crucial to developing a broad regional information base on the nutritional profile of bee pollen.
</abstract>

Proximate Composition and Antioxidant Activity of Honey Bee Collected Pollen in the Main Flowering Season, in West Shewa Zone, Central Ethiopia

<abstract><title style='display:none'>Abstract</title> The risk exposure of bee colonies to the toxicity of systemic neonicotinoid insecticides was assessed. Various methods of chemical prevention of commercial winter and spring oilseed rape crops in field-realistic conditions were taken into account in the assessment. Pesticides were applied in accordance with the actual agricultural practice. Commercial crop protection products with thiamethoxam, clothianidin or imidacloprid were used as seed treatment. Formulations containing acetamiprid or thiacloprid were used for spraying. Fifteen healthy bee colonies were placed in close proximity to each of the oilseed rape fields throughout the blooming period. During florescence, the samples of nectar (directly from flowers and nectar flow from combs) and pollen loads were collected repeatedly. Samples of honey, bee bread and adult bees were taken one week after the end of plants flowering. To ensure high specificity and sensitivity of analysed pestcicides modified QuEChERS extraction method and liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) was used. The five of neonicotinoid insecticides (imidacloprid, clothianidin, thiametoxam, acetamiprid and thiacloprid) were analyzed in multi-residue method with 0.1 - 10 ng/g limits of detection. Palynological analysis was done to determine the botanical origin of the nectar, honey and pollen. Development of bee colonies (brood area, worker biomass, colony health) was assessed every 3 weeks until the end of the beekeeping season. The amount of pollen collected by bees per hive, bee bread area and rape honey yield was also measured. The long-term effects of insecticides on bees were estimated using the same methods in April of the following year. All the neonicotinoid insecticides applied to control oilseed rape pests were present in the samples of nectar and pollen. Their residue levels were lower than the acute oral and contact LD50 values. Among five examined neonicotinoids, the most frequently detected were: thiamethoxam, thiacloprid and acetamiprid. These substances were present in 65, 64, and 51% of the nectar samples and in 37, 62, and 45% of the pollen samples, respectively. The highest level of residues were noted after the thiamethoxam seed treatment; on average, 4.2 and 3.8 ng/g in the nectar and pollen samples. In the nectar and pollen samples from winter rape fields, lower levels of neonicotinoid residues were found in comparison to spring rape samples. The contaminations of neonicotinoids applied as seed dressing in nectar samples were significantly higher in comparison to the pollen samples. No negative effects of neonicotinoids on the bee mortality, brood development, strength, and honey yield of healthy bee colonies were found throughout the study period. However, the risk exposure of bee colonies on adverse impact of pesticide residues is high in areas of intensively cultivated oilseed rape.</abstract>

Research Institute of Horticulture, Food Safety Laboratory, Skierniewice, Poland

Residues of Neonicotinoid Insecticides in Bee Collected Plant Materials from Oilseed Rape Crops and their Effect on Bee Colonies

<abstract><title style='display:none'>Abstract</title> Although beeswax adulteration represents one of the main beeswax quality issues, there are still no internationally standardised analytical methods for routine quality control. The objective of this study was to establish an analytical procedure suitable for routine detection of beeswax adulteration using FTIR-ATR spectroscopy. For the purpose of this study, reference IR spectra of virgin beeswax, paraffin, and their mixtures containing different proportions of paraffin (5 - 95%), were obtained. Mixtures were used for the establishment of calibration curves. To determine the prediction strength of IR spectral data for the share of paraffin in mixtures, the Partial Least Squares Regression method was used. The same procedure was conducted on beeswax-beef tallow mixtures. The model was validated using comb foundation samples of an unknown chemical background which had been collected from the international market (n = 56). Selected physico-chemical parameters were determined for comparison purposes. Results revealed a strong predictive power (R2 = 0.999) of IR spectra for the paraffin and beef tallow share in beeswax. The results also revealed that the majority of the analysed samples (89%) were adulterated with paraffin; only 6 out of 56 (11%) samples were identified as virgin beeswax, 28% of the samples exhibited a higher level of paraffin adulteration (&gt;46% of paraffin), while the majority of the analysed samples (50%) were found to be adulterated with 5 - 20% of paraffin. These results indicate an urgent need for routine beeswax authenticity control. In this study, we demonstrated that the analytical approach defining the standard curves for particular adulteration levels in beeswax, based on chemometric modelling of specific IR spectral region indicative for adulteration, enables reliable determination of the adulterant proportions in beeswax.</abstract>

University of Zagreb Faculty of Agriculture, Department of Fisheries, Apiculture and Special Zoology, 10000 Zagreb, Croatia

Rudjer Boskovic Institute, Division of Organic Chemistry and Biochemistry (Laboratory of Molecular Spectroscopy), 10000 Zagreb, Croatia

University of Zagreb Faculty of Agriculture, Department of Chemistry, 10000 Zagreb, Croatia

University of Zagreb, Faculty of Veterinary Medicine, Department of Biology and Pathology of Fish and Bees, 10000 Zagreb, Croatia

An Approach for Routine Analytical Detection of Beeswax Adulteration Using FTIR-ATR Spectroscopy

<abstract><title style='display:none'>Abstract</title> Beebread is processed pollen stored in the cells of the honeycomb, with the addition of various enzymes and honey, which undergoes lactic acid fermentation. Ethanolic extracts (EBBs) were obtained from three different samples of beebread from Poland. Assays were carried out for the determination of chemical composition (GC/MS), for the total phenolic content, and for the antioxidant and cytotoxic activities. The effects of beebread extracts (10, 20, 30, 50, 100 μg/mL) on the viability of the glioblastoma cell line (U87MG) were studied after 24 h, 48 h, and 72 h. Our results indicated a time-dependent inhibitory effect on the viability of U87MG cells treated EBB. The main inhibitory effect of EBB was observed after 72 h; EBB treatment decreased cell viability to 49 - 66%.</abstract>

Department of Bromatology, Medical University of Białystok, Poland

Institute of Chemistry, University of Białystok, Poland

Chemical composition and antioxidant activity of beebread, and its influence on the glioblastoma cell line (U87MG)

<abstract><title style='display:none'>Abstract</title>This study was conducted at the apiary of the Agricultural and Veterinary Training and Research Station, King Faisal University, Al-Ahsa, Saudi Arabia. The purpose was to study the relationship between the botanical origin and chemical composition of bee-collected pollen. The amount of mineral elements present in bee-collected pollen was also studied. The composition of pollen loads showed the maximum contents of dry matter, ash, glucose, fructose, magnesium (Mg), phosphorus (P), and manganese (Mn) for the date palm; the maximum contents of protein, calcium (Ca), and zinc (Zn) for alfalfa; the maximum contents of fiber, and copper (Cu) for the sunflower; the maximum contents of the lipids and iron (Fe) for summer squash; and the maximum contents of sodium (Na), and potassium (K) for rape. Calcium was found to be correlated in a significantly (p&lt;0.01) positive way with K, Na, Mg, P, Mn, and Zn. Copper, though, was correlated in a significantly (p&lt;0.01) negative way with Ca, Mg, P, Mn, and Zn. The high levels of protein, ash, glucose, and fructose, and low lipid content found in bee-pollen from the date palm and from alfalfa, mean that pollen from the date palm and from alfalfa make an excellent food supplement. It was concluded, that the chemical composition of beecollected pollen can be correlated with the plant species from which pollen was collected.</abstract>

Department of Arid Land Agriculture, College of Agriculture and Food Sciences, King Faisal University, Al-Hasa, Saudi Arabia

Department of Economic Entomology, Faculty of Agriculture, Kafrelsheikh University,Kafrelsheikh, Egypt

Chemical Composition and Amounts of Mineral Elements in Honeybee-Collected Pollen in Relation to Botanical Origin

<abstract><title style='display:none'>Abstract</title>We examined the influence of curcumin-supplemented feeding on worker lifespan, Nosema resistance, key enzyme activities, metabolic compound concentrations and percentage of the global DNA methylation. Two worker groups (Apis mellifera) were set up: 1) control group; workers were fed ad libitum with sucrose syrup; 2) workers were fed with the syrup with the addition of curcumin. Dead workers were removed every two days and the Nosema spp. infection levels were assessed. Hemolymph was taken from living workers for biochemical analyses. The global DNA methylation level was analysed using DNA from worker heads and thoraces. The bees that consumed curcumin lived longer and were less infested with Nosema spp. The curcumin-treated workers had higher concentrations of proteins, non-enzymatic biomarkers (triglycerides, glucose, cholesterol, Mg2+ and Ca2+), uric acid and creatinine, as well as elevated activities of antioxidant enzymes (SOD , GPx, CAT , GST ), neutral proteases, protease inhibitors, enzymatic biomarkers (AST , ALT , ALP ). The concentrations of albumin and urea, and the activities of acidic and alkaline proteases were higher in the control group. Curcumin decreased global DNA methylation levels especially in older bees in which the natural, age-related level increase was observed. Most of the parameters increased over the apian youth and adulthood, and decreased in older bees. The decrease was markedly delayed in the bees fed with curcumin. Curcumin appeared to be an unexpectedly effective natural bio-stimulator, improving apian health and vitality. This multifactorial effect is caused by the activation of many biochemical processes involved in the formation of apian resistance.</abstract>

Curcumin Stimulates Biochemical Mechanisms of Apis Mellifera Resistance and Extends the Apian Life-Span

<abstract><title style='display:none'>Abstract</title>The objective of the present work was to investigate the influence of locations on bioactive propertiest, phenolic compounds and mineral contents of bee pollens. The oil content of pollen grains changed between 3.50% (Alanya) and 6.85% (Russia-Perm Region). The highest total phenolic content (720 mg/100g) and antioxidant activity values (81.4%) were observed in pollens obtained from the Russia-Perm Region and Alanya districts, respectively. Additionally, the highest carotenoid was found in a pollen sample collected from Karaman (Sarıveliler) (98.6 mg/g). The major phenolic compounds were (+)-catechin (66.75-337.39 mg/100g) and quercetin (61.2-1221.7 mg/100g) in all pollen samples. The pollen samples were observed to be a significant source of potassium (3846-6287 mg/kg), phosphorus (2947-5010 mg/kg), calcium (1022-2424 mg/kg) and sulfur (1744-2397 mg/kg). All of the analysis results were significantly affected by supplying locations. The antioxidant activity values of pollens were found partly similar and varied depending on locations. The content of saturated fatty acid (palmitic) was high (20-30%) in the tested pollen samples but did not exceed the content of linoleic acid.</abstract>

Determination of Antioxidant Activity, Phenolic Compound, Mineral Contents and Fatty Acid Compositions of Bee Pollen Grains Collected from Different Locations

Guidelines for Reviewers

Guidelines for Authors