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Inhibitory effect of dimethyl sulfoxide on the development of gastrointestinal nematode larvae in the larval development test

Marcin Mickiewicz

Mickiewicz, Marcin

,

,

Michał Czopowicz

Czopowicz, Michał

,

Agata Moroz-Fik

Moroz-Fik, Agata

,

Adrian-Valentin Potărniche

Potărniche, Adrian-Valentin

,

Kinga Biernacka

Biernacka, Kinga

,

Olga Szaluś-Jordanow

Szaluś-Jordanow, Olga

,

Górski, Paweł

,

Alistair Antonopoulos

Antonopoulos, Alistair

,

Iwona Markowska-Daniel

Markowska-Daniel, Iwona

,

Marián Várady

Várady, Marián

und

Kaba, Jarosław

25. März 2025

Inhibitory effect of dimethyl sulfoxide on the development of gastrointestinal nematode larvae in the larval development test's Cover Image

Journal of Veterinary Research

Band 69 (2025): Heft 1 (März 2025)

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Online veröffentlicht: 25. März 2025

Seitenbereich: 83 - 90

Eingereicht: 12. Sept. 2024

Akzeptiert: 11. März 2025

DOI: https://doi.org/10.2478/jvetres-2025-0016

Schlüsselwörter
anthelmintic resistance, DMSO, five-parameter logistic curve, inhibitory concentration, Trichostrongylidae

© 2025 Marcin Mickiewicz et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

The observed percentage of unhatched eggs, larvae of the first and second stage (L1-L2) and larvae of the third stage (L3; percentage development) of Haemonchus contortus, Trichostrongylus spp. and Oesophagostomum spp. at increasing dimethyl sulfoxide (DMSO) concentrations. Dots – arithmetic means; whiskers – 95% confidence intervals; asterisks (*) – statistically significantly lower percentages than in the control wells according to Dunnett’s test at α = 0.05

The five-parameter logistic curve (blue line) fitted to the observed percentage development of larvae of the third stage (L3) of Haemonchus contortus, Trichostrongylus spp. and Oesophagostomum spp. at increasing dimethyl sulfoxide (DMSO) concentrations. Red dots – exact values of tested concentrations indicated. Orange broken lines and arrows – DMSO concentrations inhibiting the development of 10%, 50% and 90% of L3

The proportion of developed larvae of the third stage (L3) of Haemonchus contortus, Trichostrongylus spp. and Oesophagostomum spp. (out of 100 counted L3) at increasing dimethyl sulfoxide (DMSO) concentrations. P-values of McNemar’s test

The observed percentage of eggs, larvae of the first and second stage (L1 and L2) and larvae of the third stage (L3; percentage development – PD) at increasing dimethyl sulfoxide (DMSO) concentrations

DMSO concentration		Percentage of developmental stages observed after the 7-day incubation (%) ^a
	n	L3 (PD)		Unhatched eggs		L1 and L2
	n		P-value ^b		P-value ^b		P-value ^b
0.0% (control)	30	95.5 ± 3.1 (87.5–100)	-^c	0.05 ± 0.28 (0–1.54)	-^c	4.43 ± 3.08 (0–12.5)	-^c
0.6%	30	97.0 ± 2.6 (89.2–100)	0.856	0.99 ± 1.60 (0–8.11)	0.022*	1.99 ± 2.26 (0–7.9)	0.450
1.3%	30	92.8 ± 4.4 (81.0–100)	0.335	0.85 ± 1.27 (0–4.11)	0.068	6.35 ± 4.40 (0–19.0)	0.683
2.6%	30	76.3 ± 8.8 (50.0–91.8)	<0.001*	0.49 ± 0.90 (0–3.13)	0.566	23.2 ± 8.8 (8.2–50.0)	<0.001*
5.2%	30	27.6 ± 10.4 (10.8–50.0)	<0.001*	0.74 ± 1.43 (0–5.41)	0.157	71.7 ± 10.5 (50.0–89.2)	<0.001*
10.4%	30	5.7 ± 6.6 (0–33.7)	<0.001*	1.21 ± 1.53 (0–5.97)	0.003*	93.1 ± 6.6 (66.3–100)	<0.001*
20.8%	30	0.04 ± 0.22 (0–1.2)	<0.001*	0.84 ± 1.27 (0–4.62)	0.073	99.1 ± 1.3 (95.4–100)	<0.001*

Sprache:: Englisch

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